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Citation for Study 12145

Citation title: "Intraspecific variation of the aquatic fungus Articulospora tetracladia: an ubiquitous perspective ".

Study name: "Intraspecific variation of the aquatic fungus Articulospora tetracladia: an ubiquitous perspective ".

This study is part of submission 12145 (Status: Published).

Citation

Seena S., Duarte S., Pascoal C., & C?ssio F. 2011. Intraspecific variation of the aquatic fungus Articulospora tetracladia: an ubiquitous perspective. PLoS ONE, .

Authors

• Seena S.
• Duarte S.
• Pascoal C.
• C?ssio F.

Abstract

The worldwide-distributed aquatic fungus Articulospora tetracladia Ingold is a dominant sporulating species in streams of the Northwest Iberian Peninsula. To elucidate the genetic diversity of A. tetracladia, we analyzed isolates collected from various types of plant litter or foam in streams from North and Central Portugal and North Spain, between 2000 and 2010. Genetic diversity of these fungal populations was assessed by denaturing gradient gel electrophoresis (DGGE) fingerprints and by using ITS1-5.8S-ITS2 barcodes. Moreover, ITS1-5.8S-ITS2 barcodes of A. tetracladia reported in other parts of the world (Central Europe, UK, Canada, Japan and Malaysia) were retrieved from National Center for Biotechnology (NCBI) or National Institute of Technology and Evaluation Biological Resource Center (NBRC) to probe into genetic diversity of A. tetracladia. PCR-DGGE of ITS2 region of 50 Iberian fungal isolates distinguished eight operational taxonomic units (OTUs), which were similar to those obtained from neighbour joining tree based on ITS2 gene sequences. On the other hand, ITS1-5.8S-ITS2 barcodes of 68 fungal isolates yielded nine OTUs, but five fungal isolates were not assigned to any of these OTUs. Molecular diversity was highest for OTU-8, which included only European isolates. Two haplotypes were observed within OTU-8 and OTU-9, while only one haplotype was found within each of the remaining OTUs. Malaysia did not share haplotypes with other countries. Overall results indicate that, apart from the Malaysian genotypes, A. tetracladia genotypes were geographically widespread irrespective of sampling time, sites or substrates. Furthermore, PCR-DGGE appeared to be a rapid tool for assessing intraspecific diversity of aquatic hyphomycetes.

Keywords

Articulospora tetracladia, Genetic diversity, ITS region sequence, DGGE fingerprint

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S12145
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref20234,
  	 author = {Sahadevan  Seena and Sofia  Duarte and Cl?udia   Pascoal and Fernanda   C?ssio},
  	 title = {Intraspecific variation of the aquatic fungus Articulospora tetracladia: an ubiquitous perspective   },
  	 year = {2011},
  	 keywords = {Articulospora tetracladia, Genetic diversity, ITS region sequence, DGGE fingerprint   },
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {PLoS ONE},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {The worldwide-distributed aquatic fungus Articulospora tetracladia Ingold is a dominant sporulating species in streams of the Northwest Iberian Peninsula. To elucidate the genetic diversity of A. tetracladia, we analyzed isolates collected from various types of plant litter or foam in streams from North and Central Portugal and North Spain, between 2000 and 2010. Genetic diversity of these fungal populations was assessed by denaturing gradient gel electrophoresis (DGGE) fingerprints and by using ITS1-5.8S-ITS2 barcodes. Moreover, ITS1-5.8S-ITS2 barcodes of A. tetracladia reported in other parts of the world (Central Europe, UK, Canada, Japan and Malaysia) were retrieved from National Center for Biotechnology (NCBI) or National Institute of Technology and Evaluation Biological Resource Center (NBRC) to probe into genetic diversity of A. tetracladia. PCR-DGGE of ITS2 region of 50 Iberian fungal isolates distinguished eight operational taxonomic units (OTUs), which were similar to those obtained from neighbour joining tree based on ITS2 gene sequences.  On the other hand,  ITS1-5.8S-ITS2 barcodes of 68 fungal isolates yielded nine OTUs, but five fungal isolates were not assigned to any of these OTUs. Molecular diversity was highest for OTU-8, which included only European isolates. Two haplotypes were observed within OTU-8 and OTU-9, while only one haplotype was found within each of the remaining OTUs. Malaysia did not share haplotypes with other countries. Overall results indicate that, apart from the Malaysian genotypes, A. tetracladia genotypes were geographically widespread irrespective of sampling time, sites or substrates. Furthermore, PCR-DGGE appeared to be a rapid tool for assessing intraspecific diversity of aquatic hyphomycetes.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 20234
  AU  - Seena,Sahadevan 
  AU  - Duarte,Sofia 
  AU  - Pascoal,Cl?udia  
  AU  - C?ssio,Fernanda  
  T1  - Intraspecific variation of the aquatic fungus Articulospora tetracladia: an ubiquitous perspective   
  PY  - 2011
  KW  - Articulospora tetracladia
  KW  - Genetic diversity
  KW  - ITS region sequence
  KW  - DGGE fingerprint   
  UR  - http://dx.doi.org/
  N2  - The worldwide-distributed aquatic fungus Articulospora tetracladia Ingold is a dominant sporulating species in streams of the Northwest Iberian Peninsula. To elucidate the genetic diversity of A. tetracladia, we analyzed isolates collected from various types of plant litter or foam in streams from North and Central Portugal and North Spain, between 2000 and 2010. Genetic diversity of these fungal populations was assessed by denaturing gradient gel electrophoresis (DGGE) fingerprints and by using ITS1-5.8S-ITS2 barcodes. Moreover, ITS1-5.8S-ITS2 barcodes of A. tetracladia reported in other parts of the world (Central Europe, UK, Canada, Japan and Malaysia) were retrieved from National Center for Biotechnology (NCBI) or National Institute of Technology and Evaluation Biological Resource Center (NBRC) to probe into genetic diversity of A. tetracladia. PCR-DGGE of ITS2 region of 50 Iberian fungal isolates distinguished eight operational taxonomic units (OTUs), which were similar to those obtained from neighbour joining tree based on ITS2 gene sequences.  On the other hand,  ITS1-5.8S-ITS2 barcodes of 68 fungal isolates yielded nine OTUs, but five fungal isolates were not assigned to any of these OTUs. Molecular diversity was highest for OTU-8, which included only European isolates. Two haplotypes were observed within OTU-8 and OTU-9, while only one haplotype was found within each of the remaining OTUs. Malaysia did not share haplotypes with other countries. Overall results indicate that, apart from the Malaysian genotypes, A. tetracladia genotypes were geographically widespread irrespective of sampling time, sites or substrates. Furthermore, PCR-DGGE appeared to be a rapid tool for assessing intraspecific diversity of aquatic hyphomycetes.
  L3  - 
  JF  - PLoS ONE
  VL  - 
  IS  - 
  ER  -