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Citation for Study 19762

Citation title: "Specificity in diversity ? single origin of a widespread ciliate-bacteria symbiosis".

Study name: "Specificity in diversity ? single origin of a widespread ciliate-bacteria symbiosis".

This study is part of submission 19762 (Status: Published).

Citation

Seah B.K., Schwaha T., Volland J., Huettel B., Dubilier N., & Gruber-vodicka H. 2017. Specificity in diversity ? single origin of a widespread ciliate-bacteria symbiosis. Proceedings of the Royal Society B: Biological Sciences, .

Authors

• Seah B.K. (submitter) +494212028904
• Schwaha T.
• Volland J.
• Huettel B.
• Dubilier N.
• Gruber-vodicka H.

Abstract

Symbioses between eukaryotes and sulphur-oxidizing (thiotrophic) bacteria have convergently evolved multiple times. Although well-described in at least eight classes of metazoan animals, almost nothing is known about the evolution of thiotrophic symbioses in microbial eukaryotes (protists). In this study, we characterized the symbioses between mouthless marine ciliates of the genus Kentrophoros, and their thiotrophic bacteria, using comparative sequence analysis and fluorescence in-situ hybridization. Ciliate small-subunit rRNA sequences were obtained from 17 morphospecies collected in the Mediterranean and Caribbean, and symbiont sequences from 13 of these morphospecies. We discovered a new Kentrophoros morphotype where the symbiont-bearing surface is folded into pouch-like compartments, illustrating the variability of the basic body plan. Phylogenetic analyses revealed that all investigated Kentrophoros belonged to a single clade, despite the remarkable morphological diversity of these hosts. The symbionts were also monophyletic and belonged to a new clade within the Gammaproteobacteria, with no known cultured representatives. Each host morphospecies had a distinct symbiont phylotype, and statistical analyses revealed significant support for host-symbiont codiversification. Given that these symbioses were collected from two widely-separated oceans, our results indicate that symbiotic associations in unicellular hosts can be highly specific and stable over long periods of evolutionary time.

Keywords

symbiosis, thiotrophy, ciliate, Gammaproteobacteria

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S19762
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref26244,
  	 author = {Brandon Kwee Boon Seah and Thomas  Schwaha and Jean-Marie  Volland and Bruno  Huettel and Nicole  Dubilier and Harald  Gruber-Vodicka},
  	 title = {Specificity in diversity ? single origin of a widespread ciliate-bacteria symbiosis},
  	 year = {2017},
  	 keywords = {symbiosis, thiotrophy, ciliate, Gammaproteobacteria},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Proceedings of the Royal Society B: Biological Sciences},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Symbioses between eukaryotes and sulphur-oxidizing (thiotrophic) bacteria have convergently evolved multiple times. Although well-described in at least eight classes of metazoan animals, almost nothing is known about the evolution of thiotrophic symbioses in microbial eukaryotes (protists). In this study, we characterized the symbioses between mouthless marine ciliates of the genus Kentrophoros, and their thiotrophic bacteria, using comparative sequence analysis and fluorescence in-situ hybridization. Ciliate small-subunit rRNA sequences were obtained from 17 morphospecies collected in the Mediterranean and Caribbean, and symbiont sequences from 13 of these morphospecies. We discovered a new Kentrophoros morphotype where the symbiont-bearing surface is folded into pouch-like compartments, illustrating the variability of the basic body plan. Phylogenetic analyses revealed that all investigated Kentrophoros belonged to a single clade, despite the remarkable morphological diversity of these hosts. The symbionts were also monophyletic and belonged to a new clade within the Gammaproteobacteria, with no known cultured representatives. Each host morphospecies had a distinct symbiont phylotype, and statistical analyses revealed significant support for host-symbiont codiversification. Given that these symbioses were collected from two widely-separated oceans, our results indicate that symbiotic associations in unicellular hosts can be highly specific and stable over long periods of evolutionary time.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 26244
  AU  - Seah,Brandon Kwee Boon
  AU  - Schwaha,Thomas 
  AU  - Volland,Jean-Marie 
  AU  - Huettel,Bruno 
  AU  - Dubilier,Nicole 
  AU  - Gruber-Vodicka,Harald 
  T1  - Specificity in diversity ? single origin of a widespread ciliate-bacteria symbiosis
  PY  - 2017
  KW  - symbiosis
  KW  - thiotrophy
  KW  - ciliate
  KW  - Gammaproteobacteria
  UR  - http://dx.doi.org/
  N2  - Symbioses between eukaryotes and sulphur-oxidizing (thiotrophic) bacteria have convergently evolved multiple times. Although well-described in at least eight classes of metazoan animals, almost nothing is known about the evolution of thiotrophic symbioses in microbial eukaryotes (protists). In this study, we characterized the symbioses between mouthless marine ciliates of the genus Kentrophoros, and their thiotrophic bacteria, using comparative sequence analysis and fluorescence in-situ hybridization. Ciliate small-subunit rRNA sequences were obtained from 17 morphospecies collected in the Mediterranean and Caribbean, and symbiont sequences from 13 of these morphospecies. We discovered a new Kentrophoros morphotype where the symbiont-bearing surface is folded into pouch-like compartments, illustrating the variability of the basic body plan. Phylogenetic analyses revealed that all investigated Kentrophoros belonged to a single clade, despite the remarkable morphological diversity of these hosts. The symbionts were also monophyletic and belonged to a new clade within the Gammaproteobacteria, with no known cultured representatives. Each host morphospecies had a distinct symbiont phylotype, and statistical analyses revealed significant support for host-symbiont codiversification. Given that these symbioses were collected from two widely-separated oceans, our results indicate that symbiotic associations in unicellular hosts can be highly specific and stable over long periods of evolutionary time.
  L3  - 
  JF  - Proceedings of the Royal Society B: Biological Sciences
  VL  - 
  IS  - 
  ER  -