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Citation for Study 15937

Citation title: "The genome and occlusion bodies of marine Penaeus monodon nudivirus (PmNV, also known as MBV and PemoNPV) suggest that it should be assigned to a new nudivirus genus that is distinct from the terrestrial nudiviruses.".

Study name: "The genome and occlusion bodies of marine Penaeus monodon nudivirus (PmNV, also known as MBV and PemoNPV) suggest that it should be assigned to a new nudivirus genus that is distinct from the terrestrial nudiviruses.".

This study is part of submission 15937 (Status: Published).

Citation

Yang Y., Lee D., Wang Y., Hu J., Li W., Leu J., Chang G., Ke H., Kang S., Lin S., Kou G., & Lo C. 2014. The genome and occlusion bodies of marine Penaeus monodon nudivirus (PmNV, also known as MBV and PemoNPV) suggest that it should be assigned to a new nudivirus genus that is distinct from the terrestrial nudiviruses. BMC Genomics, 15(628).

Authors

• Yang Y. (submitter)
• Lee D.
• Wang Y.
• Hu J.
• Li W.
• Leu J.
• Chang G.
• Ke H.
• Kang S.
• Lin S.
• Kou G.
• Lo C.

Abstract

Background: Penaeus monodon nudivirus (PmNV) is the causative agent of spherical baculovirosis in shrimp (Penaeus monodon). This disease causes significant mortalities at the larval stage and early postlarval (PL) stage and may suppress growth and reduce survival and production in aquaculture. The nomenclature and classification status of PmNV has been changed several times due to morphological observation and phylogenetic analysis of its partial genome sequence. In this study, we therefore completed the genome sequence and constructed phylogenetic trees to clarify PmNV?s taxonomic position. To better understand the characteristics of the occlusion bodies formed by this marine occluded virus, we also compared the chemical properties of the polyhedrin produced by PmNV and the baculovirus AcMNPV (Autographa californica nucleopolyhedrovirus). Results: We used next generation sequencing and traditional PCR methods to obtain the complete PmNV genome sequence of 119,638 bp encoding 115 putative ORFs. Phylogenetic tree analysis showed that several PmNV genes and sequences clustered with the non-occluded nudiviruses and not with the baculoviruses. We also investigated the characteristics of PmNV polyhedrin, which is a functionally important protein and the major component of the viral OBs (occlusion bodies). We found that both recombinant PmNV polyhedrin and wild-type PmNV OBs were sensitive to acid conditions, but unlike the baculoviral OBs, they were not susceptible to alkali treatment. Conclusions: From the viral genome features and phylogenetic analysis we conclude that PmNV is not a baculovirus, and that it should be assigned to the proposed Nudiviridae family with the other nudiviruses, but into a distinct new genus (Gammanudivirus).

Keywords

PmNV, genome, baculovirus, nudivirus, OBs, polyhedrin

External links

• DOI: 10.1186/1471-2164-15-628
• Other URL: http://www.biomedcentral.com/1471-2164/15/628

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S15937
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref23304,
  	 author = {Yi-Ting  Yang and Der-Yen  Lee and Yongjie  Wang and Jer-Ming   Hu and Wen-Hsiung  Li and Jiann-Horng  Leu and Geen-Dong  Chang and Huei-Mien  Ke and Shin-Ting  Kang and Shih-Shun  Lin and Guang-Hsiung  Kou and Chu-Fang  Lo},
  	 title = {The genome and occlusion bodies of marine Penaeus monodon nudivirus (PmNV, also known as MBV and PemoNPV) suggest that it should be assigned to a new nudivirus genus that is distinct from the terrestrial nudiviruses.},
  	 year = {2014},
  	 keywords = {PmNV, genome, baculovirus, nudivirus, OBs, polyhedrin},
  	 doi = {10.1186/1471-2164-15-628},
  	 url = {http://www.biomedcentral.com/1471-2164/15/628},
  	 pmid = {},
  	 journal = {BMC Genomics},
  	 volume = {15},
  	 number = {628},
  	 pages = {},
  	 abstract = {Background: 
  Penaeus monodon nudivirus (PmNV) is the causative agent of spherical baculovirosis in shrimp (Penaeus monodon). This disease causes significant mortalities at the larval stage and early postlarval (PL) stage and may suppress growth and reduce survival and production in aquaculture. The nomenclature and classification status of PmNV has been changed several times due to morphological observation and phylogenetic analysis of its partial genome sequence. In this study, we therefore completed the genome sequence and constructed phylogenetic trees to clarify PmNV?s taxonomic position. To better understand the characteristics of the occlusion bodies formed by this marine occluded virus, we also compared the chemical properties of the polyhedrin produced by PmNV and the baculovirus AcMNPV (Autographa californica nucleopolyhedrovirus).
  
