CiteULike

Delicious

Connotea

Citation for Study 18980

Citation title: "Fusarium agapanthi sp. nov, a novel bikaverin and fusarubin-producing leaf and stem spot pathogen of Agapanthus praecox (African lily) from Australia and Italy".

Study name: "Fusarium agapanthi sp. nov, a novel bikaverin and fusarubin-producing leaf and stem spot pathogen of Agapanthus praecox (African lily) from Australia and Italy".

This study is part of submission 18980 (Status: Published).

Citation

Edwards J., Auer D., De alwis S., Summerell B., Aoki T., Proctor R.H., Busman M., & O'donnell K. 2016. Fusarium agapanthi sp. nov, a novel bikaverin and fusarubin-producing leaf and stem spot pathogen of Agapanthus praecox (African lily) from Australia and Italy. Mycologia , .

Authors

• Edwards J.
• Auer D.
• De alwis S.
• Summerell B.
• Aoki T.
• Proctor R.H.
• Busman M.
• O'donnell K. 309-681-6383

Abstract

This study was conducted to characterize a novel Fusarium species that caused leaf and stem spot on Agapanthus praecox (Agapanthus, African lily) in northern Italy and leaf rot and spot on the same host in Melbourne, Australia. Formally described here as Fusarium agapanthi, this novel pathogen was analyzed using phenotypic, phytopathogenic, secondary metabolite, molecular phylogenetic and genomic data. Five strains were characterized in the present study, including one isolated from symptomatic A. praecox in Saluzzo, Italy in 1999 and four from diseased leaf tissue from the same host exhibiting leaf rot and spot symptoms in the Melbourne Gardens, Royal Botanic Gardens Victoria, Australia in 2010. Maximum parsimony and maximum likelihood molecular phylogenetic analyses of portions of six individual genes and the combined dataset all strongly supported F. agapanthi either as the earliest diverging genealogically exclusive lineage within the American Clade of the F. fujikuroi species complex, or alternatively a novel monotypic lineage that is sister to the American Clade. Koch's postulates were completed on dwarf blue- and large white-flowering varieties of A. praecox, where two isolates of F. agapanthi were able to produce slowly spreading necrotic lesions when inoculated onto leaves and flower stems. Fusarium agapanthi can be distinguished from other fusaria by the production of densely branching aerial conidiophores with proliferating polyphialides that are produced throughout the aerial mycelium on synthetic nutrient-poor agar. BLASTn searches of the F. agapanthi NRRL 31653 and NRRL 54464 (= VPRI 41787) genome sequences were conducted to predict sexual reproductive mode and mycotoxin potential. Results of the BLASTn searches indicated that they possessed MAT1-2 and MAT1-1 idiomorphs, respectively, indicating that this species might possess a heterothallic sexual reproductive mode. Furthermore, based on the discovery of homologs of the bikaverin and fusarubin biosynthetic gene clusters in their genomes, liquid chromatography and tandem mass spectrometry analyses were conducted that confirmed production of these secondary metabolites on rice and corn kernel cultures.

Keywords

Fusarium fujikuroi species complex, GCPSR ? genealogical concordance phylogenetic species recognition, gene genealogies, mating type, molecular phylogenetics, morphology, mycotoxins, secondary metabolites

