@ARTICLE{TreeBASE2Ref23177,
author = {Jeffrey J Shaw and Daniel J Spakowicz and Rahul S Dalal and Jared H Davis and Nina A Lehr and Brian F Dunican and Esteban A Orellana and Alexandra Narv?ez-Trujillo and Scott A Strobel},
title = {Biosynthesis and genomic analysis of medium-chain hydrocarbon production by the endophytic fungal isolate Nigrograna mackinonnii (E5202H)},
year = {2014},
keywords = {Endophyte, natural product, Volatile Organic Compound, polyene, medium-chain hydrocarbon, biofuel, polyketide synthase},
doi = {},
url = {},
pmid = {},
journal = {Applied Microbiology and Biotechnology},
volume = {},
number = {},
pages = {},
abstract = {An endophytic fungus was isolated that produces a series of volatile natural products, including terpenes and odd chain polyenes. Phylogenetic analysis of the isolate using five loci suggests that it is closely related to Nigrograna mackinnonii CBS 674.75. The main component of the polyene series was purified and identified as (3E,5E,7E)-nona-1,3,5,7-tetraene (NTE), a novel natural product. Non-oxygenated hydrocarbons of this chain length are uncommon and desirable as gasoline-surrogate biofuels. The biosynthetic pathway for NTE production was explored using metabolic labeling and GCMS. Two-carbon incorporation 13C acetate suggests that it is derived from a polyketide synthase (PKS) followed by decarboxylation. There are several known mechanisms for such decarboxylation, though none have been discovered in fungi. Towards identifying the PKS responsible for the production of NTE, the genome of N. mackinnonii E5202H (ATCC SD-6839) was sequenced and assembled. Of the 32 PKSs present in the genome, 17 are predicted to contain sufficient domains for the production of NTE. These results exemplify the capacity of endophytic fungi to produce novel natural products that may have many uses, such as biologically derived fuels and commodity chemicals.}
}
Citation for Study 15775
Citation title:
"Biosynthesis and genomic analysis of medium-chain hydrocarbon production by the endophytic fungal isolate Nigrograna mackinonnii (E5202H)".
Study name:
"Biosynthesis and genomic analysis of medium-chain hydrocarbon production by the endophytic fungal isolate Nigrograna mackinonnii (E5202H)".
This study is part of submission 15775
(Status: Published).
Citation
Shaw J.J., Spakowicz D.J., Dalal R.S., Davis J.H., Lehr N.A., Dunican B.F., Orellana E.A., Narv?ez-trujillo A., & Strobel S.A. 2014. Biosynthesis and genomic analysis of medium-chain hydrocarbon production by the endophytic fungal isolate Nigrograna mackinonnii (E5202H). Applied Microbiology and Biotechnology, .
Authors
-
Shaw J.J.
-
Spakowicz D.J.
(submitter)
203-432-8949
-
Dalal R.S.
-
Davis J.H.
-
Lehr N.A.
-
Dunican B.F.
-
Orellana E.A.
-
Narv?ez-trujillo A.
-
Strobel S.A.
Abstract
An endophytic fungus was isolated that produces a series of volatile natural products, including terpenes and odd chain polyenes. Phylogenetic analysis of the isolate using five loci suggests that it is closely related to Nigrograna mackinnonii CBS 674.75. The main component of the polyene series was purified and identified as (3E,5E,7E)-nona-1,3,5,7-tetraene (NTE), a novel natural product. Non-oxygenated hydrocarbons of this chain length are uncommon and desirable as gasoline-surrogate biofuels. The biosynthetic pathway for NTE production was explored using metabolic labeling and GCMS. Two-carbon incorporation 13C acetate suggests that it is derived from a polyketide synthase (PKS) followed by decarboxylation. There are several known mechanisms for such decarboxylation, though none have been discovered in fungi. Towards identifying the PKS responsible for the production of NTE, the genome of N. mackinnonii E5202H (ATCC SD-6839) was sequenced and assembled. Of the 32 PKSs present in the genome, 17 are predicted to contain sufficient domains for the production of NTE. These results exemplify the capacity of endophytic fungi to produce novel natural products that may have many uses, such as biologically derived fuels and commodity chemicals.
