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Citation for Study 16436

Citation title: "Characterization of Two Species of Trypanosomatidae from the Honey Bee Apis mellifera: Crithidia mellificae Langridge and McGhee, 1967 and Lotmaria passim n. gen., n. sp.".

Study name: "Characterization of Two Species of Trypanosomatidae from the Honey Bee Apis mellifera: Crithidia mellificae Langridge and McGhee, 1967 and Lotmaria passim n. gen., n. sp.".

This study is part of submission 16436 (Status: Published).

Citation

Schwarz R.S., Bauchan G.R., Murphy C.A., Ravoet J., De graaf D.C., & Evans J.D. 2015. Characterization of Two Species of Trypanosomatidae from the Honey Bee Apis mellifera: Crithidia mellificae Langridge and McGhee, 1967 and Lotmaria passim n. gen., n. sp. Journal of Eukaryotic Microbiology, .

Authors

• Schwarz R.S. (submitter)
• Bauchan G.R.
• Murphy C.A.
• Ravoet J.
• De graaf D.C.
• Evans J.D.

Abstract

Trypanosomatids are increasingly recognized as prevalent in European honey bees (Apis mellifera) and by default are attributed to one recognized species, Crithidia mellificae Langridge and McGhee, 1967. We provide reference genetic and ultrastructural data for type isolates of C. mellificae (ATCC 30254 and 30862) in comparison with two recent isolates from A. mellifera (BRL and SF). Phylogenetics unambiguously identify strains BRL/SF as a novel taxonomic unit distinct from C. mellificae strains 30254/30862 and assign all four strains as lineages of a novel clade within the subfamily Leishmaniinae. In vivo analyses show strains BRL/SF preferably colonize the hindgut, lining the lumen as adherent spheroids in a manner identical to previous descriptions from C. mellificae. Microscopy images show motile forms of C. mellificae are distinct from strains BRL/SF. We propose the binomial Lotmaria passim n. gen., n. sp. for this previously undescribed taxon. Analyses of new and previously accessioned genetic data show C. mellificae is still extant in bee populations, however L. passim n. gen., n. sp. is currently the predominant trypanosomatid in A. mellifera globally. Our findings require that previous reports of C. mellificae be reconsidered and that subsequent trypanosomatid species designations from Hymenoptera provide genetic support.

Keywords

Apidae; flagellate; Kinetoplastea; Leptomonas; protists; systematics; taxonomy; ultrastructure

External links

• DOI: 10.1111/jeu.12209-4607

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S16436
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref23709,
  	 author = {Ryan S Schwarz and Gary R. Bauchan and Charles A. Murphy and Jorgen  Ravoet and Dirk C. de Graaf and Jay D. Evans},
  	 title = {Characterization of Two Species of Trypanosomatidae from the Honey Bee Apis mellifera: Crithidia mellificae Langridge and McGhee, 1967 and Lotmaria passim n. gen., n. sp.},
  	 year = {2015},
  	 keywords = {Apidae; flagellate; Kinetoplastea; Leptomonas; protists; systematics; taxonomy; ultrastructure},
  	 doi = {10.1111/jeu.12209-4607},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Journal of Eukaryotic Microbiology},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Trypanosomatids are increasingly recognized as prevalent in European honey bees (Apis mellifera) and by default are attributed to one recognized species, Crithidia mellificae Langridge and McGhee, 1967.  We provide reference genetic and ultrastructural data for type isolates of C. mellificae (ATCC 30254 and 30862) in comparison with two recent isolates from A. mellifera (BRL and SF).  Phylogenetics unambiguously identify strains BRL/SF as a novel taxonomic unit distinct from C. mellificae strains 30254/30862 and assign all four strains as lineages of a novel clade within the subfamily Leishmaniinae.  In vivo analyses show strains BRL/SF preferably colonize the hindgut, lining the lumen as adherent spheroids in a manner identical to previous descriptions from C. mellificae.  Microscopy images show motile forms of C. mellificae are distinct from strains BRL/SF.  We propose the binomial Lotmaria passim n. gen., n. sp. for this previously undescribed taxon.  Analyses of new and previously accessioned genetic data show C. mellificae is still extant in bee populations, however L. passim n. gen., n. sp. is currently the predominant trypanosomatid in A. mellifera globally.  Our findings require that previous reports of C. mellificae be reconsidered and that subsequent trypanosomatid species designations from Hymenoptera provide genetic support.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 23709
  AU  - Schwarz,Ryan S
  AU  - Bauchan,Gary R.
  AU  - Murphy,Charles A.
  AU  - Ravoet,Jorgen 
  AU  - de Graaf,Dirk C.
  AU  - Evans,Jay D.
  T1  - Characterization of Two Species of Trypanosomatidae from the Honey Bee Apis mellifera: Crithidia mellificae Langridge and McGhee, 1967 and Lotmaria passim n. gen., n. sp.
  PY  - 2015
  KW  - Apidae; flagellate; Kinetoplastea; Leptomonas; protists; systematics; taxonomy; ultrastructure
  UR  - http://dx.doi.org/10.1111/jeu.12209-4607
  N2  - Trypanosomatids are increasingly recognized as prevalent in European honey bees (Apis mellifera) and by default are attributed to one recognized species, Crithidia mellificae Langridge and McGhee, 1967.  We provide reference genetic and ultrastructural data for type isolates of C. mellificae (ATCC 30254 and 30862) in comparison with two recent isolates from A. mellifera (BRL and SF).  Phylogenetics unambiguously identify strains BRL/SF as a novel taxonomic unit distinct from C. mellificae strains 30254/30862 and assign all four strains as lineages of a novel clade within the subfamily Leishmaniinae.  In vivo analyses show strains BRL/SF preferably colonize the hindgut, lining the lumen as adherent spheroids in a manner identical to previous descriptions from C. mellificae.  Microscopy images show motile forms of C. mellificae are distinct from strains BRL/SF.  We propose the binomial Lotmaria passim n. gen., n. sp. for this previously undescribed taxon.  Analyses of new and previously accessioned genetic data show C. mellificae is still extant in bee populations, however L. passim n. gen., n. sp. is currently the predominant trypanosomatid in A. mellifera globally.  Our findings require that previous reports of C. mellificae be reconsidered and that subsequent trypanosomatid species designations from Hymenoptera provide genetic support.
  L3  - 10.1111/jeu.12209-4607
  JF  - Journal of Eukaryotic Microbiology
  VL  - 
  IS  - 
  ER  -