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Citation for Study 15193

Citation title: "Comparative genomics of unintrogressed Campylobacter coli clades 2 and 3".

Study name: "Comparative genomics of unintrogressed Campylobacter coli clades 2 and 3".

This study is part of submission 15193 (Status: Published).

Citation

De haan C.P., Culebro A., Schott T., Revez J., Schweda E., H?nninen M., & Rossi M. 2014. Comparative genomics of unintrogressed Campylobacter coli clades 2 and 3. BMC Genomics, .

Authors

• De haan C.P.
• Culebro A.
• Schott T.
• Revez J.
• Schweda E.
• H?nninen M.
• Rossi M. (submitter) 00358443709933

Abstract

Background. Campylobacter jejuni and C. coli share a multitude of risk factors associated with human gastrointestinal disease, yet their phylogeny differs significantly. C. jejuni are scattered into several lineages, with no apparent linkage, whereas C. coli cluster into three distinct phylogenetic groups (clades) of which clade 1 showed extensive genome-wide introgression with C. jejuni, yet the other two clades (2 and 3) show less than 2% of C. jejuni ancestry. We identified a C. coli strain (76339) with four novel multilocus sequence type alleles (ST-5088) and the capability to express gamma-glutamyltranspeptidase (GGT); an accessory feature in C. jejuni. Our aim was to characterize unintrogressed C. coli clades 2 and 3, using whole genome sequence analysis, to investigate the impact of horizontal gene transfer in shaping accessory and core genes pool in unintrogressed C. coli. Results. Here, we present the first fully closed C. coli clade 3 genome. We studied the phylogenomics of the reference strain 76339, which revealed that strain 76339 belonged to clade 3 of unintrogressed C. coli. In addition, a more extensive respiratory metabolism among unintrogressed C. coli strains was found. We describe putative virulence factors, such as serine proteases and an active sialyltransferase in the lipooligosaccharide locus and we propose a unique scenario for the evolution of Campylobacter ggt. Conclusions. We propose a new insight into the evolution of the accessory genome of both C. coli and C. jejuni. Also, in silico analysis of the gene content revealed that C. coli clades 2 and 3 may be more pathogenic to humans than clade 1 and may currently be underreported in human infections due to niche separation.

Keywords

Campylobacter coli, comparative genomics, phylogeny, gamma glutamyltranspeptidase, sialyltransferase

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S15193
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref22742,
  	 author = {C PA De Haan and Alejandra  Culebro and Thomas  Schott and Joana  Revez and Elke  Schweda and Marja-Liisa  H?nninen and Mirko  Rossi},
  	 title = {Comparative genomics of unintrogressed Campylobacter coli clades 2 and 3},
  	 year = {2014},
  	 keywords = {Campylobacter coli, comparative genomics, phylogeny, gamma glutamyltranspeptidase, sialyltransferase},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {BMC Genomics},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Background. Campylobacter jejuni and C. coli share a multitude of risk factors associated with human gastrointestinal disease, yet their phylogeny differs significantly. C. jejuni are scattered into several lineages, with no apparent linkage, whereas C. coli cluster into three distinct phylogenetic groups (clades) of which clade 1 showed extensive genome-wide introgression with C. jejuni, yet the other two clades (2 and 3) show less than 2% of C. jejuni ancestry. We identified a C. coli strain (76339) with four novel multilocus sequence type alleles (ST-5088) and the capability to express gamma-glutamyltranspeptidase (GGT); an accessory feature in C. jejuni.  Our aim was to characterize unintrogressed C. coli clades 2 and 3, using whole genome sequence analysis, to investigate the impact of horizontal gene transfer in shaping accessory and core genes pool in unintrogressed C. coli.
  Results. Here, we present the first fully closed C. coli clade 3 genome. We studied the phylogenomics of the reference strain 76339, which revealed that strain 76339 belonged to clade 3 of unintrogressed C. coli. In addition, a more extensive respiratory metabolism among unintrogressed C. coli strains was found. We describe putative virulence factors, such as serine proteases and an active sialyltransferase in the lipooligosaccharide locus and we propose a unique scenario for the evolution of Campylobacter ggt.
  Conclusions. We propose a new insight into the evolution of the accessory genome of both C. coli and C. jejuni. Also, in silico analysis of the gene content revealed that C. coli clades 2 and 3 may be more pathogenic to humans than clade 1 and may currently be underreported in human infections due to niche separation.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 22742
  AU  - De Haan,C PA
  AU  - Culebro,Alejandra 
  AU  - Schott,Thomas 
  AU  - Revez,Joana 
  AU  - Schweda,Elke 
  AU  - H?nninen,Marja-Liisa 
  AU  - Rossi,Mirko 
  T1  - Comparative genomics of unintrogressed Campylobacter coli clades 2 and 3
  PY  - 2014
  KW  - Campylobacter coli
  KW  - comparative genomics
  KW  - phylogeny
  KW  - gamma glutamyltranspeptidase
  KW  - sialyltransferase
  UR  - http://dx.doi.org/
  N2  - Background. Campylobacter jejuni and C. coli share a multitude of risk factors associated with human gastrointestinal disease, yet their phylogeny differs significantly. C. jejuni are scattered into several lineages, with no apparent linkage, whereas C. coli cluster into three distinct phylogenetic groups (clades) of which clade 1 showed extensive genome-wide introgression with C. jejuni, yet the other two clades (2 and 3) show less than 2% of C. jejuni ancestry. We identified a C. coli strain (76339) with four novel multilocus sequence type alleles (ST-5088) and the capability to express gamma-glutamyltranspeptidase (GGT); an accessory feature in C. jejuni.  Our aim was to characterize unintrogressed C. coli clades 2 and 3, using whole genome sequence analysis, to investigate the impact of horizontal gene transfer in shaping accessory and core genes pool in unintrogressed C. coli.
  Results. Here, we present the first fully closed C. coli clade 3 genome. We studied the phylogenomics of the reference strain 76339, which revealed that strain 76339 belonged to clade 3 of unintrogressed C. coli. In addition, a more extensive respiratory metabolism among unintrogressed C. coli strains was found. We describe putative virulence factors, such as serine proteases and an active sialyltransferase in the lipooligosaccharide locus and we propose a unique scenario for the evolution of Campylobacter ggt.
  Conclusions. We propose a new insight into the evolution of the accessory genome of both C. coli and C. jejuni. Also, in silico analysis of the gene content revealed that C. coli clades 2 and 3 may be more pathogenic to humans than clade 1 and may currently be underreported in human infections due to niche separation.
  L3  - 
  JF  - BMC Genomics
  VL  - 
  IS  - 
  ER  -