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Citation for Study 22357

Citation title: "Geographic distribution and molecular analysis of porcine reproductive and respiratory syndrome viruses circulating in swine farms in the Republic of Korea between 2013 and 2016".

Study name: "Geographic distribution and molecular analysis of porcine reproductive and respiratory syndrome viruses circulating in swine farms in the Republic of Korea between 2013 and 2016".

This study is part of submission 22357 (Status: Published).

Citation

Kang H., Yu J., Shin J., Kang A., Kim W., Lee C., Lee J., Cho I., Choe S., & Cha S. 2018. Geographic distribution and molecular analysis of porcine reproductive and respiratory syndrome viruses circulating in swine farms in the Republic of Korea between 2013 and 2016. BMC Veterinary Research, .

Authors

• Kang H. (submitter) +821094929616
• Yu J.
• Shin J.
• Kang A.
• Kim W.
• Lee C.
• Lee J.
• Cho I.
• Choe S.
• Cha S.

Abstract

Background:Porcine reproductive and respiratory syndrome virus (PRRSV) causes devastating disease characterized by reproductive failure and respiratory problems in the swine industry. To understand the recent prevalence and genetic diversity of field PRRSVs in the Republic of Korea, open reading frames (ORFs) 5 and 7 of PRRSV field isolates frome 631 PRRS-affected-swine farms nationwide in 2013-2016 were analyzed along with 200 Korean field viruses isolated in 2003-2010, and 113 foreign field and vaccine strains. Result: Korean swine farms were widely infected with PRRSVs of a single type (38.4% and 37.4% for Type 1 and Type 2 PRRSV, respectively) or both types (24.2%) with up to approximately 83% nucleotide sequence similarity to prototype PRRSVs (Lelystad or VR2332). Phylogenetic analysis based on the ORF5 nucleotide sequence revealed that Korean Type 1 field isolates were classified as subgroups A, B and C under subtype 1, while Korean Type 2 field isolates were classified as lineage 1 and 5 as well as three Korean lineages (kor A, B and C) with the highest infection prevalence in subgroup A (50.5%) and lineage 5 (15.3%) for Type 1 and Type 2 PRRSV, respectively, among ORF5-positive farms. In particular, the lineages kor B and C were identified as novel lineages in this study, and lineage kor B comprised only the field viruses isolated from Gyeongnam Province in 2014-2015, establishing regionally unique genetic characteristics. It has also recently been confirmed that commercialized vaccine-like viruses (subgroup C) of Type 1 PRRSV and NADC30-like viruses of Type 2 PRRSV (lineage 1) are spreading rapidly in Korean swine farms. The Korean field viruses were also expected to be antigenically variable as shown in the high diversity of neutralizing epitopes and N-glycosylation sites. conclusions : This up-to-date information regarding recent field PRRSVs should be taken into consideration when creating strategies for the application of PRRS control measures, including vaccination in the field.

Keywords

porcine reproductive and respiratory syndrome virus (PRRSV), Genetic diversity, open reading frame 5 (ORF5) sequence, Phylogenetic analysis

