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Citation for Study 15139

Citation title: "Phylogenetic analysis of faecal microbiota from captive cheetahs reveals underrepresentation of Bacteroidetes and Bifidobacteriaceae ".

Study name: "Phylogenetic analysis of faecal microbiota from captive cheetahs reveals underrepresentation of Bacteroidetes and Bifidobacteriaceae ".

This study is part of submission 15139 (Status: Published).

Citation

Becker A.A., Hesta M., Hollants J., Janssens G.P., & Huys G. 2014. Phylogenetic analysis of faecal microbiota from captive cheetahs reveals underrepresentation of Bacteroidetes and Bifidobacteriaceae. BMC Microbiology, 14(43).

Authors

• Becker A.A. (submitter)
• Hesta M.
• Hollants J.
• Janssens G.P.
• Huys G.

Abstract

Background: Imbalanced feeding regimes may initiate gastrointestinal and metabolic diseases in endangered felids kept in captivity such as cheetahs. Given the crucial role of the host?s intestinal microbiota in feed fermentation and health maintenance, a better understanding of the cheetah?s intestinal ecosystem is essential for improvement of current feeding strategies. We determined the phylogenetic diversity of the faecal microbiota of the only two cheetahs housed in an EAZA associated zoo in Flanders, Belgium, to gain first insights in the relative distribution, identity and potential role of the major community members. Results: Taxonomic analysis of 16S rRNA gene clone libraries (702 clones) revealed a microbiota dominated by Firmicutes (94.7%), followed by a minority of Actinobacteria (4.3%), Proteobacteria (0.4%) and Fusobacteria (0.6%). In the Firmicutes, the majority of the phylotypes within the Clostridiales were assigned to Clostridium clusters XIVa (43%), XI (38%) and I (13%). Members of the Bacteroidetes phylum and Bifidobacteriaceae, two groups that can positively contribute in maintaining intestinal homeostasis, were absent in the clone libraries and detected in only marginal to low levels in real-time PCR analyses. Conclusions: This marked underrepresentation is in contrast to data previously reported in domestic cats where Bacteroidetes and Bifidobacteriaceae are common residents of the faecal microbiota. Next to methodological differences, these findings may also reflect the apparent differences in dietary habits of both felid species. Thus, our results question the role of the domestic cat as the best available model for nutritional intervention studies in endangered exotic felids.

Keywords

bacterial community sequencing, exotic felids, wildlife conservation, zoo nutrition

External links

• DOI: 10.1186/1471-2180-14-43
• Other URL: http://www.biomedcentral.com/1471-2180/14/43/abstract

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S15139
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref22749,
  	 author = {Anne AMJ Becker and Myriam  Hesta and Joke  Hollants and Geert PJ Janssens and Geert  Huys},
  	 title = {Phylogenetic analysis of faecal microbiota from captive cheetahs reveals underrepresentation of Bacteroidetes and Bifidobacteriaceae },
  	 year = {2014},
  	 keywords = {bacterial community sequencing, exotic felids, wildlife conservation, zoo nutrition},
  	 doi = {10.1186/1471-2180-14-43},
  	 url = {http://www.biomedcentral.com/1471-2180/14/43/abstract},
  	 pmid = {},
  	 journal = {BMC Microbiology},
  	 volume = {14},
  	 number = {43},
  	 pages = {},
  	 abstract = {Background: Imbalanced feeding regimes may initiate gastrointestinal and metabolic diseases in endangered felids kept in captivity such as cheetahs. Given the crucial role of the host?s intestinal microbiota in feed fermentation and health maintenance, a better understanding of the cheetah?s intestinal ecosystem is essential for improvement of current feeding strategies. We determined the phylogenetic diversity of the faecal microbiota of the only two cheetahs housed in an EAZA associated zoo in Flanders, Belgium, to gain first insights in the relative distribution, identity and potential role of the major community members.
  Results: Taxonomic analysis of 16S rRNA gene clone libraries (702 clones) revealed a microbiota dominated by Firmicutes (94.7%), followed by a minority of Actinobacteria (4.3%), Proteobacteria (0.4%) and Fusobacteria (0.6%). In the Firmicutes, the majority of the phylotypes within the Clostridiales were assigned to Clostridium clusters XIVa (43%), XI (38%) and I (13%). Members of the Bacteroidetes phylum and Bifidobacteriaceae, two groups that can positively contribute in maintaining intestinal homeostasis, were absent in the clone libraries and detected in only marginal to low levels in real-time PCR analyses.
  Conclusions: This marked underrepresentation is in contrast to data previously reported in domestic cats where Bacteroidetes and Bifidobacteriaceae are common residents of the faecal microbiota. Next to methodological differences, these findings may also reflect the apparent differences in dietary habits of both felid species. Thus, our results question the role of the domestic cat as the best available model for nutritional intervention studies in endangered exotic felids.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 22749
  AU  - Becker,Anne AMJ
  AU  - Hesta,Myriam 
  AU  - Hollants,Joke 
  AU  - Janssens,Geert PJ
  AU  - Huys,Geert 
  T1  - Phylogenetic analysis of faecal microbiota from captive cheetahs reveals underrepresentation of Bacteroidetes and Bifidobacteriaceae 
  PY  - 2014
  KW  - bacterial community sequencing
  KW  - exotic felids
  KW  - wildlife conservation
  KW  - zoo nutrition
  UR  - http://www.biomedcentral.com/1471-2180/14/43/abstract
  N2  - Background: Imbalanced feeding regimes may initiate gastrointestinal and metabolic diseases in endangered felids kept in captivity such as cheetahs. Given the crucial role of the host?s intestinal microbiota in feed fermentation and health maintenance, a better understanding of the cheetah?s intestinal ecosystem is essential for improvement of current feeding strategies. We determined the phylogenetic diversity of the faecal microbiota of the only two cheetahs housed in an EAZA associated zoo in Flanders, Belgium, to gain first insights in the relative distribution, identity and potential role of the major community members.
  Results: Taxonomic analysis of 16S rRNA gene clone libraries (702 clones) revealed a microbiota dominated by Firmicutes (94.7%), followed by a minority of Actinobacteria (4.3%), Proteobacteria (0.4%) and Fusobacteria (0.6%). In the Firmicutes, the majority of the phylotypes within the Clostridiales were assigned to Clostridium clusters XIVa (43%), XI (38%) and I (13%). Members of the Bacteroidetes phylum and Bifidobacteriaceae, two groups that can positively contribute in maintaining intestinal homeostasis, were absent in the clone libraries and detected in only marginal to low levels in real-time PCR analyses.
  Conclusions: This marked underrepresentation is in contrast to data previously reported in domestic cats where Bacteroidetes and Bifidobacteriaceae are common residents of the faecal microbiota. Next to methodological differences, these findings may also reflect the apparent differences in dietary habits of both felid species. Thus, our results question the role of the domestic cat as the best available model for nutritional intervention studies in endangered exotic felids.
  L3  - 10.1186/1471-2180-14-43
  JF  - BMC Microbiology
  VL  - 14
  IS  - 43
  ER  -