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Citation for Study 18308

Citation title: "Molecular phylogeny of the tropical lichen family Pyrenulaceae: contribution from dried herbarium specimens and FTA card samples".

Study name: "Molecular phylogeny of the tropical lichen family Pyrenulaceae: contribution from dried herbarium specimens and FTA card samples".

This study is part of submission 18308 (Status: Published).

Citation

Gueidan C., Aptroot A., Caceres M.E., & Binh N.Q. 2016. Molecular phylogeny of the tropical lichen family Pyrenulaceae: contribution from dried herbarium specimens and FTA card samples. Mycological Progress, 15(7).

Authors

• Gueidan C.
• Aptroot A.
• Caceres M.E.
• Binh N.Q.

Abstract

The Pyrenulaceae is one of the most common lichen families in tropical rainforests. These mostly lichenised ascomycete fungi are relatively well known, as identification tools are available for many members of the family, including the most species rich genus Pyrenula. However, despite the past boom in DNA sequence generation for most living organisms, molecular data is still mainly lacking for these crustose corticolous lichens, mainly because genomic DNA does not seem to preserve well after specimen desiccation. Because of lengthy custom procedures, newly collected specimens are therefore often too old for molecular work when they reach the laboratory. Here, a new method of DNA extraction and storage using FTA card samples was investigated. New DNA sequences were generated using both DNA extractions from freshly collected material (116 sequences) and FTA card samples (115 sequences) for 100 taxa within Pyrenulaceae. Inferences using three ribosomal genes (nuLSU, mtSSU and ITS) highlighted delimitation problems in few taxa, including the pantropical species P. mamillana and P. quassiicola. Issues related to generic delimitations were also confirmed, with Anthracothecium, Pyrgillus and Lithothelium nested within the species rich genus Pyrenula. Although further taxon and gene sampling will be required to fully revise species and generic concepts within this family, our data allowed us to describe two new species from Brazil (Lithothelium immersum and Pyrenula minutispora) and one from North America (Pyrenula reebiae).

Keywords

Anthracothecium, DNA extraction, Pyrenula, multigene phylogeny, Whatman FTA card

External links

• DOI: 10.1007/s11557-015-1154-8

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S18308
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref25135,
  	 author = {Cecile  Gueidan and Andr?  Aptroot and Marcela E.S. Caceres and Nguyen Quoc Binh},
  	 title = {Molecular phylogeny of the tropical lichen family Pyrenulaceae: contribution from dried herbarium specimens and FTA card samples},
  	 year = {2016},
  	 keywords = {Anthracothecium, DNA extraction, Pyrenula, multigene phylogeny, Whatman FTA card},
  	 doi = {10.1007/s11557-015-1154-8},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Mycological Progress},
  	 volume = {15},
  	 number = {7},
  	 pages = {},
  	 abstract = {The Pyrenulaceae is one of the most common lichen families in tropical rainforests. These mostly lichenised ascomycete fungi are relatively well known, as identification tools are available for many members of the family, including the most species rich genus Pyrenula. However, despite the past boom in DNA sequence generation for most living organisms, molecular data is still mainly lacking for these crustose corticolous lichens, mainly because genomic DNA does not seem to preserve well after specimen desiccation. Because of lengthy custom procedures, newly collected specimens are therefore often too old for molecular work when they reach the laboratory. Here, a new method of DNA extraction and storage using FTA card samples was investigated. New DNA sequences were generated using both DNA extractions from freshly collected material (116 sequences) and FTA card samples (115 sequences) for 100 taxa within Pyrenulaceae. Inferences using three ribosomal genes (nuLSU, mtSSU and ITS) highlighted delimitation problems in few taxa, including the pantropical species P. mamillana and P. quassiicola. Issues related to generic delimitations were also confirmed, with Anthracothecium, Pyrgillus and Lithothelium nested within the species rich genus Pyrenula. Although further taxon and gene sampling will be required to fully revise species and generic concepts within this family, our data allowed us to describe two new species from Brazil (Lithothelium immersum and Pyrenula minutispora) and one from North America (Pyrenula reebiae).}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 25135
  AU  - Gueidan,Cecile 
  AU  - Aptroot,Andr? 
  AU  - Caceres,Marcela E.S.
  AU  - Binh,Nguyen Quoc
  T1  - Molecular phylogeny of the tropical lichen family Pyrenulaceae: contribution from dried herbarium specimens and FTA card samples
  PY  - 2016
  KW  - Anthracothecium
  KW  - DNA extraction
  KW  - Pyrenula
  KW  - multigene phylogeny
  KW  - Whatman FTA card
  UR  - http://dx.doi.org/10.1007/s11557-015-1154-8
  N2  - The Pyrenulaceae is one of the most common lichen families in tropical rainforests. These mostly lichenised ascomycete fungi are relatively well known, as identification tools are available for many members of the family, including the most species rich genus Pyrenula. However, despite the past boom in DNA sequence generation for most living organisms, molecular data is still mainly lacking for these crustose corticolous lichens, mainly because genomic DNA does not seem to preserve well after specimen desiccation. Because of lengthy custom procedures, newly collected specimens are therefore often too old for molecular work when they reach the laboratory. Here, a new method of DNA extraction and storage using FTA card samples was investigated. New DNA sequences were generated using both DNA extractions from freshly collected material (116 sequences) and FTA card samples (115 sequences) for 100 taxa within Pyrenulaceae. Inferences using three ribosomal genes (nuLSU, mtSSU and ITS) highlighted delimitation problems in few taxa, including the pantropical species P. mamillana and P. quassiicola. Issues related to generic delimitations were also confirmed, with Anthracothecium, Pyrgillus and Lithothelium nested within the species rich genus Pyrenula. Although further taxon and gene sampling will be required to fully revise species and generic concepts within this family, our data allowed us to describe two new species from Brazil (Lithothelium immersum and Pyrenula minutispora) and one from North America (Pyrenula reebiae).
  L3  - 10.1007/s11557-015-1154-8
  JF  - Mycological Progress
  VL  - 15
  IS  - 7
  ER  -