@ARTICLE{TreeBASE2Ref26682,
author = {Seetha Jaganathan King and Peck Toung Ooi and Lai Yee Phang and Zeenathul Nazariah Binti Allaudin and Wei Hoong Loh and Chiou Yan Tee and Shiao Pau How and Karen Yip and Raymond Choo and Ban Keong Lim},
title = {Phylogenetic Characterization of genes encoding for viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of Porcine Reproductive & Respiratory Syndrome Virus found in Malaysia in 2013 and 2014},
year = {2016},
keywords = {Porcine reproductive and respiratory syndrome virus, PRRSV, genetic characterization, ORF5 gene, ORF7 gene},
doi = {},
url = {http://},
pmid = {},
journal = {BMC Veterinary Research},
volume = {},
number = {},
pages = {},
abstract = {Background:
Porcine reproductive and respiratory syndrome (PRRS) is one of the most expensive diseases of modern swine production & results in annual economic losses and cost the industry over 600 million USD in U.S alone and billions of dollars worldwide. Two atypical PRRS cases were observed in 2013 and 2014 characterized by late-term abortion, fever and sudden increase in sow mortality which persisted for a prolonged period of time.
Methods:
Lungs, lymph nodes and other samples were collected for disease investigation. Sequencing of the viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of PRRSV was done using the BigDye Terminator v3.1 cycle sequencing kit chemistry. The phylogenetic tree was constructed by using the Maximum Likelihood method, generated by Mega 6?.
Results:
Analysis of the ORF5 and ORF7 showed high degree of sequence homology to PRRSV parent vaccine strain VR-2332, RespPRRSV and other mutant/chimeric virus strains.
Conclusions:
Our study suggests that recombination events between vaccine strains and field isolates may contribute to PRRSV virulence in the field.
}
}
Citation for Study 20336
Citation title:
"Phylogenetic Characterization of genes encoding for viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of Porcine Reproductive & Respiratory Syndrome Virus found in Malaysia in 2013 and 2014".
Study name:
"Phylogenetic Characterization of genes encoding for viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of Porcine Reproductive & Respiratory Syndrome Virus found in Malaysia in 2013 and 2014".
This study is part of submission 20336
(Status: Published).
Citation
Jaganathan king S., Ooi P.T., Phang L.Y., Allaudin Z.B., Loh W.H., Tee C.Y., How S.P., Yip K., Choo R., & Lim B.K. 2016. Phylogenetic Characterization of genes encoding for viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of Porcine Reproductive & Respiratory Syndrome Virus found in Malaysia in 2013 and 2014. BMC Veterinary Research, .
Authors
-
Jaganathan king S.
(submitter)
+60126105879
-
Ooi P.T.
-
Phang L.Y.
-
Allaudin Z.B.
-
Loh W.H.
-
Tee C.Y.
-
How S.P.
-
Yip K.
-
Choo R.
-
Lim B.K.
Abstract
Background:
Porcine reproductive and respiratory syndrome (PRRS) is one of the most expensive diseases of modern swine production & results in annual economic losses and cost the industry over 600 million USD in U.S alone and billions of dollars worldwide. Two atypical PRRS cases were observed in 2013 and 2014 characterized by late-term abortion, fever and sudden increase in sow mortality which persisted for a prolonged period of time.
Methods:
Lungs, lymph nodes and other samples were collected for disease investigation. Sequencing of the viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of PRRSV was done using the BigDye Terminator v3.1 cycle sequencing kit chemistry. The phylogenetic tree was constructed by using the Maximum Likelihood method, generated by Mega 6?.
Results:
Analysis of the ORF5 and ORF7 showed high degree of sequence homology to PRRSV parent vaccine strain VR-2332, RespPRRSV and other mutant/chimeric virus strains.
Conclusions:
Our study suggests that recombination events between vaccine strains and field isolates may contribute to PRRSV virulence in the field.
