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Citation for Study 18752

Citation title: "Complete Genome of Staphylococcus aureus Tager 104 Provides Evidence of Its Relation to Modern Systemic Hospital-Acquired Strains".

Study name: "Complete Genome of Staphylococcus aureus Tager 104 Provides Evidence of Its Relation to Modern Systemic Hospital-Acquired Strains".

This study is part of submission 18752 (Status: Published).

Citation

Davis iv R.W., Brannen A.D., Hossain M.J., Monsma S., Bock P.E., Nahrendorf M., Mead D.A., Lodes M., Liles M.R., & Panizzi P. 2016. Complete Genome of Staphylococcus aureus Tager 104 Provides Evidence of Its Relation to Modern Systemic Hospital-Acquired Strains. BioMed Central Genomics, .

Authors

• Davis iv R.W.
• Brannen A.D.
• Hossain M.J.
• Monsma S.
• Bock P.E.
• Nahrendorf M.
• Mead D.A.
• Lodes M.
• Liles M.R.
• Panizzi P. (submitter) 6154966828

Abstract

Background: Staphylococcus aureus (S. aureus) infections range in severity due to expression of certain virulence factors encoded on mobile genetic elements (MGE). As such, characterization of these MGE, as well as single nucleotide polymorphisms is of high clinical and microbiological importance. To understand the evolution of these dangerous pathogens, it is paramount to define reference strains that may predate MGE acquisition. One such candidate is S. aureus Tager 104, a previously uncharacterized strain isolated from a patient with impetigo in 1947. Results: We show here that S. aureus Tager 104 can survive in the bloodstream and infect na?ve organs. We also demonstrate a procedure to construct and validate the assembly of S. aureus genomes, using Tager 104 as a proof-of-concept. In so doing, we bridged confounding gap regions that limited our initial attempts to close this 2.82 Mb genome, through integration of data from Illumina Nextera paired-end, PacBio RS, and Lucigen NxSeq mate-pair libraries. Furthermore, we provide independent confirmation of our segmental arrangement of the Tager 104 genome by the sole use of Lucigen NxSeq libraries filled by paired-end MiSeq reads and alignment with SPAdes software. Genomic analysis of Tager 104 revealed limited MGE, and a νSaβ island configuration that is reminiscent of other hospital acquired S. aureus genomes. Conclusions: Tager 104 represents an early-branching ancestor of certain hospital-acquired strains. Combined with its earlier isolation date and limited content of MGE, Tager 104 can serve as a viable reference for future comparative genome studies.

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S18752
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref25553,
  	 author = {Richard W Davis IV and Andrew David Brannen and Mohammad J Hossain and Scott  Monsma and Paul E Bock and Matthias  Nahrendorf and David A Mead and Michael  Lodes and Mark R Liles and Peter  Panizzi},
  	 title = {Complete Genome of Staphylococcus aureus Tager 104 Provides Evidence of Its Relation to Modern Systemic Hospital-Acquired Strains},
  	 year = {2016},
  	 keywords = {},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {BioMed Central Genomics},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Background:  Staphylococcus aureus (S. aureus) infections range in severity due to expression of certain virulence factors encoded on mobile genetic elements (MGE).  As such, characterization of these MGE, as well as single nucleotide polymorphisms is of high clinical and microbiological importance. To understand the evolution of these dangerous pathogens, it is paramount to define reference strains that may predate MGE acquisition.  One such candidate is S. aureus Tager 104, a previously uncharacterized strain isolated from a patient with impetigo in 1947. 
  Results:  We show here that S. aureus Tager 104 can survive in the bloodstream and infect na?ve organs.  We also demonstrate a procedure to construct and validate the assembly of S. aureus genomes, using Tager 104 as a proof-of-concept.  In so doing, we bridged confounding gap regions that limited our initial attempts to close this 2.82 Mb genome, through integration of data from Illumina Nextera paired-end, PacBio RS, and Lucigen NxSeq mate-pair libraries.  Furthermore, we provide independent confirmation of our segmental arrangement of the Tager 104 genome by the sole use of Lucigen NxSeq libraries filled by paired-end MiSeq reads and alignment with SPAdes software.  Genomic analysis of Tager 104 revealed limited MGE, and a νSaβ island configuration that is reminiscent of other hospital acquired S. aureus genomes.  
  Conclusions:  Tager 104 represents an early-branching ancestor of certain hospital-acquired strains.  Combined with its earlier isolation date and limited content of MGE, Tager 104 can serve as a viable reference for future comparative genome studies.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 25553
  AU  - Davis IV,Richard W
  AU  - Brannen,Andrew David
  AU  - Hossain,Mohammad J
  AU  - Monsma,Scott 
  AU  - Bock,Paul E
  AU  - Nahrendorf,Matthias 
  AU  - Mead,David A
  AU  - Lodes,Michael 
  AU  - Liles,Mark R
  AU  - Panizzi,Peter 
  T1  - Complete Genome of Staphylococcus aureus Tager 104 Provides Evidence of Its Relation to Modern Systemic Hospital-Acquired Strains
  PY  - 2016
  KW  - 
  UR  - http://dx.doi.org/
  N2  - Background:  Staphylococcus aureus (S. aureus) infections range in severity due to expression of certain virulence factors encoded on mobile genetic elements (MGE).  As such, characterization of these MGE, as well as single nucleotide polymorphisms is of high clinical and microbiological importance. To understand the evolution of these dangerous pathogens, it is paramount to define reference strains that may predate MGE acquisition.  One such candidate is S. aureus Tager 104, a previously uncharacterized strain isolated from a patient with impetigo in 1947. 
  Results:  We show here that S. aureus Tager 104 can survive in the bloodstream and infect na?ve organs.  We also demonstrate a procedure to construct and validate the assembly of S. aureus genomes, using Tager 104 as a proof-of-concept.  In so doing, we bridged confounding gap regions that limited our initial attempts to close this 2.82 Mb genome, through integration of data from Illumina Nextera paired-end, PacBio RS, and Lucigen NxSeq mate-pair libraries.  Furthermore, we provide independent confirmation of our segmental arrangement of the Tager 104 genome by the sole use of Lucigen NxSeq libraries filled by paired-end MiSeq reads and alignment with SPAdes software.  Genomic analysis of Tager 104 revealed limited MGE, and a νSaβ island configuration that is reminiscent of other hospital acquired S. aureus genomes.  
  Conclusions:  Tager 104 represents an early-branching ancestor of certain hospital-acquired strains.  Combined with its earlier isolation date and limited content of MGE, Tager 104 can serve as a viable reference for future comparative genome studies.
  L3  - 
  JF  - BioMed Central Genomics
  VL  - 
  IS  - 
  ER  -