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Citation for Study 12458

Citation title: "Characterization and gene expression analysis of the cir multi-gene family of Plasmodium chabaudi chabaudi (AS)".

Study name: "Characterization and gene expression analysis of the cir multi-gene family of Plasmodium chabaudi chabaudi (AS)".

This study is part of submission 12458 (Status: Published).

Citation

Lawton J., Brugat T., Yam X., Reid A.J., B?hme U., Otto T.D., Pain A., Jackson A., Berriman M., Cunningham D., Preiser P.R., & Langhorne J. 2012. Characterization and gene expression analysis of the cir multi-gene family of Plasmodium chabaudi chabaudi (AS). BMC Genomics, 13(125 ).

Authors

• Lawton J. (submitter) +442088162409
• Brugat T.
• Yam X.
• Reid A.J.
• B?hme U.
• Otto T.D.
• Pain A.
• Jackson A.
• Berriman M.
• Cunningham D.
• Preiser P.R.
• Langhorne J.

Abstract

Background: The pir genes comprise the largest multi-gene family in Plasmodium, with members found in P. vivax, P. knowlesi and the rodent malaria species. Despite comprising up to 5% of the genome, little is known about the functions of the proteins encoded by pir genes. P. chabaudi causes chronic infection in mice, which may be due to antigenic variation. In this model, pir genes are called cirs and may be involved in this mechanism, allowing evasion of host immune responses. In order to fully understand the role(s) of CIR proteins during P. chabaudi infection, a detailed characterization of the cir gene family was required. Results: The cir repertoire was annotated and a detailed bioinformatic characterization of the encoded CIR proteins was performed. Two major sub-families were identified, which have been named A and B. Members of each sub-family displayed different amino acid motifs, and were thus predicted to have undergone functional divergence. In addition, the expression of the entire cir repertoire was analyzed via RNA sequencing and microarray. Up to 40% of the cir gene repertoire was expressed in the parasite population during infection, and dominant cir transcripts could be identified. In addition, some differences were observed in the pattern of expression between the cir subgroups at the peak of P. chabaudi infection. Finally, specific cir genes were expressed at different time points during asexual blood stages. Conclusions: In conclusion, the large number of cir genes and their expression throughout the intra-erythrocytic cycle of development indicates that CIR proteins are likely to be important for parasite survival. In particular, the detection of dominant cir transcripts at the peak of P. chabaudi infection supports the idea that CIR proteins are expressed, and could perform important functions in the biology of this parasite.

Keywords

Multi-gene family, Malaria, Rodent model

External links

• DOI: 10.1186/1471-2164-13-125
• PMID: 224588
• Other URL: http://www.biomedcentral.com/1471-2164/13/125/abstract

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S12458
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref20506,
  	 author = {Jenni  Lawton and Thibaut  Brugat and Xue Yan  Yam and Adam James Reid and Ulrike  B?hme and Thomas Dan Otto and Arnab  Pain and Andrew  Jackson and Matthew  Berriman and Deirdre  Cunningham and Peter Rainer Preiser and Jean  Langhorne},
  	 title = {Characterization and gene expression analysis of the cir multi-gene family of Plasmodium chabaudi chabaudi (AS)},
  	 year = {2012},
  	 keywords = {Multi-gene family, Malaria, Rodent model},
  	 doi = {10.1186/1471-2164-13-125},
  	 url = {http://www.biomedcentral.com/1471-2164/13/125/abstract},
  	 pmid = {224588},
  	 journal = {BMC Genomics},
  	 volume = {13},
  	 number = {125 },
  	 pages = {},
  	 abstract = {Background: The pir genes comprise the largest multi-gene family in Plasmodium,
  with members found in P. vivax, P. knowlesi and the rodent malaria species. Despite
  comprising up to 5% of the genome, little is known about the functions of the proteins
  encoded by pir genes. P. chabaudi causes chronic infection in mice, which may be
  due to antigenic variation. In this model, pir genes are called cirs and may be
  involved in this mechanism, allowing evasion of host immune responses. In order to
  fully understand the role(s) of CIR proteins during P. chabaudi infection, a detailed
  characterization of the cir gene family was required.
  Results: The cir repertoire was annotated and a detailed bioinformatic
  characterization of the encoded CIR proteins was performed. Two major sub-families
  were identified, which have been named A and B. Members of each sub-family
  displayed different amino acid motifs, and were thus predicted to have undergone
  functional divergence. In addition, the expression of the entire cir repertoire was
  analyzed via RNA sequencing and microarray. Up to 40% of the cir gene repertoire
  was expressed in the parasite population during infection, and dominant cir transcripts
  could be identified. In addition, some differences were observed in the pattern of
  expression between the cir subgroups at the peak of P. chabaudi infection. Finally,
  specific cir genes were expressed at different time points during asexual blood stages.
  Conclusions: In conclusion, the large number of cir genes and their expression
  throughout the intra-erythrocytic cycle of development indicates that CIR proteins are
  likely to be important for parasite survival. In particular, the detection of dominant cir
  transcripts at the peak of P. chabaudi infection supports the idea that CIR proteins are
  expressed, and could perform important functions in the biology of this parasite.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 20506
  AU  - Lawton,Jenni 
  AU  - Brugat,Thibaut 
  AU  - Yam,Xue Yan 
  AU  - Reid,Adam James
  AU  - B?hme,Ulrike 
  AU  - Otto,Thomas Dan
  AU  - Pain,Arnab 
  AU  - Jackson,Andrew 
  AU  - Berriman,Matthew 
  AU  - Cunningham,Deirdre 
  AU  - Preiser,Peter Rainer
  AU  - Langhorne,Jean 
  T1  - Characterization and gene expression analysis of the cir multi-gene family of Plasmodium chabaudi chabaudi (AS)
  PY  - 2012
  KW  - Multi-gene family
  KW  - Malaria
  KW  - Rodent model
  UR  - http://www.biomedcentral.com/1471-2164/13/125/abstract
  N2  - Background: The pir genes comprise the largest multi-gene family in Plasmodium,
  with members found in P. vivax, P. knowlesi and the rodent malaria species. Despite
  comprising up to 5% of the genome, little is known about the functions of the proteins
  encoded by pir genes. P. chabaudi causes chronic infection in mice, which may be
  due to antigenic variation. In this model, pir genes are called cirs and may be
  involved in this mechanism, allowing evasion of host immune responses. In order to
  fully understand the role(s) of CIR proteins during P. chabaudi infection, a detailed
  characterization of the cir gene family was required.
  Results: The cir repertoire was annotated and a detailed bioinformatic
  characterization of the encoded CIR proteins was performed. Two major sub-families
  were identified, which have been named A and B. Members of each sub-family
  displayed different amino acid motifs, and were thus predicted to have undergone
  functional divergence. In addition, the expression of the entire cir repertoire was
  analyzed via RNA sequencing and microarray. Up to 40% of the cir gene repertoire
  was expressed in the parasite population during infection, and dominant cir transcripts
  could be identified. In addition, some differences were observed in the pattern of
  expression between the cir subgroups at the peak of P. chabaudi infection. Finally,
  specific cir genes were expressed at different time points during asexual blood stages.
  Conclusions: In conclusion, the large number of cir genes and their expression
  throughout the intra-erythrocytic cycle of development indicates that CIR proteins are
  likely to be important for parasite survival. In particular, the detection of dominant cir
  transcripts at the peak of P. chabaudi infection supports the idea that CIR proteins are
  expressed, and could perform important functions in the biology of this parasite.
  L3  - 10.1186/1471-2164-13-125
  JF  - BMC Genomics
  VL  - 13
  IS  - 125 
  ER  -