CiteULike

Delicious

Connotea

Citation for Study 16794

Citation title: "Death of endemic Virgilia oroboides trees in a South African national botanical garden caused by Diaporthe virgiliae sp. nov. ".

Study name: "Death of endemic Virgilia oroboides trees in a South African national botanical garden caused by Diaporthe virgiliae sp. nov. ".

This study is part of submission 16794 (Status: Published).

Citation

Machingambi N.M., Dreyer L., Oberlander K.C., Roux J., & Roets F. 2014. Death of endemic Virgilia oroboides trees in a South African national botanical garden caused by Diaporthe virgiliae sp. nov. Plant Pathology, .

Authors

• Machingambi N.M.
• Dreyer L.
• Oberlander K.C. (submitter) +27 82 394 2344
• Roux J.
• Roets F.

Abstract

Numerous dead and dying individuals of the Western Cape endemic tree Virgilia oroboides (Fabaceae) were recently observed within a South African national botanical garden. Root-rot fungi and bark beetle (Curculionidae; Scolytinae) symbiotic fungi from diseased trees were assessed for their respective roles in V. oroboides mortality. Disease progression in this V. oroboides population was also monitored over one year. Fungi were isolated from surface sterilised bark and root samples from diseased trees and were provisionally identified using data from the internal transcribed spacer regions (ITS1, ITS2), including the 5.8S gene (ITS). Pathogenicity of selected fungi towards V. oroboides was tested using artificial inoculation studies under field conditions. The pathogenicity of various bark beetle associated Geosmithia (Hypocreales: Hypocreomycetidae) spp. from V. oroboides, were similarly assessed. The only fungus consistently isolated from lesions on the roots and bark of declining V. oroboides, and never from healthy individuals, represented an un-described Diaporthe (Diaporthales, Diaporthaceae) sp. that was characterised using molecular - (using data from the ITS marker and part of the β-tubulin gene (TUB)), culture and morphological characters. It is an aggressive pathogen of V. oroboides, newly described here as Diaporthe virgiliae sp. nov. Trees of all ages are susceptible to this pathogen with subsequent bark beetle attack of mature trees only. All Geosmithia spp. were non-pathogenic towards V. oroboides. Diaporthe virgiliae caused a significant decline in the health of the monitored V. oroboides population over a period of only one year and should be considered as a significant threat to these trees.

Keywords

root pathogen, tree death, Phomopsis, Geosmithia, bark beetle

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S16794
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref23984,
  	 author = {Netsai M. Machingambi and Leanne L.  Dreyer and Kenneth Carl Oberlander and Jolanda  Roux and Francois  Roets},
  	 title = {Death of endemic Virgilia oroboides trees in a South African national botanical garden caused by Diaporthe virgiliae sp. nov.  },
  	 year = {2014},
  	 keywords = {root pathogen, tree death, Phomopsis, Geosmithia, bark beetle},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Plant Pathology},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Numerous dead and dying individuals of the Western Cape endemic tree Virgilia oroboides (Fabaceae) were recently observed within a South African national botanical garden. Root-rot fungi and bark beetle (Curculionidae; Scolytinae) symbiotic fungi from diseased trees were assessed for their respective roles in V. oroboides mortality. Disease progression in this V. oroboides population was also monitored over one year. Fungi were isolated from surface sterilised bark and root samples from diseased trees and were provisionally identified using data from the internal transcribed spacer regions (ITS1, ITS2), including the 5.8S gene (ITS). Pathogenicity of selected fungi towards V. oroboides was tested using artificial inoculation studies under field conditions. The pathogenicity of various bark beetle associated Geosmithia (Hypocreales: Hypocreomycetidae) spp. from V. oroboides, were similarly assessed. The only fungus consistently isolated from lesions on the roots and bark of declining V. oroboides, and never from healthy individuals, represented an un-described Diaporthe (Diaporthales, Diaporthaceae) sp. that was characterised using molecular - (using data from the ITS marker and part of the β-tubulin gene (TUB)), culture and morphological characters. It is an aggressive pathogen of V. oroboides, newly described here as Diaporthe virgiliae sp. nov. Trees of all ages are susceptible to this pathogen with subsequent bark beetle attack of mature trees only. All Geosmithia spp. were non-pathogenic towards V. oroboides. Diaporthe virgiliae caused a significant decline in the health of the monitored V. oroboides population over a period of only one year and should be considered as a significant threat to these trees. }
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 23984
  AU  - Machingambi,Netsai M.
  AU  - Dreyer,Leanne L. 
  AU  - Oberlander,Kenneth Carl
  AU  - Roux,Jolanda 
  AU  - Roets,Francois 
  T1  - Death of endemic Virgilia oroboides trees in a South African national botanical garden caused by Diaporthe virgiliae sp. nov.  
  PY  - 2014
  KW  - root pathogen
  KW  - tree death
  KW  - Phomopsis
  KW  - Geosmithia
  KW  - bark beetle
  UR  - http://dx.doi.org/
  N2  - Numerous dead and dying individuals of the Western Cape endemic tree Virgilia oroboides (Fabaceae) were recently observed within a South African national botanical garden. Root-rot fungi and bark beetle (Curculionidae; Scolytinae) symbiotic fungi from diseased trees were assessed for their respective roles in V. oroboides mortality. Disease progression in this V. oroboides population was also monitored over one year. Fungi were isolated from surface sterilised bark and root samples from diseased trees and were provisionally identified using data from the internal transcribed spacer regions (ITS1, ITS2), including the 5.8S gene (ITS). Pathogenicity of selected fungi towards V. oroboides was tested using artificial inoculation studies under field conditions. The pathogenicity of various bark beetle associated Geosmithia (Hypocreales: Hypocreomycetidae) spp. from V. oroboides, were similarly assessed. The only fungus consistently isolated from lesions on the roots and bark of declining V. oroboides, and never from healthy individuals, represented an un-described Diaporthe (Diaporthales, Diaporthaceae) sp. that was characterised using molecular - (using data from the ITS marker and part of the β-tubulin gene (TUB)), culture and morphological characters. It is an aggressive pathogen of V. oroboides, newly described here as Diaporthe virgiliae sp. nov. Trees of all ages are susceptible to this pathogen with subsequent bark beetle attack of mature trees only. All Geosmithia spp. were non-pathogenic towards V. oroboides. Diaporthe virgiliae caused a significant decline in the health of the monitored V. oroboides population over a period of only one year and should be considered as a significant threat to these trees. 
  L3  - 
  JF  - Plant Pathology
  VL  - 
  IS  - 
  ER  -