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S22357
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref28192,
  	 author = {Hyeonjeong  Kang and Ji Eun  Yu and Ji-Eun  Shin and Areum  Kang and Won-Il  Kim and Changhee  Lee and Jienny  Lee and In-Soo  Cho and Se-Eun  Choe and Sang-Ho   Cha},
  	 title = {Geographic distribution and molecular analysis of porcine reproductive and respiratory syndrome viruses circulating in swine farms in the Republic of Korea between 2013 and 2016},
  	 year = {2018},
  	 keywords = {porcine reproductive and respiratory syndrome virus (PRRSV), Genetic diversity, open reading frame 5 (ORF5) sequence, Phylogenetic analysis},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {BMC Veterinary Research},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Background:Porcine reproductive and respiratory syndrome virus (PRRSV) causes devastating disease characterized by reproductive failure and respiratory problems in the swine industry. To understand the recent prevalence and genetic diversity of field PRRSVs in the Republic of Korea, open reading frames (ORFs) 5 and 7 of PRRSV field isolates frome 631 PRRS-affected-swine farms nationwide in 2013-2016 were analyzed along with 200 Korean field viruses isolated in 2003-2010, and 113 foreign field and vaccine strains.
  Result: Korean swine farms were widely infected with PRRSVs of a single type (38.4% and 37.4% for Type 1 and Type 2 PRRSV, respectively) or both types (24.2%) with up to approximately 83% nucleotide sequence similarity to prototype PRRSVs (Lelystad or VR2332). Phylogenetic analysis based on the ORF5 nucleotide sequence revealed that Korean Type 1 field isolates were classified as subgroups A, B and C under subtype 1, while Korean Type 2 field isolates were classified as lineage 1 and 5 as well as three Korean lineages (kor A, B and C) with the highest infection prevalence in subgroup A (50.5%) and lineage 5 (15.3%) for Type 1 and Type 2 PRRSV, respectively, among ORF5-positive farms. In particular, the lineages kor B and C were identified as novel lineages in this study, and lineage kor B comprised only the field viruses isolated from Gyeongnam Province in 2014-2015, establishing regionally unique genetic characteristics. It has also recently been confirmed that commercialized vaccine-like viruses (subgroup C) of Type 1 PRRSV and NADC30-like viruses of Type 2 PRRSV (lineage 1) are spreading rapidly in Korean swine farms. The Korean field viruses were also expected to be antigenically variable as shown in the high diversity of neutralizing epitopes and N-glycosylation sites.
  conclusions : This up-to-date information regarding recent field PRRSVs should be taken into consideration when creating strategies for the application of PRRS control measures, including vaccination in the field.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 28192
  AU  - Kang,Hyeonjeong 
  AU  - Yu,Ji Eun 
  AU  - Shin,Ji-Eun 
  AU  - Kang,Areum 
  AU  - Kim,Won-Il 
  AU  - Lee,Changhee 
  AU  - Lee,Jienny 
  AU  - Cho,In-Soo 
  AU  - Choe,Se-Eun 
  AU  - Cha,Sang-Ho  
  T1  - Geographic distribution and molecular analysis of porcine reproductive and respiratory syndrome viruses circulating in swine farms in the Republic of Korea between 2013 and 2016
  PY  - 2018
  KW  - porcine reproductive and respiratory syndrome virus (PRRSV)
  KW  - Genetic diversity
  KW  - open reading frame 5 (ORF5) sequence
  KW  - Phylogenetic analysis
  UR  - http://dx.doi.org/
  N2  - Background:Porcine reproductive and respiratory syndrome virus (PRRSV) causes devastating disease characterized by reproductive failure and respiratory problems in the swine industry. To understand the recent prevalence and genetic diversity of field PRRSVs in the Republic of Korea, open reading frames (ORFs) 5 and 7 of PRRSV field isolates frome 631 PRRS-affected-swine farms nationwide in 2013-2016 were analyzed along with 200 Korean field viruses isolated in 2003-2010, and 113 foreign field and vaccine strains.
  Result: Korean swine farms were widely infected with PRRSVs of a single type (38.4% and 37.4% for Type 1 and Type 2 PRRSV, respectively) or both types (24.2%) with up to approximately 83% nucleotide sequence similarity to prototype PRRSVs (Lelystad or VR2332). Phylogenetic analysis based on the ORF5 nucleotide sequence revealed that Korean Type 1 field isolates were classified as subgroups A, B and C under subtype 1, while Korean Type 2 field isolates were classified as lineage 1 and 5 as well as three Korean lineages (kor A, B and C) with the highest infection prevalence in subgroup A (50.5%) and lineage 5 (15.3%) for Type 1 and Type 2 PRRSV, respectively, among ORF5-positive farms. In particular, the lineages kor B and C were identified as novel lineages in this study, and lineage kor B comprised only the field viruses isolated from Gyeongnam Province in 2014-2015, establishing regionally unique genetic characteristics. It has also recently been confirmed that commercialized vaccine-like viruses (subgroup C) of Type 1 PRRSV and NADC30-like viruses of Type 2 PRRSV (lineage 1) are spreading rapidly in Korean swine farms. The Korean field viruses were also expected to be antigenically variable as shown in the high diversity of neutralizing epitopes and N-glycosylation sites.
  conclusions : This up-to-date information regarding recent field PRRSVs should be taken into consideration when creating strategies for the application of PRRS control measures, including vaccination in the field.
  L3  - 
  JF  - BMC Veterinary Research
  VL  - 
  IS  - 
  ER  -