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Citation for Study 16597

Citation title: "An 18S and 28S-based clock calibration for marine Proseriata (Platyhelminthes)".

Study name: "An 18S and 28S-based clock calibration for marine Proseriata (Platyhelminthes)".

This study is part of submission 16597 (Status: Published).

Citation

Scarpa F., Cossu P., Sanna D., Lai T., Norenburg J.L., Curini-galletti M., & Casu M. 2015. An 18S and 28S-based clock calibration for marine Proseriata (Platyhelminthes). Journal of Experimental Marine Biology and Ecology, 463: 22-31.

Authors

• Scarpa F.
• Cossu P.
• Sanna D.
• Lai T.
• Norenburg J.L.
• Curini-galletti M.
• Casu M.

Abstract

Geminate species are a powerful tool for calibrating the molecular clock in marine organisms, and their adoption is mandatory for soft-bodied taxa, which lack fossil records. The first attempt to calibrate the molecular clock in taxa belonging to meiofaunal microturbellaria (Platyhelminthes: Proseriata) based on geminate species is presented here. We used two species pairs from both sides of the Isthmus of Panama: Minona gemella (Caribbean) and Minona cf gemella (Pacific); Parotoplana sp. nov. 1 (Caribbean) and Parotoplana sp. nov. 2 (Pacific). The mutation rates per million years were estimated for both geminate species pairs on two ribosomal regions, the complete nuclear small subunit rDNA (18S) gene and the partial nuclear large subunit rDNA (28S) gene fragment (spanning variable domains D1-D6). Similar values of mutation rates per million years were found in both species pairs, ranging 0.12-0.16% for 18S and 0.49-0.52% for 28S. The values obtained were used as calibration points at minimum age, in order to estimate the divergence times within the phylogenetic tree of the whole dataset, and tested on three cases of trans-American (not-geminate) species from Pacific Panama and S-E Brazil, belonging to the genera Kata, Archimonocelis and Duplominona. They consistently showed higher divergence times (ranging 9.4-17.9 Myr) than geminate, trans-isthmian pairs. These results suggest potential usefulness of our molecular clock calibration, for future research on phylogeography and evolution of Proseriata.

Keywords

Meiofauna; Isthmus of Panama; mutation rates; relaxed molecular clock; divergence time; calibration point.

External links

• DOI: 10.1016/j.jembe.2014.10.020

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S16597
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref23824,
  	 author = {Fabio  Scarpa and Piero  Cossu and Daria  Sanna and Tiziana  Lai and Jon L. Norenburg and Marco  Curini-Galletti and Marco  Casu},
  	 title = {An 18S and 28S-based clock calibration for marine Proseriata (Platyhelminthes)},
  	 year = {2015},
  	 keywords = {Meiofauna; Isthmus of Panama; mutation rates; relaxed molecular clock; divergence time; calibration point.},
  	 doi = {10.1016/j.jembe.2014.10.020},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Journal of Experimental Marine Biology and Ecology},
  	 volume = {463},
  	 number = {},
  	 pages = {22--31},
  	 abstract = {Geminate species are a powerful tool for calibrating the molecular clock in marine organisms, and their adoption is mandatory for soft-bodied taxa, which lack fossil records. The first attempt to calibrate the molecular clock in taxa belonging to meiofaunal microturbellaria (Platyhelminthes: Proseriata) based on geminate species is presented here. We used two species pairs from both sides of the Isthmus of Panama: Minona gemella (Caribbean) and Minona cf gemella (Pacific); Parotoplana sp. nov. 1 (Caribbean) and Parotoplana sp. nov. 2 (Pacific). The mutation rates per million years were estimated for both geminate species pairs on two ribosomal regions, the complete nuclear small subunit rDNA (18S) gene and the partial nuclear large subunit rDNA (28S) gene fragment (spanning variable domains D1-D6). Similar values of mutation rates per million years were found in both species pairs, ranging 0.12-0.16% for 18S and 0.49-0.52% for 28S. The values obtained were used as calibration points at minimum age, in order to estimate the divergence times within the phylogenetic tree of the whole dataset, and tested on three cases of trans-American (not-geminate) species from Pacific Panama and S-E Brazil, belonging to the genera Kata, Archimonocelis and Duplominona. They consistently showed higher divergence times (ranging 9.4-17.9 Myr) than geminate, trans-isthmian pairs. These results suggest potential usefulness of our molecular clock calibration, for future research on phylogeography and evolution of Proseriata. }
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 23824
  AU  - Scarpa,Fabio 
  AU  - Cossu,Piero 
  AU  - Sanna,Daria 
  AU  - Lai,Tiziana 
  AU  - Norenburg,Jon L.
  AU  - Curini-Galletti,Marco 
  AU  - Casu,Marco 
  T1  - An 18S and 28S-based clock calibration for marine Proseriata (Platyhelminthes)
  PY  - 2015
  KW  - Meiofauna; Isthmus of Panama; mutation rates; relaxed molecular clock; divergence time; calibration point.
  UR  - http://dx.doi.org/10.1016/j.jembe.2014.10.020
  N2  - Geminate species are a powerful tool for calibrating the molecular clock in marine organisms, and their adoption is mandatory for soft-bodied taxa, which lack fossil records. The first attempt to calibrate the molecular clock in taxa belonging to meiofaunal microturbellaria (Platyhelminthes: Proseriata) based on geminate species is presented here. We used two species pairs from both sides of the Isthmus of Panama: Minona gemella (Caribbean) and Minona cf gemella (Pacific); Parotoplana sp. nov. 1 (Caribbean) and Parotoplana sp. nov. 2 (Pacific). The mutation rates per million years were estimated for both geminate species pairs on two ribosomal regions, the complete nuclear small subunit rDNA (18S) gene and the partial nuclear large subunit rDNA (28S) gene fragment (spanning variable domains D1-D6). Similar values of mutation rates per million years were found in both species pairs, ranging 0.12-0.16% for 18S and 0.49-0.52% for 28S. The values obtained were used as calibration points at minimum age, in order to estimate the divergence times within the phylogenetic tree of the whole dataset, and tested on three cases of trans-American (not-geminate) species from Pacific Panama and S-E Brazil, belonging to the genera Kata, Archimonocelis and Duplominona. They consistently showed higher divergence times (ranging 9.4-17.9 Myr) than geminate, trans-isthmian pairs. These results suggest potential usefulness of our molecular clock calibration, for future research on phylogeography and evolution of Proseriata. 
  L3  - 10.1016/j.jembe.2014.10.020
  JF  - Journal of Experimental Marine Biology and Ecology
  VL  - 463
  IS  - 
  SP  - 22
  EP  - 31
  ER  -