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Citation for Study 18840

Citation title: "Muscodor sp. endophyte from Citrus sinensis (Brazil): morphology, molecular characterization, and production of volatile compounds against Phyllosticta citricarpa".

Study name: "Muscodor sp. endophyte from Citrus sinensis (Brazil): morphology, molecular characterization, and production of volatile compounds against Phyllosticta citricarpa".

This study is part of submission 18840 (Status: Published).

Citation

Pena L.C., Jung L.F., Savi D.C., Servienski A., Aluizio R., Goulin E.H., Galli-terasawa L.V., Sales B.M., Annies V., Franco C.R., Glienke C., & Kava V. 2016. Muscodor sp. endophyte from Citrus sinensis (Brazil): morphology, molecular characterization, and production of volatile compounds against Phyllosticta citricarpa. Fungal Biology (presubmission), .

Authors

• Pena L.C. (submitter)
• Jung L.F.
• Savi D.C.
• Servienski A.
• Aluizio R. +554133611731
• Goulin E.H. 00554199322095
• Galli-terasawa L.V.
• Sales B.M.
• Annies V.
• Franco C.R.
• Glienke C.
• Kava V.

Abstract

The citrus industry is among the most important in the world. However, numerous diseases are known to affect this family of plants. One such disease is the Citrus Black Spot (CBS) caused by the fungus Phyllosticta citricarpa. To identify alternatives for the biological control of citrus diseases, endophytic fungi were isolated from healthy plants of the orchards with incidence of Citrus Black Spot, in Brazil. A collection of over 400 fungal isolates was obtained and among these isolates, the strain LGMF1254 was selected, because it inhibited P. citricarpa. This strain was identified as Muscodor sp. by morphologic and phylogenetic analysis, performed through ITS sequencing of rDNA. LGMF1254 showed high similarity with M. sutura, M. vitigenus, and M. equiseti; however, 10 polymorphic sites were observed on RPB2 sequences of M. sutura and LGMF1254 (the RPB2 sequences from M. vitigenus and M. equiseti are not available). By analyses of RPB2 sequence and volatile organic compounds (VOCs) production, LGMF1254 can be probably classified as a new species; however, sequences of genes other than ITS region are necessary to understand this genus better. LGMF1254 showed high activity against P. citricarpa, such as the inhibition of growth and pycnidia formation, due to the production of volatile secondary metabolites. These volatiles, among which the main products are thujopsene, cedrene, azulene, cis-α-bergamotene, and α-himachalene, could be used during the transport of fruits, preventing the development of the CBS symptoms. Here, for the first time in Brazil, we describe the isolation of Muscodor, as well the production of compounds with activity against the phytopathogen P. citricarpa from it.

Keywords

Key words: Muscodor, antagonism, volatile organic compounds, ITS rDNA, RPB2, citrus industry

