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Citation for Study 12165

Citation title: "A Phylogenetic Analysis of Greek Isolates of Aspergillus Species Based on Morphology and Nuclear and Mitochondrial Gene Sequences".

Study name: "A Phylogenetic Analysis of Greek Isolates of Aspergillus Species Based on Morphology and Nuclear and Mitochondrial Gene Sequences".

This study is part of submission 12165 (Status: Published).

Citation

Krimitzas A., Pyrri I., Kouvelis V.N., Kapsanaki-gotsi E., & Typas M.A. 2013. A Phylogenetic Analysis of Greek Isolates of Aspergillus Species Based on Morphology and Nuclear and Mitochondrial Gene Sequences. BioMed Research International, 2013(260395): 18.

Authors

• Krimitzas A.
• Pyrri I.
• Kouvelis V.N. (submitter) +302107274488
• Kapsanaki-gotsi E.
• Typas M.A.

Abstract

Aspergillus species originating from Greece were examined by morphological and molecular criteria to explore the diversity of this genus. The phylogenetic relationships of these species were determined using sequences from the ITS and IGS region of the nuclear rRNA gene complex, two nuclear genes [β-tubulin (benA) and RNA polymerase II second largest subunit (rpb2)] and two mitochondrial genes [small rRNA subunit (rns) and cytochrome oxidase subunit I (cox1)] and, where available, related sequences from databases. The morphological characters of the anamorphs and teleomorphs, and the single gene phylogenetic trees, differentiated and placed the species examined in the well-supported sections of Aenei, Aspergillus, Bispori, Candidi, Circumdati, Clavati, Cremei, Flavi, Flavipedes, Fumigati, Nidulantes, Nigri, Restricti, Terrei, Usti and Zonati, with few uncertainties. The combined use of the three commonly employed nuclear genes (benA, rpb2 and ITS), the IGS region, and two less often used mitochondrial gene sequences (rns and cox1) as a single unit, resolved several taxonomic ambiguities. A phylogenetic tree was inferred using Neighbour-Joining, Maximum Parsimony and Bayesian methods. The strains examined formed seven well-supported clades within the genus Aspergillus. Altogether, the concatenated nuclear and mitochondrial sequences offer additional tools for an improved understanding of phylogenetic relationships within this genus.

Keywords

Aspergillus; nuclear genes; mitochondrial genes; multi-locus phylogeny; anamorphs; teleomorphs

External links

• DOI: 10.1155/2013/260395
• Other URL: http://www.hindawi.com/journals/bmri/2013/260395/

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S12165
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref20250,
  	 author = {Antonis  Krimitzas and Ioanna  Pyrri and Vassili N Kouvelis and Evangelia  Kapsanaki-Gotsi and Milton A Typas},
  	 title = {A Phylogenetic Analysis of Greek Isolates of Aspergillus Species Based on Morphology and Nuclear and Mitochondrial Gene Sequences},
  	 year = {2013},
  	 keywords = {Aspergillus; nuclear genes; mitochondrial genes; multi-locus phylogeny; anamorphs; teleomorphs},
  	 doi = {10.1155/2013/260395},
  	 url = {http://www.hindawi.com/journals/bmri/2013/260395/},
  	 pmid = {},
  	 journal = { BioMed Research International},
  	 volume = {2013},
  	 number = {260395},
  	 pages = {18},
  	 abstract = {Aspergillus species originating from Greece were examined by morphological and molecular criteria to explore the diversity of this genus. The phylogenetic relationships of these species were determined using sequences from the ITS and IGS region of the nuclear rRNA gene complex, two nuclear genes [β-tubulin (benA) and RNA polymerase II second largest subunit (rpb2)] and two mitochondrial genes [small rRNA subunit (rns) and cytochrome oxidase subunit I (cox1)] and, where available, related sequences from databases. The morphological characters of the anamorphs and teleomorphs, and the single gene phylogenetic trees, differentiated and placed the species examined in the well-supported sections of Aenei, Aspergillus, Bispori, Candidi, Circumdati, Clavati, Cremei, Flavi, Flavipedes, Fumigati, Nidulantes, Nigri, Restricti, Terrei, Usti and Zonati, with few uncertainties. The combined use of the three commonly employed nuclear genes (benA, rpb2 and ITS), the IGS region, and two less often used mitochondrial gene sequences (rns and cox1) as a single unit, resolved several taxonomic ambiguities. A phylogenetic tree was inferred using Neighbour-Joining, Maximum Parsimony and Bayesian methods. The strains examined formed seven well-supported clades within the genus Aspergillus. Altogether, the concatenated nuclear and mitochondrial sequences offer additional tools for an improved understanding
  of phylogenetic relationships within this genus.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 20250
  AU  - Krimitzas,Antonis 
  AU  - Pyrri,Ioanna 
  AU  - Kouvelis,Vassili N
  AU  - Kapsanaki-Gotsi,Evangelia 
  AU  - Typas,Milton A
  T1  - A Phylogenetic Analysis of Greek Isolates of Aspergillus Species Based on Morphology and Nuclear and Mitochondrial Gene Sequences
  PY  - 2013
  KW  - Aspergillus; nuclear genes; mitochondrial genes; multi-locus phylogeny; anamorphs; teleomorphs
  UR  - http://www.hindawi.com/journals/bmri/2013/260395/
  N2  - Aspergillus species originating from Greece were examined by morphological and molecular criteria to explore the diversity of this genus. The phylogenetic relationships of these species were determined using sequences from the ITS and IGS region of the nuclear rRNA gene complex, two nuclear genes [β-tubulin (benA) and RNA polymerase II second largest subunit (rpb2)] and two mitochondrial genes [small rRNA subunit (rns) and cytochrome oxidase subunit I (cox1)] and, where available, related sequences from databases. The morphological characters of the anamorphs and teleomorphs, and the single gene phylogenetic trees, differentiated and placed the species examined in the well-supported sections of Aenei, Aspergillus, Bispori, Candidi, Circumdati, Clavati, Cremei, Flavi, Flavipedes, Fumigati, Nidulantes, Nigri, Restricti, Terrei, Usti and Zonati, with few uncertainties. The combined use of the three commonly employed nuclear genes (benA, rpb2 and ITS), the IGS region, and two less often used mitochondrial gene sequences (rns and cox1) as a single unit, resolved several taxonomic ambiguities. A phylogenetic tree was inferred using Neighbour-Joining, Maximum Parsimony and Bayesian methods. The strains examined formed seven well-supported clades within the genus Aspergillus. Altogether, the concatenated nuclear and mitochondrial sequences offer additional tools for an improved understanding
  of phylogenetic relationships within this genus.
  L3  - 10.1155/2013/260395
  JF  -  BioMed Research International
  VL  - 2013
  IS  - 260395
  ER  -