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Citation for Study 19755

Citation title: "Genomic and metabolic characterisation of alkaloid biosynthesis by asexual Epichlo? fungal endophytes of tall fescue pasture grasses".

Study name: "Genomic and metabolic characterisation of alkaloid biosynthesis by asexual Epichlo? fungal endophytes of tall fescue pasture grasses".

This study is part of submission 19755 (Status: Published).

Citation

Ekanayake P.N., Kaur J., Tian P., Rochfort S.J., Guthridge K.M., Sawbridge T.I., Spangenberg G.C., & Forster J.W. 2016. Genomic and metabolic characterisation of alkaloid biosynthesis by asexual Epichlo? fungal endophytes of tall fescue pasture grasses. Genome, .

Authors

• Ekanayake P.N.
• Kaur J.
• Tian P.
• Rochfort S.J.
• Guthridge K.M.
• Sawbridge T.I.
• Spangenberg G.C.
• Forster J.W.

Abstract

Symbiotic associations between tall fescue grasses and asexual Epichlo? fungal endophytes are characterised by biosynthesis of alkaloid compounds causing both beneficial (protection against invertebrate herbivory) and detrimental (livestock toxicosis) effects. The former category includes peramine and lolines, and the latter includes lolitrem B and ergovaline. Candidate novel endophytes with favourable chemotypic profiles have been identified in germplasm collections by screening for genetic diversity, followed by metabolite profile analysis in endogenous genetic backgrounds. A sub-set of candidates was subjected to genome survey sequencing to detect the presence or absence and structural status of known genes in the biosynthetic pathways of the major alkaloid classes. The capacity to produce specific metabolites was directly predictable from metabolic data. In addition, study of duplicated gene structure in heteroploid genomic constitutions provided additional evidence for the origin of such endophytes. Selected strains were inoculated into meristem-derived callus cultures from specific tall fescue genotypes in order to perform isogenic comparisons of alkaloid profile in different host backgrounds. This process revealed evidence for host-specific quantitative control of metabolite production, consistent with previous studies. Certain strains were capable of both inoculation and formation of longer-term associations with a non-host species, perennial ryegrass. A process based on discovery and primary characterisation of novel endophytes based on DNA analysis, followed by confirmatory metabolic studies, offers improvements of speed and efficiency and hence accelerated deployment in pasture grass improvement programs.

Keywords

Festuca, lolitrem B, loline, ergovaline, peramine, inoculation

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S19755
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref26237,
  	 author = {Piyumi  N Ekanayake and Jatinder  Kaur and Pei  Tian and Simone J Rochfort and Kathryn M Guthridge and Timothy I Sawbridge and German C Spangenberg and John W Forster},
  	 title = {Genomic and metabolic characterisation of alkaloid biosynthesis by asexual Epichlo? fungal endophytes of tall fescue pasture grasses},
  	 year = {2016},
  	 keywords = {Festuca, lolitrem B, loline, ergovaline, peramine, inoculation},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Genome},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Symbiotic associations between tall fescue grasses and asexual Epichlo? fungal endophytes are characterised by biosynthesis of alkaloid compounds causing both beneficial (protection against invertebrate herbivory) and detrimental (livestock toxicosis) effects. The former category includes peramine and lolines, and the latter includes lolitrem B and ergovaline. Candidate novel endophytes with favourable chemotypic profiles have been identified in germplasm collections by screening for genetic diversity, followed by metabolite profile analysis in endogenous genetic backgrounds. A sub-set of candidates was subjected to genome survey sequencing to detect the presence or absence and structural status of known genes in the biosynthetic pathways of the major alkaloid classes. The capacity to produce specific metabolites was directly predictable from metabolic data. In addition, study of duplicated gene structure in heteroploid genomic constitutions provided additional evidence for the origin of such endophytes. Selected strains were inoculated into meristem-derived callus cultures from specific tall fescue genotypes in order to perform isogenic comparisons of alkaloid profile in different host backgrounds. This process revealed evidence for host-specific quantitative control of metabolite production, consistent with previous studies. Certain strains were capable of both inoculation and formation of longer-term associations with a non-host species, perennial ryegrass. A process based on discovery and primary characterisation of novel endophytes based on DNA analysis, followed by confirmatory metabolic studies, offers improvements of speed and efficiency and hence accelerated deployment in pasture grass improvement programs.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 26237
  AU  - Ekanayake,Piyumi  N
  AU  - Kaur,Jatinder 
  AU  - Tian,Pei 
  AU  - Rochfort,Simone J
  AU  - Guthridge,Kathryn M
  AU  - Sawbridge,Timothy I
  AU  - Spangenberg,German C
  AU  - Forster,John W
  T1  - Genomic and metabolic characterisation of alkaloid biosynthesis by asexual Epichlo? fungal endophytes of tall fescue pasture grasses
  PY  - 2016
  KW  - Festuca
  KW  - lolitrem B
  KW  - loline
  KW  - ergovaline
  KW  - peramine
  KW  - inoculation
  UR  - http://dx.doi.org/
  N2  - Symbiotic associations between tall fescue grasses and asexual Epichlo? fungal endophytes are characterised by biosynthesis of alkaloid compounds causing both beneficial (protection against invertebrate herbivory) and detrimental (livestock toxicosis) effects. The former category includes peramine and lolines, and the latter includes lolitrem B and ergovaline. Candidate novel endophytes with favourable chemotypic profiles have been identified in germplasm collections by screening for genetic diversity, followed by metabolite profile analysis in endogenous genetic backgrounds. A sub-set of candidates was subjected to genome survey sequencing to detect the presence or absence and structural status of known genes in the biosynthetic pathways of the major alkaloid classes. The capacity to produce specific metabolites was directly predictable from metabolic data. In addition, study of duplicated gene structure in heteroploid genomic constitutions provided additional evidence for the origin of such endophytes. Selected strains were inoculated into meristem-derived callus cultures from specific tall fescue genotypes in order to perform isogenic comparisons of alkaloid profile in different host backgrounds. This process revealed evidence for host-specific quantitative control of metabolite production, consistent with previous studies. Certain strains were capable of both inoculation and formation of longer-term associations with a non-host species, perennial ryegrass. A process based on discovery and primary characterisation of novel endophytes based on DNA analysis, followed by confirmatory metabolic studies, offers improvements of speed and efficiency and hence accelerated deployment in pasture grass improvement programs.
  L3  - 
  JF  - Genome
  VL  - 
  IS  - 
  ER  -