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Citation for Study 17564

Citation title: "Morphology and phylogenetic position of a rare four-celled green alga, Pascherina tetras (Volvocales, Chlorophyceae), based on cultured material".

Study name: "Morphology and phylogenetic position of a rare four-celled green alga, Pascherina tetras (Volvocales, Chlorophyceae), based on cultured material".

This study is part of submission 17564 (Status: Published).

Citation

Sugasawa M., Matsuzaki R., Arakaki Y., & Nozaki H. 2015. Morphology and phylogenetic position of a rare four-celled green alga, Pascherina tetras (Volvocales, Chlorophyceae), based on cultured material. Phycologia, 54(4): 342-348.

Authors

Sugasawa M.
Matsuzaki R. (submitter) +81-29-850-2204
Arakaki Y.
Nozaki H. +81-3-5841-4048

Abstract

Since the original description in 1928, four-celled colonies of the spondylomoracean genus Pascherina have not been studied in culture due to a lack of reliable culture strains of this genus for reexamination and molecular phylogenetic analyses. Here, we established new culture strains of Pascherina tetras, the only species of the genus, from Japanese samples and conducted detailed light and transmission electron microscopic observations and a molecular phylogenic analysis based on the strains. The light microscopic features of the Japanese material were consistent with those of the original material collected in Ukraine, except for the stigma of the chloroplast; the stigma of the Japanese strains was ring-shaped based on a surface view. The ring-shaped stigma was confirmed by transmission electron microscopy. Our molecular phylogenetic analysis revealed that Pascherina was robustly separated from the other three colonial volvocalean lineages, Stephanosphaera (Haematococcaceae), colonial volvocine algae, and Pyrobotrys (Spondylomoraceae). The present morphological and molecular analyses represent the first study to demonstrate the polyphyly of Spondylomoraceae.

Keywords

culture strain, molecular phylogeny, Spondylomoraceae, ultrastructure

External links

DOI: 10.2216/15-27.1
Other URL: http://www.phycologia.org/doi/abs/10.2216/15-27.1

About this resource

Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S17564
Other versions: Nexus NeXML

Show BibTeX reference

		@ARTICLE{TreeBASE2Ref24555,
	 author = {Mizuho  Sugasawa and Ryo  Matsuzaki and Yoko  Arakaki and Hisayoshi  Nozaki},
	 title = {Morphology and phylogenetic position of a rare four-celled green alga, Pascherina tetras (Volvocales, Chlorophyceae), based on cultured material},
	 year = {2015},
	 keywords = {culture strain, molecular phylogeny, Spondylomoraceae, ultrastructure},
	 doi = {10.2216/15-27.1},
	 url = {http://www.phycologia.org/doi/abs/10.2216/15-27.1},
	 pmid = {},
	 journal = {Phycologia},
	 volume = {54},
	 number = {4},
	 pages = {342--348},
	 abstract = {Since the original description in 1928, four-celled colonies of the spondylomoracean genus Pascherina have not been studied in culture due to a lack of reliable culture strains of this genus for reexamination and molecular phylogenetic analyses. Here, we established new culture strains of Pascherina tetras, the only species of the genus, from Japanese samples and conducted detailed light and transmission electron microscopic observations and a molecular phylogenic analysis based on the strains. The light microscopic features of the Japanese material were consistent with those of the original material collected in Ukraine, except for the stigma of the chloroplast; the stigma of the Japanese strains was ring-shaped based on a surface view. The ring-shaped stigma was confirmed by transmission electron microscopy. Our molecular phylogenetic analysis revealed that Pascherina was robustly separated from the other three colonial volvocalean lineages, Stephanosphaera (Haematococcaceae), colonial volvocine algae, and Pyrobotrys (Spondylomoraceae). The present morphological and molecular analyses represent the first study to demonstrate the polyphyly of Spondylomoraceae.}
}

Show RIS reference

		TY  - JOUR
ID  - 24555
AU  - Sugasawa,Mizuho 
AU  - Matsuzaki,Ryo 
AU  - Arakaki,Yoko 
AU  - Nozaki,Hisayoshi 
T1  - Morphology and phylogenetic position of a rare four-celled green alga, Pascherina tetras (Volvocales, Chlorophyceae), based on cultured material
PY  - 2015
KW  - culture strain
KW  - molecular phylogeny
KW  - Spondylomoraceae
KW  - ultrastructure
UR  - http://www.phycologia.org/doi/abs/10.2216/15-27.1
N2  - Since the original description in 1928, four-celled colonies of the spondylomoracean genus Pascherina have not been studied in culture due to a lack of reliable culture strains of this genus for reexamination and molecular phylogenetic analyses. Here, we established new culture strains of Pascherina tetras, the only species of the genus, from Japanese samples and conducted detailed light and transmission electron microscopic observations and a molecular phylogenic analysis based on the strains. The light microscopic features of the Japanese material were consistent with those of the original material collected in Ukraine, except for the stigma of the chloroplast; the stigma of the Japanese strains was ring-shaped based on a surface view. The ring-shaped stigma was confirmed by transmission electron microscopy. Our molecular phylogenetic analysis revealed that Pascherina was robustly separated from the other three colonial volvocalean lineages, Stephanosphaera (Haematococcaceae), colonial volvocine algae, and Pyrobotrys (Spondylomoraceae). The present morphological and molecular analyses represent the first study to demonstrate the polyphyly of Spondylomoraceae.
L3  - 10.2216/15-27.1
JF  - Phycologia
VL  - 54
IS  - 4
SP  - 342
EP  - 348
ER  -