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Citation for Study 18612

Citation title: "Metagenome from a Spirulina digesting biogas reactor: analysis via binning of contigs and classification of short reads".

Study name: "Metagenome from a Spirulina digesting biogas reactor: analysis via binning of contigs and classification of short reads".

This study is part of submission 18612 (Status: Published).

Citation

Nolla-ardevol V., Peces M., Strous M., & Tegetmeyer H.E. 2015. Metagenome from a Spirulina digesting biogas reactor: analysis via binning of contigs and classification of short reads. BMC Microbiology, .

Authors

• Nolla-ardevol V.
• Peces M.
• Strous M.
• Tegetmeyer H.E.

Abstract

Background Anaerobic digestion is a biological process in which a consortium of microorganisms transforms a complex substrate into methane and carbon dioxide. A good understanding of the interactions between the populations that form this consortium can contribute to successful anaerobic digestion of the substrate. In this study we combine the analysis of the biogas production in a laboratory anaerobic digester fed with the microalgae Spirulina, a protein rich substrate, with the analysis of the metagenome of the consortium responsible for digestion, obtained by high-throughput DNA sequencing. The obtained metagenome was also compared with a metagenome from a full scale biogas plant fed with cellulose rich material. Results The optimal organic loading rate for the anaerobic digestion of Spirulina was determined to be 4.0 g Spirulina L -1 day -1 with a specific biogas production of 350 mL biogas g Spirulina -1 with a methane content of 68%. Firmicutes dominated the microbial consortium at 38% abundance followed by Bacteroidetes, Chloroflexi and Thermotogae. Euryarchaeota represented 3.5% of the total abundance. The most abundant organism (14.9%) was related to Tissierella, a bacterium known to use proteinaceous substrates for growth. Methanomicrobiales and Methanosarcinales dominated the archaeal community. Compared to the full scale cellulose-fed digesters, Pfam domains related to protein degradation were more frequently detected and Pfam domains related to cellulose degradation were less frequent in our sample. Conclusions The results presented in this study suggest that Spirulina is a suitable substrate for the production of biogas. The proteinaceous substrate appeared to have a selective impact on the bacterial community that performed anaerobic digestion. A direct influence of the substrate on the selection of specific methanogenic populations was not observed.

Keywords

Metagenome; Metagenome comparison; Spirulina; Biogas; Binning: Microbial community

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S18612
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref25373,
  	 author = {Vimac  Nolla-Ardevol and Miriam  Peces and Marc  Strous and Halina Elisabeth Tegetmeyer},
  	 title = {Metagenome from a Spirulina digesting biogas reactor: analysis via binning of contigs and classification of short reads},
  	 year = {2015},
  	 keywords = {Metagenome; Metagenome comparison; Spirulina; Biogas; Binning: Microbial community},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {BMC Microbiology},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Background
  Anaerobic digestion is a biological process in which a consortium of microorganisms transforms a complex substrate into methane and carbon dioxide. A good understanding of the interactions between the populations that form this consortium can contribute to successful anaerobic digestion of the substrate.
  
  In this study we combine the analysis of the biogas production in a laboratory anaerobic digester fed with the microalgae Spirulina, a protein rich substrate, with the analysis of the metagenome of the consortium responsible for digestion, obtained by high-throughput DNA sequencing. The obtained metagenome was also compared with a metagenome from a full scale biogas plant fed with cellulose rich material.
  
  Results
  The optimal organic loading rate for the anaerobic digestion of Spirulina was determined to be 4.0 g Spirulina L -1 day -1 with a specific biogas production of 350 mL biogas g Spirulina -1 with a methane content of 68%. 
  Firmicutes dominated the microbial consortium at 38% abundance followed by Bacteroidetes, Chloroflexi and Thermotogae. Euryarchaeota represented 3.5% of the total abundance. The most abundant organism (14.9%) was related to Tissierella, a bacterium known to use proteinaceous substrates for growth. Methanomicrobiales and Methanosarcinales dominated the archaeal community. Compared to the full scale cellulose-fed digesters, Pfam domains related to protein degradation were more frequently detected and Pfam domains related to cellulose degradation were less frequent in our sample.
  
  Conclusions
  The results presented in this study suggest that Spirulina is a suitable substrate for the production of biogas. The proteinaceous substrate appeared to have a selective impact on the bacterial community that performed anaerobic digestion. A direct influence of the substrate on the selection of specific methanogenic populations was not observed.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 25373
  AU  - Nolla-Ardevol,Vimac 
  AU  - Peces,Miriam 
  AU  - Strous,Marc 
  AU  - Tegetmeyer,Halina Elisabeth
  T1  - Metagenome from a Spirulina digesting biogas reactor: analysis via binning of contigs and classification of short reads
  PY  - 2015
  KW  - Metagenome; Metagenome comparison; Spirulina; Biogas; Binning: Microbial community
  UR  - http://dx.doi.org/
  N2  - Background
  Anaerobic digestion is a biological process in which a consortium of microorganisms transforms a complex substrate into methane and carbon dioxide. A good understanding of the interactions between the populations that form this consortium can contribute to successful anaerobic digestion of the substrate.
  
  In this study we combine the analysis of the biogas production in a laboratory anaerobic digester fed with the microalgae Spirulina, a protein rich substrate, with the analysis of the metagenome of the consortium responsible for digestion, obtained by high-throughput DNA sequencing. The obtained metagenome was also compared with a metagenome from a full scale biogas plant fed with cellulose rich material.
  
  Results
  The optimal organic loading rate for the anaerobic digestion of Spirulina was determined to be 4.0 g Spirulina L -1 day -1 with a specific biogas production of 350 mL biogas g Spirulina -1 with a methane content of 68%. 
  Firmicutes dominated the microbial consortium at 38% abundance followed by Bacteroidetes, Chloroflexi and Thermotogae. Euryarchaeota represented 3.5% of the total abundance. The most abundant organism (14.9%) was related to Tissierella, a bacterium known to use proteinaceous substrates for growth. Methanomicrobiales and Methanosarcinales dominated the archaeal community. Compared to the full scale cellulose-fed digesters, Pfam domains related to protein degradation were more frequently detected and Pfam domains related to cellulose degradation were less frequent in our sample.
  
  Conclusions
  The results presented in this study suggest that Spirulina is a suitable substrate for the production of biogas. The proteinaceous substrate appeared to have a selective impact on the bacterial community that performed anaerobic digestion. A direct influence of the substrate on the selection of specific methanogenic populations was not observed.
  L3  - 
  JF  - BMC Microbiology
  VL  - 
  IS  - 
  ER  -