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S18980
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref25643,
  	 author = {Jacqueline  Edwards and Desmond  Auer and Sri-Kanthi   de Alwis and Bret A.  Summerell and Takayuki  Aoki and Robert H. Proctor and Mark  Busman and Kerry  O'Donnell},
  	 title = {Fusarium agapanthi sp. nov, a novel bikaverin and fusarubin-producing leaf and stem spot pathogen of Agapanthus praecox (African lily) from Australia and Italy},
  	 year = {2016},
  	 keywords = {Fusarium fujikuroi species complex, GCPSR ? genealogical concordance phylogenetic species recognition, gene genealogies, mating type, molecular phylogenetics, morphology, mycotoxins, secondary metabolites},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Mycologia },
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {This study was conducted to characterize a novel Fusarium species that caused leaf and stem spot on Agapanthus praecox (Agapanthus, African lily) in northern Italy and leaf rot and spot on the same host in Melbourne, Australia.  Formally described here as Fusarium agapanthi, this novel pathogen was analyzed using phenotypic, phytopathogenic, secondary metabolite, molecular phylogenetic and genomic data.  Five strains were characterized in the present study, including one isolated from symptomatic A. praecox in Saluzzo, Italy in 1999 and four from diseased leaf tissue from the same host exhibiting leaf rot and spot symptoms in the Melbourne Gardens, Royal Botanic Gardens Victoria, Australia in 2010.  Maximum parsimony and maximum likelihood molecular phylogenetic analyses of portions of six individual genes and the combined dataset all strongly supported F. agapanthi either as the earliest diverging genealogically exclusive lineage within the American Clade of the F. fujikuroi species complex, or alternatively a novel monotypic lineage that is sister to the American Clade.  Koch's postulates were completed on dwarf blue- and large white-flowering varieties of A. praecox, where two isolates of F. agapanthi were able to produce slowly spreading necrotic lesions when inoculated onto leaves and flower stems.  Fusarium agapanthi can be distinguished from other fusaria by the production of densely branching aerial conidiophores with proliferating polyphialides that are produced throughout the aerial mycelium on synthetic nutrient-poor agar.  BLASTn searches of the F. agapanthi NRRL 31653 and NRRL 54464 (= VPRI 41787) genome sequences were conducted to predict sexual reproductive mode and mycotoxin potential. Results of the BLASTn searches indicated that they possessed MAT1-2 and MAT1-1 idiomorphs, respectively, indicating that this species might possess a heterothallic sexual reproductive mode.  Furthermore, based on the discovery of homologs of the bikaverin and fusarubin biosynthetic gene clusters in their genomes, liquid chromatography and tandem mass spectrometry analyses were conducted that confirmed production of these secondary metabolites on rice and corn kernel cultures.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 25643
  AU  - Edwards,Jacqueline 
  AU  - Auer,Desmond 
  AU  - de Alwis,Sri-Kanthi  
  AU  - Summerell,Bret A. 
  AU  - Aoki,Takayuki 
  AU  - Proctor,Robert H.
  AU  - Busman,Mark 
  AU  - O'Donnell,Kerry 
  T1  - Fusarium agapanthi sp. nov, a novel bikaverin and fusarubin-producing leaf and stem spot pathogen of Agapanthus praecox (African lily) from Australia and Italy
  PY  - 2016
  KW  - Fusarium fujikuroi species complex
  KW  - GCPSR ? genealogical concordance phylogenetic species recognition
  KW  - gene genealogies
  KW  - mating type
  KW  - molecular phylogenetics
  KW  - morphology
  KW  - mycotoxins
  KW  - secondary metabolites
  UR  - http://dx.doi.org/
  N2  - This study was conducted to characterize a novel Fusarium species that caused leaf and stem spot on Agapanthus praecox (Agapanthus, African lily) in northern Italy and leaf rot and spot on the same host in Melbourne, Australia.  Formally described here as Fusarium agapanthi, this novel pathogen was analyzed using phenotypic, phytopathogenic, secondary metabolite, molecular phylogenetic and genomic data.  Five strains were characterized in the present study, including one isolated from symptomatic A. praecox in Saluzzo, Italy in 1999 and four from diseased leaf tissue from the same host exhibiting leaf rot and spot symptoms in the Melbourne Gardens, Royal Botanic Gardens Victoria, Australia in 2010.  Maximum parsimony and maximum likelihood molecular phylogenetic analyses of portions of six individual genes and the combined dataset all strongly supported F. agapanthi either as the earliest diverging genealogically exclusive lineage within the American Clade of the F. fujikuroi species complex, or alternatively a novel monotypic lineage that is sister to the American Clade.  Koch's postulates were completed on dwarf blue- and large white-flowering varieties of A. praecox, where two isolates of F. agapanthi were able to produce slowly spreading necrotic lesions when inoculated onto leaves and flower stems.  Fusarium agapanthi can be distinguished from other fusaria by the production of densely branching aerial conidiophores with proliferating polyphialides that are produced throughout the aerial mycelium on synthetic nutrient-poor agar.  BLASTn searches of the F. agapanthi NRRL 31653 and NRRL 54464 (= VPRI 41787) genome sequences were conducted to predict sexual reproductive mode and mycotoxin potential. Results of the BLASTn searches indicated that they possessed MAT1-2 and MAT1-1 idiomorphs, respectively, indicating that this species might possess a heterothallic sexual reproductive mode.  Furthermore, based on the discovery of homologs of the bikaverin and fusarubin biosynthetic gene clusters in their genomes, liquid chromatography and tandem mass spectrometry analyses were conducted that confirmed production of these secondary metabolites on rice and corn kernel cultures.
  L3  - 
  JF  - Mycologia 
  VL  - 
  IS  - 
  ER  -