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Citation for Study 21620

Citation title: "Racocetra crispa (Glomeromycotina) delimited by integrative evidence based on morphology, long continuous nuclear rDNA sequencing and phylogeny".

Study name: "Racocetra crispa (Glomeromycotina) delimited by integrative evidence based on morphology, long continuous nuclear rDNA sequencing and phylogeny".

This study is part of submission 21620 (Status: Published).

Citation

De souza F., Da silva I.R., Barreto M.B., Oehl F., Goto B.T., & Maia L.C. 2018. Racocetra crispa (Glomeromycotina) delimited by integrative evidence based on morphology, long continuous nuclear rDNA sequencing and phylogeny. Mycological Progress, .

Authors

• De souza F.
• Da silva I.R.
• Barreto M.B.
• Oehl F.
• Goto B.T.
• Maia L.C.

Abstract

Here, we describe a new ornamented arbuscular mycorrhizal (AM) fungus, Racocetra crispa sp. nov. isolated from maize fields from the central region of Minas Gerais State, Brazil. For the first time, a Glomeromycotina species is described using a long continuous nuclear rDNA sequence fragment which encompasses the nearly complete 18S SSU sequence gene until the 3' end of the D2 region of the 28S LSU (~3100 bp) which allows for comparison with sequences obtained from regions used for fungal metagenomic studies, species description, and AM fungi DNA-barcode. The new species forms dark brown to black spores, approx. 340-510 μm on in diam., on sporogenous cells. The spores have unique cloud/flower' projections on the spore surface, two walls, and differentiate a multiple-lobed germ shield with up to 8-12 germ tube initiations. The analysis of the intra- and interspecific DNA-barcode sequence variation within the Racocetra showed that the intragenomic polymorphism among the clones of R. crispa (0-2%) is within the lower range for the genus. The V3-V4 region of the SSU nrDNA has no resolution to discriminate Racocetra at species level, but from this fragment we found homology between R. crispa and environmental sequences from two metagenomics studies, one carried out in Brazil at the fungus type location and the other in New Zealand. The integration between AM fungal sequences from reference strains and those obtained from environmental sequences in Glomeromycotina is still a problematic issue, mainly due to the reduced number of AM fungal species characterized based on DNA sequences.

Keywords

Glomeromycetes; DNA-Barcode; Gigasporales; Racocetraceae; integrative taxonomy; Cerrado; SNP, New Species

External links

• DOI: 10.1007/s11557-018-1410-9
• Other URL: http://link.springer.com/article/10.1007/s11557-018-1410-9

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S21620
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref27697,
  	 author = {Francisco  de Souza and Iolanda Ramalho  da Silva and Maria Beatriz Barbosa de Barros Barreto and Fritz  Oehl and Bruno  Tomio Goto and Leonor Costa Maia},
  	 title = {Racocetra crispa (Glomeromycotina) delimited by integrative evidence based on morphology, long continuous nuclear rDNA sequencing and phylogeny},
  	 year = {2018},
  	 keywords = {Glomeromycetes; DNA-Barcode; Gigasporales; Racocetraceae; integrative taxonomy; Cerrado; SNP, New Species },
  	 doi = {10.1007/s11557-018-1410-9},
  	 url = {http://link.springer.com/article/10.1007/s11557-018-1410-9},
  	 pmid = {},
  	 journal = {Mycological Progress},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Here, we describe a new ornamented arbuscular mycorrhizal (AM) fungus, Racocetra crispa sp. nov. isolated from maize fields from the central region of Minas Gerais State, Brazil. For the first time, a Glomeromycotina species is described using a long
  continuous nuclear rDNA sequence fragment which encompasses the nearly complete 18S SSU sequence gene until the 3' end of the D2 region of the 28S LSU (~3100 bp) which allows for comparison with sequences obtained from regions used for fungal
  metagenomic studies, species description, and AM fungi DNA-barcode. The new species forms dark brown to black spores, approx. 340-510 μm on in diam., on
  sporogenous cells. The spores have unique cloud/flower' projections on the spore
  surface, two walls, and differentiate a multiple-lobed germ shield with up to 8-12 germ tube initiations. The analysis of the intra- and interspecific DNA-barcode sequence variation within the Racocetra showed that the intragenomic polymorphism among the clones of R. crispa (0-2%) is within the lower range for the genus. The V3-V4 region of the SSU nrDNA has no resolution to discriminate Racocetra at species level, but from
  this fragment we found homology between R. crispa and environmental sequences from two metagenomics studies, one carried out in Brazil at the fungus type location and the other in New Zealand. The integration between AM fungal sequences from
  reference strains and those obtained from environmental sequences in Glomeromycotina is still a problematic issue, mainly due to the reduced number of AM fungal species characterized based on DNA sequences.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 27697
  AU  - de Souza,Francisco 
  AU  - da Silva,Iolanda Ramalho 
  AU  - Barreto,Maria Beatriz Barbosa de Barros
  AU  - Oehl,Fritz 
  AU  - Goto,Bruno  Tomio
  AU  - Maia,Leonor Costa
  T1  - Racocetra crispa (Glomeromycotina) delimited by integrative evidence based on morphology, long continuous nuclear rDNA sequencing and phylogeny
  PY  - 2018
  KW  - Glomeromycetes; DNA-Barcode; Gigasporales; Racocetraceae; integrative taxonomy; Cerrado; SNP
  KW  - New Species 
  UR  - http://link.springer.com/article/10.1007/s11557-018-1410-9
  N2  - Here, we describe a new ornamented arbuscular mycorrhizal (AM) fungus, Racocetra crispa sp. nov. isolated from maize fields from the central region of Minas Gerais State, Brazil. For the first time, a Glomeromycotina species is described using a long
  continuous nuclear rDNA sequence fragment which encompasses the nearly complete 18S SSU sequence gene until the 3' end of the D2 region of the 28S LSU (~3100 bp) which allows for comparison with sequences obtained from regions used for fungal
  metagenomic studies, species description, and AM fungi DNA-barcode. The new species forms dark brown to black spores, approx. 340-510 μm on in diam., on
  sporogenous cells. The spores have unique cloud/flower' projections on the spore
  surface, two walls, and differentiate a multiple-lobed germ shield with up to 8-12 germ tube initiations. The analysis of the intra- and interspecific DNA-barcode sequence variation within the Racocetra showed that the intragenomic polymorphism among the clones of R. crispa (0-2%) is within the lower range for the genus. The V3-V4 region of the SSU nrDNA has no resolution to discriminate Racocetra at species level, but from
  this fragment we found homology between R. crispa and environmental sequences from two metagenomics studies, one carried out in Brazil at the fungus type location and the other in New Zealand. The integration between AM fungal sequences from
  reference strains and those obtained from environmental sequences in Glomeromycotina is still a problematic issue, mainly due to the reduced number of AM fungal species characterized based on DNA sequences.
  L3  - 10.1007/s11557-018-1410-9
  JF  - Mycological Progress
  VL  - 
  IS  - 
  ER  -