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Citation for Study 18654

Citation title: "Auxin-mediated expression of a GH3 gene in relation to ontogenic state in Chestnut ".

Study name: "Auxin-mediated expression of a GH3 gene in relation to ontogenic state in Chestnut ".

This study is part of submission 18654 (Status: Published).

Citation

Vielba J.M., Varas E., Rico S., Covelo P., & Sanchez C. 2016. Auxin-mediated expression of a GH3 gene in relation to ontogenic state in Chestnut. Trees, .

Authors

• Vielba J.M.
• Varas E.
• Rico S.
• Covelo P.
• Sanchez C.

Abstract

A new auxin inducible gene isolated from chestnut microshoots was found to encode a protein belonging to group II of the Gretchen Hagen 3 (GH3) family. The gene was, therefore, named CsGH3-1. Predicted protein sequence analysis revealed the presence of conserved domains involved in the conjugation of amino acids to indole-3-acetic acid (IAA). Modelling of the protein and molecular docking of IAA, indole-3-butyric-acid (IBA), 1-naphthaleneacetic acid (NAA), and benzothiazole-2-oxyacetic acid (BTOA) into the active site of CsGH3-1 indicated a high and similar binding affinity for the four substrates. Expression analysis by qPCR indicated that CsGH3-1 is regulated by wounding, darkness, and auxin in chestnut microshoots, in an ontogenetic-dependent manner. Under IBA treatment, upregulation of CsGH3-1 was higher in mature than in juvenile shoots and was negatively correlated with the ability of microshoots to form roots. High levels of auxin-induced expression of CsGH3-1 were detected in mature shoots 24 h after the IBA treatment, whereas transcript levels decreased in rooting-competent shoots with cell-type-specific expression. CsGH3-1 transcripts were specifically localized in cells involved in the initiation of adventitious roots only in rooting-competent shoots at the time when cells switch their fate to root initial cells. Thus, these data show a correlation between the specific localization of transcripts and rooting competence, and they also suggest a role for CsGH3-1 in regulating auxin homeostasis during the onset of adventitious root formation.

Keywords

adventitious roots, auxin, chestnut, GH3, molecular docking, ontogenic state.

External links

• DOI: 10.1007/s00468-016-1449-7

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S18654
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref25401,
  	 author = {Jes? M. Vielba and Elena  Varas and Saleta  Rico and Puri  Covelo and Conchi  Sanchez},
  	 title = {Auxin-mediated expression of a GH3 gene in relation to ontogenic state in Chestnut  },
  	 year = {2016},
  	 keywords = {adventitious roots, auxin, chestnut, GH3, molecular docking, ontogenic state.},
  	 doi = {10.1007/s00468-016-1449-7},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Trees},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {A new auxin inducible gene isolated from chestnut microshoots was found to encode a protein belonging to group II of the Gretchen Hagen 3 (GH3) family. The gene was, therefore, named CsGH3-1. Predicted protein sequence analysis revealed the presence of conserved domains involved in the conjugation of amino acids to indole-3-acetic acid (IAA). Modelling of the protein and molecular docking of IAA, indole-3-butyric-acid (IBA), 1-naphthaleneacetic acid (NAA), and benzothiazole-2-oxyacetic acid (BTOA) into the active site of CsGH3-1 indicated a high and similar binding affinity for the four substrates. Expression analysis by qPCR indicated that CsGH3-1 is regulated by wounding, darkness, and auxin in chestnut microshoots, in an ontogenetic-dependent manner. Under IBA treatment, upregulation of CsGH3-1 was higher in mature than in juvenile shoots and was negatively correlated with the ability of microshoots to form roots. High levels of auxin-induced expression of CsGH3-1 were detected in mature shoots 24 h after the IBA treatment, whereas transcript levels decreased in rooting-competent shoots with cell-type-specific expression. CsGH3-1 transcripts were specifically localized in cells involved in the initiation of adventitious roots only in rooting-competent shoots at the time when cells switch their fate to root initial cells. Thus, these data show a correlation between the specific localization of transcripts and rooting competence, and they also suggest a role for CsGH3-1 in regulating auxin homeostasis during the onset of adventitious root formation.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 25401
  AU  - Vielba,Jes? M.
  AU  - Varas,Elena 
  AU  - Rico,Saleta 
  AU  - Covelo,Puri 
  AU  - Sanchez,Conchi 
  T1  - Auxin-mediated expression of a GH3 gene in relation to ontogenic state in Chestnut  
  PY  - 2016
  KW  - adventitious roots
  KW  - auxin
  KW  - chestnut
  KW  - GH3
  KW  - molecular docking
  KW  - ontogenic state.
  UR  - http://dx.doi.org/10.1007/s00468-016-1449-7
  N2  - A new auxin inducible gene isolated from chestnut microshoots was found to encode a protein belonging to group II of the Gretchen Hagen 3 (GH3) family. The gene was, therefore, named CsGH3-1. Predicted protein sequence analysis revealed the presence of conserved domains involved in the conjugation of amino acids to indole-3-acetic acid (IAA). Modelling of the protein and molecular docking of IAA, indole-3-butyric-acid (IBA), 1-naphthaleneacetic acid (NAA), and benzothiazole-2-oxyacetic acid (BTOA) into the active site of CsGH3-1 indicated a high and similar binding affinity for the four substrates. Expression analysis by qPCR indicated that CsGH3-1 is regulated by wounding, darkness, and auxin in chestnut microshoots, in an ontogenetic-dependent manner. Under IBA treatment, upregulation of CsGH3-1 was higher in mature than in juvenile shoots and was negatively correlated with the ability of microshoots to form roots. High levels of auxin-induced expression of CsGH3-1 were detected in mature shoots 24 h after the IBA treatment, whereas transcript levels decreased in rooting-competent shoots with cell-type-specific expression. CsGH3-1 transcripts were specifically localized in cells involved in the initiation of adventitious roots only in rooting-competent shoots at the time when cells switch their fate to root initial cells. Thus, these data show a correlation between the specific localization of transcripts and rooting competence, and they also suggest a role for CsGH3-1 in regulating auxin homeostasis during the onset of adventitious root formation.
  L3  - 10.1007/s00468-016-1449-7
  JF  - Trees
  VL  - 
  IS  - 
  ER  -