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Citation for Study 21138

Citation title: "Lack of S-RNase-based gametophytic self-incompatibility in orchids suggests this system evolved after the monocot-eudicot split".

Study name: "Lack of S-RNase-based gametophytic self-incompatibility in orchids suggests this system evolved after the monocot-eudicot split".

This study is part of submission 21138 (Status: Published).

Citation

Niu S.C., Luo Y., & Liu Z. 2017. Lack of S-RNase-based gametophytic self-incompatibility in orchids suggests this system evolved after the monocot-eudicot split. Frontiers in Plant Science, .

Authors

• Niu S.C.
• Luo Y.
• Liu Z.

Abstract

Self-incompatibility (SI) is found in approximately 40% of flowering plant species and at least 100 families. Although orchids belong to the largest angiosperm family, only 10% of the orchid species present SI and have gametophytic SI (GSI). Furthermore, a majority (72%) of Dendrobium species, which constitute one of the largest Orchidaceae genera, show SI and also have GSI. However, nothing is known about the molecular mechanism of GSI. The molecular characterization of the S-determinants of GSI is at an advanced state in Solanaceae, Rosaceae, and Plantaginaceae, which use an S-ribonuclease (S-RNase)-based system. Here, we investigate the hypothesis that Orchidaceae uses a similar S-RNase to the ones described in Rosaceae, Solanaceae, and Plantaginaceae SI species. In this study, two SI species (Dendrobium longicornu and D. chrysanthum) were identified using fluorescence microscopy. Then, the S-RNase- and SLF-interacting SKP1-like1 (SSK1)-like genes present in their transcriptomes and the genomes of Phalaenopsis equestris, D. catenatum, Vanilla shenzhenica, and Apostasia shenzhenica were investigated. Sequence, phylogenetic, and tissue-specific expression analyses revealed that none of the genes identified was an S-determinant, suggesting that Orchidaceae might have a novel SI mechanism. The results also suggested that RNase-based GSI might have evolved after the split of monocotyledons (monocots) and dicotyledons (dicots) but before the split of Asteridae and Rosidae. This is also the first study to investigate S-RNase-based GSI in monocots. However, studies on gene identification, differential expression, and segregation analyses in controlled crosses are needed to further evaluate the genes with high expression levels in GSI tissues.

Keywords

Orchidaceae, self-incompatibility, evolution, Transcription, Genetic, S-RNase based GSI

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S21138
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref27299,
  	 author = {Shan Ce Niu and Yi-Bo  Luo and Zhong-Jian  Liu},
  	 title = {Lack of S-RNase-based gametophytic self-incompatibility in orchids suggests this system evolved after the monocot-eudicot split},
  	 year = {2017},
  	 keywords = {Orchidaceae, self-incompatibility, evolution, Transcription, Genetic, S-RNase based GSI},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Frontiers in Plant Science},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Self-incompatibility (SI) is found in approximately 40% of flowering plant species and at least 100 families. Although orchids belong to the largest angiosperm family, only 10% of the orchid species present SI and have gametophytic SI (GSI). Furthermore, a majority (72%) of Dendrobium species, which constitute one of the largest Orchidaceae genera, show SI and also have GSI. However, nothing is known about the molecular mechanism of GSI. The molecular characterization of the S-determinants of GSI is at an advanced state in Solanaceae, Rosaceae, and Plantaginaceae, which use an S-ribonuclease (S-RNase)-based system. Here, we investigate the hypothesis that Orchidaceae uses a similar S-RNase to the ones described in Rosaceae, Solanaceae, and Plantaginaceae SI species. In this study, two SI species (Dendrobium longicornu and D. chrysanthum) were identified using fluorescence microscopy. Then, the S-RNase- and SLF-interacting SKP1-like1 (SSK1)-like genes present in their transcriptomes and the genomes of Phalaenopsis equestris, D. catenatum, Vanilla shenzhenica, and Apostasia shenzhenica were investigated. Sequence, phylogenetic, and tissue-specific expression analyses revealed that none of the genes identified was an S-determinant, suggesting that Orchidaceae might have a novel SI mechanism. The results also suggested that RNase-based GSI might have evolved after the split of monocotyledons (monocots) and dicotyledons (dicots) but before the split of Asteridae and Rosidae. This is also the first study to investigate S-RNase-based GSI in monocots. However, studies on gene identification, differential expression, and segregation analyses in controlled crosses are needed to further evaluate the genes with high expression levels in GSI tissues.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 27299
  AU  - Niu,Shan Ce
  AU  - Luo,Yi-Bo 
  AU  - Liu,Zhong-Jian 
  T1  - Lack of S-RNase-based gametophytic self-incompatibility in orchids suggests this system evolved after the monocot-eudicot split
  PY  - 2017
  KW  - Orchidaceae
  KW  - self-incompatibility
  KW  - evolution
  KW  - Transcription
  KW  - Genetic
  KW  - S-RNase based GSI
  UR  - http://dx.doi.org/
  N2  - Self-incompatibility (SI) is found in approximately 40% of flowering plant species and at least 100 families. Although orchids belong to the largest angiosperm family, only 10% of the orchid species present SI and have gametophytic SI (GSI). Furthermore, a majority (72%) of Dendrobium species, which constitute one of the largest Orchidaceae genera, show SI and also have GSI. However, nothing is known about the molecular mechanism of GSI. The molecular characterization of the S-determinants of GSI is at an advanced state in Solanaceae, Rosaceae, and Plantaginaceae, which use an S-ribonuclease (S-RNase)-based system. Here, we investigate the hypothesis that Orchidaceae uses a similar S-RNase to the ones described in Rosaceae, Solanaceae, and Plantaginaceae SI species. In this study, two SI species (Dendrobium longicornu and D. chrysanthum) were identified using fluorescence microscopy. Then, the S-RNase- and SLF-interacting SKP1-like1 (SSK1)-like genes present in their transcriptomes and the genomes of Phalaenopsis equestris, D. catenatum, Vanilla shenzhenica, and Apostasia shenzhenica were investigated. Sequence, phylogenetic, and tissue-specific expression analyses revealed that none of the genes identified was an S-determinant, suggesting that Orchidaceae might have a novel SI mechanism. The results also suggested that RNase-based GSI might have evolved after the split of monocotyledons (monocots) and dicotyledons (dicots) but before the split of Asteridae and Rosidae. This is also the first study to investigate S-RNase-based GSI in monocots. However, studies on gene identification, differential expression, and segregation analyses in controlled crosses are needed to further evaluate the genes with high expression levels in GSI tissues.
  L3  - 
  JF  - Frontiers in Plant Science
  VL  - 
  IS  - 
  ER  -