Keywords
Endophyte, natural product, Volatile Organic Compound, polyene, medium-chain hydrocarbon, biofuel, polyketide synthase
About this resource
- Canonical resource URI:
http://purl.org/phylo/treebase/phylows/study/TB2:S15775
- Other versions:
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- Show BibTeX reference
@ARTICLE{TreeBASE2Ref23177,
author = {Jeffrey J Shaw and Daniel J Spakowicz and Rahul S Dalal and Jared H Davis and Nina A Lehr and Brian F Dunican and Esteban A Orellana and Alexandra Narv?ez-Trujillo and Scott A Strobel},
title = {Biosynthesis and genomic analysis of medium-chain hydrocarbon production by the endophytic fungal isolate Nigrograna mackinonnii (E5202H)},
year = {2014},
keywords = {Endophyte, natural product, Volatile Organic Compound, polyene, medium-chain hydrocarbon, biofuel, polyketide synthase},
doi = {},
url = {},
pmid = {},
journal = {Applied Microbiology and Biotechnology},
volume = {},
number = {},
pages = {},
abstract = {An endophytic fungus was isolated that produces a series of volatile natural products, including terpenes and odd chain polyenes. Phylogenetic analysis of the isolate using five loci suggests that it is closely related to Nigrograna mackinnonii CBS 674.75. The main component of the polyene series was purified and identified as (3E,5E,7E)-nona-1,3,5,7-tetraene (NTE), a novel natural product. Non-oxygenated hydrocarbons of this chain length are uncommon and desirable as gasoline-surrogate biofuels. The biosynthetic pathway for NTE production was explored using metabolic labeling and GCMS. Two-carbon incorporation 13C acetate suggests that it is derived from a polyketide synthase (PKS) followed by decarboxylation. There are several known mechanisms for such decarboxylation, though none have been discovered in fungi. Towards identifying the PKS responsible for the production of NTE, the genome of N. mackinnonii E5202H (ATCC SD-6839) was sequenced and assembled. Of the 32 PKSs present in the genome, 17 are predicted to contain sufficient domains for the production of NTE. These results exemplify the capacity of endophytic fungi to produce novel natural products that may have many uses, such as biologically derived fuels and commodity chemicals.}
}
- Show RIS reference
TY - JOUR
ID - 23177
AU - Shaw,Jeffrey J
AU - Spakowicz,Daniel J
AU - Dalal,Rahul S
AU - Davis,Jared H
AU - Lehr,Nina A
AU - Dunican,Brian F
AU - Orellana,Esteban A
AU - Narv?ez-Trujillo,Alexandra
AU - Strobel,Scott A
T1 - Biosynthesis and genomic analysis of medium-chain hydrocarbon production by the endophytic fungal isolate Nigrograna mackinonnii (E5202H)
PY - 2014
KW - Endophyte
KW - natural product
KW - Volatile Organic Compound
KW - polyene
KW - medium-chain hydrocarbon
KW - biofuel
KW - polyketide synthase
UR -
N2 - An endophytic fungus was isolated that produces a series of volatile natural products, including terpenes and odd chain polyenes. Phylogenetic analysis of the isolate using five loci suggests that it is closely related to Nigrograna mackinnonii CBS 674.75. The main component of the polyene series was purified and identified as (3E,5E,7E)-nona-1,3,5,7-tetraene (NTE), a novel natural product. Non-oxygenated hydrocarbons of this chain length are uncommon and desirable as gasoline-surrogate biofuels. The biosynthetic pathway for NTE production was explored using metabolic labeling and GCMS. Two-carbon incorporation 13C acetate suggests that it is derived from a polyketide synthase (PKS) followed by decarboxylation. There are several known mechanisms for such decarboxylation, though none have been discovered in fungi. Towards identifying the PKS responsible for the production of NTE, the genome of N. mackinnonii E5202H (ATCC SD-6839) was sequenced and assembled. Of the 32 PKSs present in the genome, 17 are predicted to contain sufficient domains for the production of NTE. These results exemplify the capacity of endophytic fungi to produce novel natural products that may have many uses, such as biologically derived fuels and commodity chemicals.
L3 -
JF - Applied Microbiology and Biotechnology
VL -
IS -
ER -
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