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Citation for Study 17972

Citation title: "Genome-wide identification and transcriptional analysis of folate metabolism-related genes in maize kernels ".

Study name: "Genome-wide identification and transcriptional analysis of folate metabolism-related genes in maize kernels ".

This study is part of submission 17972 (Status: Published).

Citation

Lian T., Guo W., Chen M., Li J., Liang Q., Liu F., Meng H., Xu B., Chen J., Zhang C., & Jiang L. 2015. Genome-wide identification and transcriptional analysis of folate metabolism-related genes in maize kernels. BMC Plant Biology, .

Authors

• Lian T.
• Guo W.
• Chen M.
• Li J.
• Liang Q.
• Liu F.
• Meng H.
• Xu B.
• Chen J.
• Zhang C.
• Jiang L.

Abstract

Abstract Background: Maize is a major staple food crop globally and contains various concentrations of vitamins. Folates are essential water-soluble B-vitamins that play an important role as one-carbon (C1) donors and acceptors in organisms. To gain an understanding of folate metabolism in maize, we performed an intensive in silico analysis to screen for genes involved in folate metabolism using publicly available databases, followed by examination of the transcript expression patterns and profiling of the folate derivatives in the kernels of two maize inbred lines. Results: A total of 36 candidate genes corresponding to 16 folate metabolism-related enzymes were identified. The maize genome contains all the enzymes required for folate and C1 metabolism, characterized by highly conserved functional domains across all the other species investigated. Phylogenetic analysis revealed that these enzymes in maize are conserved throughout evolution and have a high level of similarity with those in sorghum and millet. The LC-MS analyses of two maize inbred lines demonstrated that 5-methyltetrahydrofolate was the major form of folate derivative in young seeds, while 5-formyltetrahydrofolate in mature seeds. Most of the genes involved in folate and C1 metabolism exhibited similar transcriptional expression patterns between these two maize lines, with the highest transcript abundance detected on day after pollination (DAP) 6 and the decreased transcript abundance on DAP 12 and 18. Compared with the seeds on DAP 30, 5-methyltetrahydrofolate was decreased and 5-formyltetrahydrofolate was increased sharply in the mature dry seeds. Conclusions: The enzymes involved in folate and C1 metabolism are conserved between maize and other plant species. Folate and C1 metabolism is active in young developing maize seeds at transcriptional levels.

Keywords

Maize, folate metabolism, C1 metabolism, expression pattern, folate profiling

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S17972
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref24866,
  	 author = {Tong  Lian and Wenzhu  Guo and Maoran  Chen and Jinglai  Li and Qiuju  Liang and Fang  Liu and Hongyan  Meng and Bosi  Xu and Jinfeng  Chen and Chunyi  Zhang and Ling  Jiang},
  	 title = {Genome-wide identification and transcriptional analysis of folate metabolism-related genes in maize kernels },
  	 year = {2015},
  	 keywords = {Maize, folate metabolism, C1 metabolism, expression pattern, folate profiling},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {BMC Plant Biology},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Abstract
  Background: Maize is a major staple food crop globally and contains various concentrations of vitamins. Folates are essential water-soluble B-vitamins that play an important role as one-carbon (C1) donors and acceptors in organisms. To gain an understanding of folate metabolism in maize, we performed an intensive in silico analysis to screen for genes involved in folate metabolism using publicly available databases, followed by examination of the transcript expression patterns and profiling of the folate derivatives in the kernels of two maize inbred lines.
  Results: A total of 36 candidate genes corresponding to 16 folate metabolism-related enzymes were identified. The maize genome contains all the enzymes required for folate and C1 metabolism, characterized by highly conserved functional domains across all the other species investigated. Phylogenetic analysis revealed that these enzymes in maize are conserved throughout evolution and have a high level of similarity with those in sorghum and millet. The LC-MS analyses of two maize inbred lines demonstrated that 5-methyltetrahydrofolate was the major form of folate derivative in young seeds, while 5-formyltetrahydrofolate in mature seeds. Most of the genes involved in folate and C1 metabolism exhibited similar transcriptional expression patterns between these two maize lines, with the highest transcript abundance detected on day after pollination (DAP) 6 and the decreased transcript abundance on DAP 12 and 18. Compared with the seeds on DAP 30, 5-methyltetrahydrofolate was decreased and 5-formyltetrahydrofolate was increased sharply in the mature dry seeds. 
  Conclusions: The enzymes involved in folate and C1 metabolism are conserved between maize and other plant species. Folate and C1 metabolism is active in young developing maize seeds at transcriptional levels.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 24866
  AU  - Lian,Tong 
  AU  - Guo,Wenzhu 
  AU  - Chen,Maoran 
  AU  - Li,Jinglai 
  AU  - Liang,Qiuju 
  AU  - Liu,Fang 
  AU  - Meng,Hongyan 
  AU  - Xu,Bosi 
  AU  - Chen,Jinfeng 
  AU  - Zhang,Chunyi 
  AU  - Jiang,Ling 
  T1  - Genome-wide identification and transcriptional analysis of folate metabolism-related genes in maize kernels 
  PY  - 2015
  KW  - Maize
  KW  - folate metabolism
  KW  - C1 metabolism
  KW  - expression pattern
  KW  - folate profiling
  UR  - http://dx.doi.org/
  N2  - Abstract
  Background: Maize is a major staple food crop globally and contains various concentrations of vitamins. Folates are essential water-soluble B-vitamins that play an important role as one-carbon (C1) donors and acceptors in organisms. To gain an understanding of folate metabolism in maize, we performed an intensive in silico analysis to screen for genes involved in folate metabolism using publicly available databases, followed by examination of the transcript expression patterns and profiling of the folate derivatives in the kernels of two maize inbred lines.
  Results: A total of 36 candidate genes corresponding to 16 folate metabolism-related enzymes were identified. The maize genome contains all the enzymes required for folate and C1 metabolism, characterized by highly conserved functional domains across all the other species investigated. Phylogenetic analysis revealed that these enzymes in maize are conserved throughout evolution and have a high level of similarity with those in sorghum and millet. The LC-MS analyses of two maize inbred lines demonstrated that 5-methyltetrahydrofolate was the major form of folate derivative in young seeds, while 5-formyltetrahydrofolate in mature seeds. Most of the genes involved in folate and C1 metabolism exhibited similar transcriptional expression patterns between these two maize lines, with the highest transcript abundance detected on day after pollination (DAP) 6 and the decreased transcript abundance on DAP 12 and 18. Compared with the seeds on DAP 30, 5-methyltetrahydrofolate was decreased and 5-formyltetrahydrofolate was increased sharply in the mature dry seeds. 
  Conclusions: The enzymes involved in folate and C1 metabolism are conserved between maize and other plant species. Folate and C1 metabolism is active in young developing maize seeds at transcriptional levels.
  L3  - 
  JF  - BMC Plant Biology
  VL  - 
  IS  - 
  ER  -