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Citation for Study 21713

Citation title: "Integrative analyses of Nervilia (Orchidaceae) section Linervia reveal further undescribed cryptic diversity in Thailand".

Study name: "Integrative analyses of Nervilia (Orchidaceae) section Linervia reveal further undescribed cryptic diversity in Thailand".

This study is part of submission 21713 (Status: Published).

Citation

Gale S.W., Duangjai S., Li J., Ito Y., Watthana S., Termwutthipreecha P., & Suddee S. 2017. Integrative analyses of Nervilia (Orchidaceae) section Linervia reveal further undescribed cryptic diversity in Thailand. Systematics and Biodiversity, .

Authors

• Gale S.W.
• Duangjai S.
• Li J. (submitter) 00852-24837142
• Ito Y.
• Watthana S.
• Termwutthipreecha P.
• Suddee S.

Abstract

The delimitation of cryptic species is necessary to accurately classify and appropriately conserve biodiversity. Integrative analyses can be incisive in detecting and circumscribing cryptic diversity, especially in species complexes whose members are delineated by minor or overlapping morphological variation. We adopt an integrative approach to assess species relationships and resolve species boundaries in the taxonomically difficult Nervilia adolphi/punctata species alliance of N. sect. Linervia, an Old World complex of reduced, one-flowered terrestrial orchids that is both species-rich and poorly known in tropical and warm temperate Asia. We sampled 12 of the 27 known species of the alliance in Asia, including all four species reported from Thailand and a further 20 plants collected in that country that could not be satisfactorily identified using morphology alone. Phylogenetic analyses using one nuclear (ITS) and two plastid (matK and trnL-F) markers confirmed both N. sect. Linervia and the alliance itself as monophyletic, and corroborated 11 of the 12 sampled species; N. punctata proved polyphyletic, with the Thai samples referred to this Indonesian species falling sister to the Himalayan N. mackinnonii. The 20 unidentified Thai samples formed three distinct, strongly supported clades. STACEY, a Bayesian coalescence approach to species delimitation, resolved the same three clusters, but provided evidence suggesting that one comprised two distinct sub-clades. Building on this genetic evidence, we identify subtle morphological differences and invoke a diagnosable species concept to circumscribe three previously unrecognised cryptic species from Thailand. This objective approach to species delimitation validates ostensibly minor morphological differences as a basis for differentiating species within the alliance, and paves the way for a global analysis of species boundaries throughout the genus as a whole.

Keywords

cryptic species; integrative analyses; morphology; Nervilia adolphi/punctata species alliance; phylogenetic analysis; section Linervia; species complex; species delimitation; STACEY.

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S21713
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref27727,
  	 author = {Stephan W. Gale and Sutee  Duangjai and Jihong  Li and Yu  Ito and Santi   Watthana and Phatsara   Termwutthipreecha and Somran  Suddee},
  	 title = {Integrative analyses of Nervilia (Orchidaceae) section Linervia reveal further undescribed cryptic diversity in Thailand},
  	 year = {2017},
  	 keywords = {cryptic species; integrative analyses; morphology; Nervilia adolphi/punctata species alliance; phylogenetic analysis; section Linervia; species complex; species delimitation; STACEY.},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Systematics and Biodiversity},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {The delimitation of cryptic species is necessary to accurately classify and appropriately conserve biodiversity. Integrative analyses can be incisive in detecting and circumscribing cryptic diversity, especially in species complexes whose members are delineated by minor or overlapping morphological variation. We adopt an integrative approach to assess species relationships and resolve species boundaries in the taxonomically difficult Nervilia adolphi/punctata species alliance of N. sect. Linervia, an Old World complex of reduced, one-flowered terrestrial orchids that is both species-rich and poorly known in tropical and warm temperate Asia. We sampled 12 of the 27 known species of the alliance in Asia, including all four species reported from Thailand and a further 20 plants collected in that country that could not be satisfactorily identified using morphology alone. Phylogenetic analyses using one nuclear (ITS) and two plastid (matK and trnL-F) markers confirmed both N. sect. Linervia and the alliance itself as monophyletic, and corroborated 11 of the 12 sampled species; N. punctata proved polyphyletic, with the Thai samples referred to this Indonesian species falling sister to the Himalayan N. mackinnonii. The 20 unidentified Thai samples formed three distinct, strongly supported clades. STACEY, a Bayesian coalescence approach to species delimitation, resolved the same three clusters, but provided evidence suggesting that one comprised two distinct sub-clades. Building on this genetic evidence, we identify subtle morphological differences and invoke a diagnosable species concept to circumscribe three previously unrecognised cryptic species from Thailand. This objective approach to species delimitation validates ostensibly minor morphological differences as a basis for differentiating species within the alliance, and paves the way for a global analysis of species boundaries throughout the genus as a whole.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 27727
  AU  - Gale,Stephan W.
  AU  - Duangjai,Sutee 
  AU  - Li,Jihong 
  AU  - Ito,Yu 
  AU  - Watthana,Santi  
  AU  - Termwutthipreecha,Phatsara  
  AU  - Suddee,Somran 
  T1  - Integrative analyses of Nervilia (Orchidaceae) section Linervia reveal further undescribed cryptic diversity in Thailand
  PY  - 2017
  KW  - cryptic species; integrative analyses; morphology; Nervilia adolphi/punctata species alliance; phylogenetic analysis; section Linervia; species complex; species delimitation; STACEY.
  UR  - http://dx.doi.org/
  N2  - The delimitation of cryptic species is necessary to accurately classify and appropriately conserve biodiversity. Integrative analyses can be incisive in detecting and circumscribing cryptic diversity, especially in species complexes whose members are delineated by minor or overlapping morphological variation. We adopt an integrative approach to assess species relationships and resolve species boundaries in the taxonomically difficult Nervilia adolphi/punctata species alliance of N. sect. Linervia, an Old World complex of reduced, one-flowered terrestrial orchids that is both species-rich and poorly known in tropical and warm temperate Asia. We sampled 12 of the 27 known species of the alliance in Asia, including all four species reported from Thailand and a further 20 plants collected in that country that could not be satisfactorily identified using morphology alone. Phylogenetic analyses using one nuclear (ITS) and two plastid (matK and trnL-F) markers confirmed both N. sect. Linervia and the alliance itself as monophyletic, and corroborated 11 of the 12 sampled species; N. punctata proved polyphyletic, with the Thai samples referred to this Indonesian species falling sister to the Himalayan N. mackinnonii. The 20 unidentified Thai samples formed three distinct, strongly supported clades. STACEY, a Bayesian coalescence approach to species delimitation, resolved the same three clusters, but provided evidence suggesting that one comprised two distinct sub-clades. Building on this genetic evidence, we identify subtle morphological differences and invoke a diagnosable species concept to circumscribe three previously unrecognised cryptic species from Thailand. This objective approach to species delimitation validates ostensibly minor morphological differences as a basis for differentiating species within the alliance, and paves the way for a global analysis of species boundaries throughout the genus as a whole.
  L3  - 
  JF  - Systematics and Biodiversity
  VL  - 
  IS  - 
  ER  -