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Citation for Study 12076

Citation title: "Identification and Characterization of Chitin Synthase Genes in the Postharvest Citrus Fruit Pathogen Penicillium digitatum.".

Study name: "Identification and Characterization of Chitin Synthase Genes in the Postharvest Citrus Fruit Pathogen Penicillium digitatum.".

This study is part of submission 12076 (Status: Published).

Citation

Gand?a M., Harries E., & Marcos J.F. 2012. Identification and Characterization of Chitin Synthase Genes in the Postharvest Citrus Fruit Pathogen Penicillium digitatum. Fungal Biology , .

Authors

• Gand?a M.
• Harries E.
• Marcos J.F.

Abstract

In this study, we carried out the isolation and characterization of chitin synthase genes (CHS) of the main citrus fruit postharvest pathogen Penicillium digitatum. Using distinct sets of degenerate primers designed from conserved regions of CHS genes of yeast and filamentous fungi, PCR methods and a DNA genomic library, five putative CHS genes (PdigCHSI, PdigCHSII, PdigCHSIII, PdigCHSV and PdigCHSVII) were identified, isolated, sequenced, and characterized. Phylogenetic analyses, sequence identity, and domain conservation support the annotation as CHS. A very high sequence identity and strong synteny were found with corresponding regions from the genome of Penicillium chrysogenum. Gene expression of P. digitatum CHS genes during mycelium axenic growth, under oxidative and osmotic stress conditions, and during infection of citrus fruits was confirmed and quantified using quantitative RT-PCR (qRT-PCR). PdigCHSIII had the highest expression among the five genes by one order of magnitude, while PdigCHSII had the lowest. However, PdigCHSII was strongly induced coincident with conidial production, suggesting a role in conidiogenesis. The expression of PdigCHSI, PdigCHSIII, PdigCHSV, and PdigCHSVII was upregulated during infection of citrus fruit. PdigCHSV and PdigCHSVII coexpressed in most of the experiments carried out, and they are separated by a 1.77 kb intergenic region and arranged in opposite directions

Keywords

Penicillium digitatum, Chitin synthase, Cell wall, Gene expression, qRT-PCR, Transcriptional regulation

External links

• DOI: 10.1016/j.funbio.2012.03.005
• Other URL: http://www.sciencedirect.com/science/article/pii/S1878614612000645

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S12076
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref20180,
  	 author = {M?nica  Gand?a and Eleonora  Harries and Jose F. Marcos},
  	 title = {Identification and Characterization of Chitin Synthase Genes in the Postharvest Citrus Fruit Pathogen Penicillium digitatum.},
  	 year = {2012},
  	 keywords = {Penicillium digitatum, Chitin synthase, Cell wall, Gene expression, qRT-PCR, Transcriptional regulation},
  	 doi = {10.1016/j.funbio.2012.03.005},
  	 url = {http://www.sciencedirect.com/science/article/pii/S1878614612000645},
  	 pmid = {},
  	 journal = {Fungal Biology },
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {In this study, we carried out the isolation and characterization of chitin synthase genes (CHS) of the main citrus fruit postharvest pathogen Penicillium digitatum. Using distinct sets of degenerate primers designed from conserved regions of CHS genes of yeast and filamentous fungi, PCR methods and a DNA genomic library, five putative CHS genes (PdigCHSI, PdigCHSII, PdigCHSIII, PdigCHSV and PdigCHSVII) were identified, isolated, sequenced, and characterized. Phylogenetic analyses, sequence identity, and domain conservation support the annotation as CHS. A very high sequence identity and strong synteny were found with corresponding regions from the genome of Penicillium chrysogenum. Gene expression of P. digitatum CHS genes during mycelium axenic growth, under oxidative and osmotic stress conditions, and during infection of citrus fruits was confirmed and quantified using quantitative RT-PCR (qRT-PCR). PdigCHSIII had the highest expression among the five genes by one order of magnitude, while PdigCHSII had the lowest. However, PdigCHSII was strongly induced coincident with conidial production, suggesting a role in conidiogenesis. The expression of PdigCHSI, PdigCHSIII, PdigCHSV, and PdigCHSVII was upregulated during infection of citrus fruit. PdigCHSV and PdigCHSVII coexpressed in most of the experiments carried out, and they are separated by a 1.77 kb intergenic region and arranged in opposite directions}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 20180
  AU  - Gand?a,M?nica 
  AU  - Harries,Eleonora 
  AU  - Marcos,Jose F.
  T1  - Identification and Characterization of Chitin Synthase Genes in the Postharvest Citrus Fruit Pathogen Penicillium digitatum.
  PY  - 2012
  KW  - Penicillium digitatum
  KW  - Chitin synthase
  KW  - Cell wall
  KW  - Gene expression
  KW  - qRT-PCR
  KW  - Transcriptional regulation
  UR  - http://www.sciencedirect.com/science/article/pii/S1878614612000645
  N2  - In this study, we carried out the isolation and characterization of chitin synthase genes (CHS) of the main citrus fruit postharvest pathogen Penicillium digitatum. Using distinct sets of degenerate primers designed from conserved regions of CHS genes of yeast and filamentous fungi, PCR methods and a DNA genomic library, five putative CHS genes (PdigCHSI, PdigCHSII, PdigCHSIII, PdigCHSV and PdigCHSVII) were identified, isolated, sequenced, and characterized. Phylogenetic analyses, sequence identity, and domain conservation support the annotation as CHS. A very high sequence identity and strong synteny were found with corresponding regions from the genome of Penicillium chrysogenum. Gene expression of P. digitatum CHS genes during mycelium axenic growth, under oxidative and osmotic stress conditions, and during infection of citrus fruits was confirmed and quantified using quantitative RT-PCR (qRT-PCR). PdigCHSIII had the highest expression among the five genes by one order of magnitude, while PdigCHSII had the lowest. However, PdigCHSII was strongly induced coincident with conidial production, suggesting a role in conidiogenesis. The expression of PdigCHSI, PdigCHSIII, PdigCHSV, and PdigCHSVII was upregulated during infection of citrus fruit. PdigCHSV and PdigCHSVII coexpressed in most of the experiments carried out, and they are separated by a 1.77 kb intergenic region and arranged in opposite directions
  L3  - 10.1016/j.funbio.2012.03.005
  JF  - Fungal Biology 
  VL  - 
  IS  - 
  ER  -