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S18840
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref25534,
  	 author = {Lorena Carolina Pena and Luiz Fernando Jung and Daiani Cristina Savi and Andr?  Servienski and Rodrigo  Aluizio and Eduardo Henrique Goulin and Lygia  Vit?ria Galli-Terasawa and Beatriz Maia, B. H. L. N Sales and Vin?cius  Annies and Celia Regina Franco and Chirlei  Glienke and Vanessa  Kava},
  	 title = {Muscodor sp. endophyte from Citrus sinensis (Brazil): morphology, molecular characterization, and production of volatile compounds against  Phyllosticta citricarpa},
  	 year = {2016},
  	 keywords = {Key words: Muscodor, antagonism, volatile organic compounds, ITS rDNA, RPB2, citrus industry},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Fungal Biology (presubmission)},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {The citrus industry is among the most important in the world. However, numerous diseases are known to affect this family of plants. One such disease is the Citrus Black Spot (CBS) caused by the fungus Phyllosticta citricarpa. To identify alternatives for the biological control of citrus diseases, endophytic fungi were isolated from healthy plants of the orchards with incidence of Citrus Black Spot, in Brazil. A collection of over 400 fungal isolates was obtained and among these isolates, the strain LGMF1254 was selected, because it inhibited P. citricarpa. This strain was identified as Muscodor sp. by morphologic and phylogenetic analysis, performed through ITS sequencing of rDNA. LGMF1254 showed high similarity with M. sutura, M. vitigenus, and M. equiseti; however, 10 polymorphic sites were observed on RPB2 sequences of M. sutura and LGMF1254 (the RPB2 sequences from M. vitigenus and M. equiseti are not available). By analyses of RPB2 sequence and volatile organic compounds (VOCs) production, LGMF1254 can be probably classified as a new species; however, sequences of genes other than ITS region are necessary to understand this genus better. LGMF1254 showed high activity against P. citricarpa, such as the inhibition of growth and pycnidia formation, due to the production of volatile secondary metabolites. These volatiles, among which the main products are thujopsene, cedrene, azulene, cis-α-bergamotene, and α-himachalene, could be used during the transport of fruits, preventing the development of the CBS symptoms. Here, for the first time in Brazil, we describe the isolation of Muscodor, as well the production of compounds with activity against the phytopathogen P. citricarpa from it. }
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 25534
  AU  - Pena,Lorena Carolina
  AU  - Jung,Luiz Fernando
  AU  - Savi,Daiani Cristina
  AU  - Servienski,Andr? 
  AU  - Aluizio,Rodrigo 
  AU  - Goulin,Eduardo Henrique
  AU  - Galli-Terasawa,Lygia  Vit?ria
  AU  - Sales,Beatriz Maia, B. H. L. N
  AU  - Annies,Vin?cius 
  AU  - Franco,Celia Regina
  AU  - Glienke,Chirlei 
  AU  - Kava,Vanessa 
  T1  - Muscodor sp. endophyte from Citrus sinensis (Brazil): morphology, molecular characterization, and production of volatile compounds against  Phyllosticta citricarpa
  PY  - 2016
  KW  - Key words: Muscodor
  KW  - antagonism
  KW  - volatile organic compounds
  KW  - ITS rDNA
  KW  - RPB2
  KW  - citrus industry
  UR  - http://dx.doi.org/
  N2  - The citrus industry is among the most important in the world. However, numerous diseases are known to affect this family of plants. One such disease is the Citrus Black Spot (CBS) caused by the fungus Phyllosticta citricarpa. To identify alternatives for the biological control of citrus diseases, endophytic fungi were isolated from healthy plants of the orchards with incidence of Citrus Black Spot, in Brazil. A collection of over 400 fungal isolates was obtained and among these isolates, the strain LGMF1254 was selected, because it inhibited P. citricarpa. This strain was identified as Muscodor sp. by morphologic and phylogenetic analysis, performed through ITS sequencing of rDNA. LGMF1254 showed high similarity with M. sutura, M. vitigenus, and M. equiseti; however, 10 polymorphic sites were observed on RPB2 sequences of M. sutura and LGMF1254 (the RPB2 sequences from M. vitigenus and M. equiseti are not available). By analyses of RPB2 sequence and volatile organic compounds (VOCs) production, LGMF1254 can be probably classified as a new species; however, sequences of genes other than ITS region are necessary to understand this genus better. LGMF1254 showed high activity against P. citricarpa, such as the inhibition of growth and pycnidia formation, due to the production of volatile secondary metabolites. These volatiles, among which the main products are thujopsene, cedrene, azulene, cis-α-bergamotene, and α-himachalene, could be used during the transport of fruits, preventing the development of the CBS symptoms. Here, for the first time in Brazil, we describe the isolation of Muscodor, as well the production of compounds with activity against the phytopathogen P. citricarpa from it. 
  L3  - 
  JF  - Fungal Biology (presubmission)
  VL  - 
  IS  - 
  ER  -