Keywords
Porcine reproductive and respiratory syndrome virus, PRRSV, genetic characterization, ORF5 gene, ORF7 gene
External links
About this resource
- Canonical resource URI:
http://purl.org/phylo/treebase/phylows/study/TB2:S20336
- Other versions:
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NeXML
- Show BibTeX reference
@ARTICLE{TreeBASE2Ref26682,
author = {Seetha Jaganathan King and Peck Toung Ooi and Lai Yee Phang and Zeenathul Nazariah Binti Allaudin and Wei Hoong Loh and Chiou Yan Tee and Shiao Pau How and Karen Yip and Raymond Choo and Ban Keong Lim},
title = {Phylogenetic Characterization of genes encoding for viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of Porcine Reproductive & Respiratory Syndrome Virus found in Malaysia in 2013 and 2014},
year = {2016},
keywords = {Porcine reproductive and respiratory syndrome virus, PRRSV, genetic characterization, ORF5 gene, ORF7 gene},
doi = {},
url = {http://},
pmid = {},
journal = {BMC Veterinary Research},
volume = {},
number = {},
pages = {},
abstract = {Background:
Porcine reproductive and respiratory syndrome (PRRS) is one of the most expensive diseases of modern swine production & results in annual economic losses and cost the industry over 600 million USD in U.S alone and billions of dollars worldwide. Two atypical PRRS cases were observed in 2013 and 2014 characterized by late-term abortion, fever and sudden increase in sow mortality which persisted for a prolonged period of time.
Methods:
Lungs, lymph nodes and other samples were collected for disease investigation. Sequencing of the viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of PRRSV was done using the BigDye Terminator v3.1 cycle sequencing kit chemistry. The phylogenetic tree was constructed by using the Maximum Likelihood method, generated by Mega 6?.
Results:
Analysis of the ORF5 and ORF7 showed high degree of sequence homology to PRRSV parent vaccine strain VR-2332, RespPRRSV and other mutant/chimeric virus strains.
Conclusions:
Our study suggests that recombination events between vaccine strains and field isolates may contribute to PRRSV virulence in the field.
}
}
- Show RIS reference
TY - JOUR
ID - 26682
AU - Jaganathan King,Seetha
AU - Ooi,Peck Toung
AU - Phang,Lai Yee
AU - Allaudin,Zeenathul Nazariah Binti
AU - Loh,Wei Hoong
AU - Tee,Chiou Yan
AU - How,Shiao Pau
AU - Yip,Karen
AU - Choo,Raymond
AU - Lim,Ban Keong
T1 - Phylogenetic Characterization of genes encoding for viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of Porcine Reproductive & Respiratory Syndrome Virus found in Malaysia in 2013 and 2014
PY - 2016
KW - Porcine reproductive and respiratory syndrome virus
KW - PRRSV
KW - genetic characterization
KW - ORF5 gene
KW - ORF7 gene
UR - http://dx.doi.org/
N2 - Background:
Porcine reproductive and respiratory syndrome (PRRS) is one of the most expensive diseases of modern swine production & results in annual economic losses and cost the industry over 600 million USD in U.S alone and billions of dollars worldwide. Two atypical PRRS cases were observed in 2013 and 2014 characterized by late-term abortion, fever and sudden increase in sow mortality which persisted for a prolonged period of time.
Methods:
Lungs, lymph nodes and other samples were collected for disease investigation. Sequencing of the viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of PRRSV was done using the BigDye Terminator v3.1 cycle sequencing kit chemistry. The phylogenetic tree was constructed by using the Maximum Likelihood method, generated by Mega 6?.
Results:
Analysis of the ORF5 and ORF7 showed high degree of sequence homology to PRRSV parent vaccine strain VR-2332, RespPRRSV and other mutant/chimeric virus strains.
Conclusions:
Our study suggests that recombination events between vaccine strains and field isolates may contribute to PRRSV virulence in the field.
L3 -
JF - BMC Veterinary Research
VL -
IS -
ER -
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