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Citation for Study 20495

Citation title: "Schizophyllum radiatum, an Emerging Fungus from Human Respiratory Tract".

Study name: "Schizophyllum radiatum, an Emerging Fungus from Human Respiratory Tract".

This study is part of submission 20495 (Status: Published).

Citation

Siqueira J.P., Sutton D., Gene J., Garcia D., Guevara-suarez M., Decock C., Wiederhold N.P., & Guarro J. 2016. Schizophyllum radiatum, an Emerging Fungus from Human Respiratory Tract. Journal of Clinical Microbiology, 54(10): 2491-2497.

Authors

• Siqueira J.P.
• Sutton D.
• Gene J.
• Garcia D.
• Guevara-suarez M.
• Decock C.
• Wiederhold N.P.
• Guarro J.

Abstract

Schizophyllum is an important genus of basidiomycetes that, apart from being of genetic and biotechnological interest, is also reported to be a plant and animal pathogen. Schizophyllum commune is the best-known species and the only one reported from clinical specimens thus far, being recovered mainly from the respiratory tract. The aim of this study was to determine the species diversity of 23 clinical isolates of Schizophyllum from the United States using multilocus phylogenetic analysis and their in vitro susceptibilities to six drugs. The markers used for sequencing were the internal transcribed spacer (ITS), a portion of the nuclear large subunit (LSU) of ribosomal DNA, the RNA polymerase II second-largest subunit (RPB2), and the translation elongation factor 1 (EF-1) gene. The analyses revealed that 22 of the clinical isolates were in the Schizophyllum radiatum clade with high support values and 1 isolate was in the S. commune clade. This is the first report of this species in clinical samples. The two species mentioned above showed very similar morphological features in culture (i.e., white, cottony, unsporulated colonies composed of hyphae with clamp connections), making morphological discrimination between the two impossible. An epitype is designed for S. radiatum, and its sequences have been deposited in GenBank. The antifungal that showed the greatest in vitro activity against the strains tested was shown to be amphotericin B. In general, the strains of S. radiatum showed higher MICs than S. commune.

External links

• DOI: 10.1128/JCM.01170-16

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S20495
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref26803,
  	 author = {Joao Paulo Siqueira and Deanna A.   Sutton and Josepa  Gene and Dania  Garcia and Marcela  Guevara-Suarez and Cony  Decock and Nathan P Wiederhold and Josep  Guarro},
  	 title = {Schizophyllum radiatum, an Emerging Fungus from Human Respiratory Tract},
  	 year = {2016},
  	 keywords = {},
  	 doi = {10.1128/JCM.01170-16},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Journal of Clinical Microbiology},
  	 volume = {54},
  	 number = {10},
  	 pages = {2491--2497},
  	 abstract = {Schizophyllum is an important genus of basidiomycetes that, apart from being of genetic and biotechnological interest, is also reported to be a plant and animal pathogen. Schizophyllum commune is the best-known species and the only one reported from clinical specimens thus far, being recovered mainly from the respiratory tract. The aim of this study was to determine the species diversity of 23 clinical isolates of Schizophyllum from the United States using multilocus phylogenetic analysis and their in vitro susceptibilities to six drugs. The markers used for sequencing were the internal transcribed spacer (ITS), a portion of the nuclear large subunit (LSU) of ribosomal DNA, the RNA polymerase II second-largest subunit (RPB2), and the translation elongation factor 1 (EF-1) gene. The analyses revealed that 22 of the clinical isolates were in the Schizophyllum radiatum clade with high support values and 1 isolate was in the S. commune clade. This is the first report of this species in clinical samples. The two species mentioned above showed very similar morphological features in culture (i.e., white, cottony, unsporulated colonies composed of hyphae with clamp connections), making morphological discrimination between the two impossible. An epitype is designed for S. radiatum, and its sequences have been deposited in GenBank. The antifungal that showed the greatest in vitro activity against the strains tested was shown to be amphotericin B. In general, the strains of S. radiatum showed higher MICs than S. commune.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 26803
  AU  - Siqueira,Joao Paulo
  AU  - Sutton,Deanna A.  
  AU  - Gene,Josepa 
  AU  - Garcia,Dania 
  AU  - Guevara-Suarez,Marcela 
  AU  - Decock,Cony 
  AU  - Wiederhold,Nathan P
  AU  - Guarro,Josep 
  T1  - Schizophyllum radiatum, an Emerging Fungus from Human Respiratory Tract
  PY  - 2016
  KW  - 
  UR  - http://dx.doi.org/10.1128/JCM.01170-16
  N2  - Schizophyllum is an important genus of basidiomycetes that, apart from being of genetic and biotechnological interest, is also reported to be a plant and animal pathogen. Schizophyllum commune is the best-known species and the only one reported from clinical specimens thus far, being recovered mainly from the respiratory tract. The aim of this study was to determine the species diversity of 23 clinical isolates of Schizophyllum from the United States using multilocus phylogenetic analysis and their in vitro susceptibilities to six drugs. The markers used for sequencing were the internal transcribed spacer (ITS), a portion of the nuclear large subunit (LSU) of ribosomal DNA, the RNA polymerase II second-largest subunit (RPB2), and the translation elongation factor 1 (EF-1) gene. The analyses revealed that 22 of the clinical isolates were in the Schizophyllum radiatum clade with high support values and 1 isolate was in the S. commune clade. This is the first report of this species in clinical samples. The two species mentioned above showed very similar morphological features in culture (i.e., white, cottony, unsporulated colonies composed of hyphae with clamp connections), making morphological discrimination between the two impossible. An epitype is designed for S. radiatum, and its sequences have been deposited in GenBank. The antifungal that showed the greatest in vitro activity against the strains tested was shown to be amphotericin B. In general, the strains of S. radiatum showed higher MICs than S. commune.
  L3  - 10.1128/JCM.01170-16
  JF  - Journal of Clinical Microbiology
  VL  - 54
  IS  - 10
  SP  - 2491
  EP  - 2497
  ER  -