  Results: 
  We used next generation sequencing and traditional PCR methods to obtain the complete PmNV genome sequence of 119,638 bp encoding 115 putative ORFs. Phylogenetic tree analysis showed that several PmNV genes and sequences clustered with the non-occluded nudiviruses and not with the baculoviruses. We also investigated the characteristics of PmNV polyhedrin, which is a functionally important protein and the major component of the viral OBs (occlusion bodies). We found that both recombinant PmNV polyhedrin and wild-type PmNV OBs were sensitive to acid conditions, but unlike the baculoviral OBs, they were not susceptible to alkali treatment.
  
  Conclusions:
  From the viral genome features and phylogenetic analysis we conclude that PmNV is not a baculovirus, and that it should be assigned to the proposed Nudiviridae family with the other nudiviruses, but into a distinct new genus (Gammanudivirus).
  }
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 23304
  AU  - Yang,Yi-Ting 
  AU  - Lee,Der-Yen 
  AU  - Wang,Yongjie 
  AU  - Hu,Jer-Ming  
  AU  - Li,Wen-Hsiung 
  AU  - Leu,Jiann-Horng 
  AU  - Chang,Geen-Dong 
  AU  - Ke,Huei-Mien 
  AU  - Kang,Shin-Ting 
  AU  - Lin,Shih-Shun 
  AU  - Kou,Guang-Hsiung 
  AU  - Lo,Chu-Fang 
  T1  - The genome and occlusion bodies of marine Penaeus monodon nudivirus (PmNV, also known as MBV and PemoNPV) suggest that it should be assigned to a new nudivirus genus that is distinct from the terrestrial nudiviruses.
  PY  - 2014
  KW  - PmNV
  KW  - genome
  KW  - baculovirus
  KW  - nudivirus
  KW  - OBs
  KW  - polyhedrin
  UR  - http://www.biomedcentral.com/1471-2164/15/628
  N2  - Background: 
  Penaeus monodon nudivirus (PmNV) is the causative agent of spherical baculovirosis in shrimp (Penaeus monodon). This disease causes significant mortalities at the larval stage and early postlarval (PL) stage and may suppress growth and reduce survival and production in aquaculture. The nomenclature and classification status of PmNV has been changed several times due to morphological observation and phylogenetic analysis of its partial genome sequence. In this study, we therefore completed the genome sequence and constructed phylogenetic trees to clarify PmNV?s taxonomic position. To better understand the characteristics of the occlusion bodies formed by this marine occluded virus, we also compared the chemical properties of the polyhedrin produced by PmNV and the baculovirus AcMNPV (Autographa californica nucleopolyhedrovirus).
  
  Results: 
  We used next generation sequencing and traditional PCR methods to obtain the complete PmNV genome sequence of 119,638 bp encoding 115 putative ORFs. Phylogenetic tree analysis showed that several PmNV genes and sequences clustered with the non-occluded nudiviruses and not with the baculoviruses. We also investigated the characteristics of PmNV polyhedrin, which is a functionally important protein and the major component of the viral OBs (occlusion bodies). We found that both recombinant PmNV polyhedrin and wild-type PmNV OBs were sensitive to acid conditions, but unlike the baculoviral OBs, they were not susceptible to alkali treatment.
  
  Conclusions:
  From the viral genome features and phylogenetic analysis we conclude that PmNV is not a baculovirus, and that it should be assigned to the proposed Nudiviridae family with the other nudiviruses, but into a distinct new genus (Gammanudivirus).
  
  L3  - 10.1186/1471-2164-15-628
  JF  - BMC Genomics
  VL  - 15
  IS  - 628
  ER  -