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Citation for Study 13914

Citation title: "Phylogenetic status and pathogenicity of Metarhizium majus isolated from a fruit beetle larva in Japan".

Study name: "Phylogenetic status and pathogenicity of Metarhizium majus isolated from a fruit beetle larva in Japan".

This study is part of submission 13914 (Status: Published).

Citation

Nishi O., Iiyama K., Yasunaga-aoki C., & Shimizu S. 2015. Phylogenetic status and pathogenicity of Metarhizium majus isolated from a fruit beetle larva in Japan. Mycological Progress, 14: 58.

Authors

• Nishi O.
• Iiyama K.
• Yasunaga-aoki C.
• Shimizu S.

Abstract

Some isolates of Metarhizium anisopliae sensu lato with large conidia (MALC), which was formerly known as M. anisopliae var. majus, from a scarabaeid host, such as isolates from the coconut rhinoceros beetle (Oryctes spp.), have a narrow host range and are particularly adapted to their original host. Despite recent taxonomic revisions of the genus Metarhizium on the basis of DNA sequences, variations in host preferences of MALC have not been linked to DNA sequence variations. This study focused on the phylogenetic status and pathogenicity of MALC isolated from the fruit bee- tle larva Protaetia orientalis submarumorea (Pos) in Japan to investigate the relationship between virulence and nucleotide sequence variation among MALC. On the basis of the results of the phylogenetic analyses of DNA sequences from five loci, we identified an isolate from Pos (Hn1) as M. majus, which comprised many isolates with large conidia from scarabaeid insects. In the virulence assay of genetically di- verse Metarhizium isolates, only Hn1 and its most closely related isolates from soil showed pathogenicity to Pos; how- ever, these Pos-pathogenic isolates showed weak virulence against silkworms. In the analysis of the intergenic spacer region of rDNA, the Pos-pathogenic isolates displayed unique sequence variation and were clearly distinguished from close- ly related lineages, including isolates from Oryctes sp., which formed a separate monophyletic group. These results indicate that the Pos-pathogenic isolates from Japan are particularly adapted to Pos and can be genetically distinguished from M. majus isolates from different scarabaeid insects.

Keywords

Metarhizium majus, fruit beetle, Scarabaeidae, phylogenetic analysis, virulence

External links

• DOI: 10.1007/s11557-015-1082-7

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S13914
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref21754,
  	 author = {Oumi  NISHI and Kazuhiro  IIYAMA and Chisa  YASUNAGA-AOKI and Susumu  SHIMIZU},
  	 title = {Phylogenetic status and pathogenicity of Metarhizium majus isolated from a fruit beetle larva in Japan},
  	 year = {2015},
  	 keywords = {Metarhizium majus, fruit beetle, Scarabaeidae, phylogenetic analysis, virulence},
  	 doi = {10.1007/s11557-015-1082-7},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Mycological Progress},
  	 volume = {14},
  	 number = {},
  	 pages = {58},
  	 abstract = {Some isolates of Metarhizium anisopliae sensu lato with large conidia (MALC), which was formerly known as M. anisopliae var. majus, from a scarabaeid host, such as isolates from the coconut rhinoceros beetle (Oryctes spp.), have a narrow host range and are particularly adapted to their original host. Despite recent taxonomic revisions of the genus Metarhizium on the basis of DNA sequences, variations in host preferences of MALC have not been linked to DNA sequence variations. This study focused on the phylogenetic status and pathogenicity of MALC isolated from the fruit bee- tle larva Protaetia orientalis submarumorea (Pos) in Japan to investigate the relationship between virulence and nucleotide sequence variation among MALC. On the basis of the results of the phylogenetic analyses of DNA sequences from five loci, we identified an isolate from Pos (Hn1) as M. majus, which comprised many isolates with large conidia from scarabaeid insects. In the virulence assay of genetically di- verse Metarhizium isolates, only Hn1 and its most closely related isolates from soil showed pathogenicity to Pos; how- ever, these Pos-pathogenic isolates showed weak virulence against silkworms. In the analysis of the intergenic spacer region of rDNA, the Pos-pathogenic isolates displayed unique sequence variation and were clearly distinguished from close- ly related lineages, including isolates from Oryctes sp., which formed a separate monophyletic group. These results indicate that the Pos-pathogenic isolates from Japan are particularly adapted to Pos and can be genetically distinguished from M. majus isolates from different scarabaeid insects.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 21754
  AU  - NISHI,Oumi 
  AU  - IIYAMA,Kazuhiro 
  AU  - YASUNAGA-AOKI,Chisa 
  AU  - SHIMIZU,Susumu 
  T1  - Phylogenetic status and pathogenicity of Metarhizium majus isolated from a fruit beetle larva in Japan
  PY  - 2015
  KW  - Metarhizium majus
  KW  - fruit beetle
  KW  - Scarabaeidae
  KW  - phylogenetic analysis
  KW  - virulence
  UR  - http://dx.doi.org/10.1007/s11557-015-1082-7
  N2  - Some isolates of Metarhizium anisopliae sensu lato with large conidia (MALC), which was formerly known as M. anisopliae var. majus, from a scarabaeid host, such as isolates from the coconut rhinoceros beetle (Oryctes spp.), have a narrow host range and are particularly adapted to their original host. Despite recent taxonomic revisions of the genus Metarhizium on the basis of DNA sequences, variations in host preferences of MALC have not been linked to DNA sequence variations. This study focused on the phylogenetic status and pathogenicity of MALC isolated from the fruit bee- tle larva Protaetia orientalis submarumorea (Pos) in Japan to investigate the relationship between virulence and nucleotide sequence variation among MALC. On the basis of the results of the phylogenetic analyses of DNA sequences from five loci, we identified an isolate from Pos (Hn1) as M. majus, which comprised many isolates with large conidia from scarabaeid insects. In the virulence assay of genetically di- verse Metarhizium isolates, only Hn1 and its most closely related isolates from soil showed pathogenicity to Pos; how- ever, these Pos-pathogenic isolates showed weak virulence against silkworms. In the analysis of the intergenic spacer region of rDNA, the Pos-pathogenic isolates displayed unique sequence variation and were clearly distinguished from close- ly related lineages, including isolates from Oryctes sp., which formed a separate monophyletic group. These results indicate that the Pos-pathogenic isolates from Japan are particularly adapted to Pos and can be genetically distinguished from M. majus isolates from different scarabaeid insects.
  L3  - 10.1007/s11557-015-1082-7
  JF  - Mycological Progress
  VL  - 14
  IS  - 
  ER  -