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Citation for Study 23290

Citation title: "Green Fluorescent Protein Transformation Sheds More Light on a Widespread Mycoparasitic Interaction".

Study name: "Green Fluorescent Protein Transformation Sheds More Light on a Widespread Mycoparasitic Interaction".

This study is part of submission 23290 (Status: Published).

Citation

Nemeth M.Z., Pintye A., Horv?th ?.N., V?gi P., Kov?cs G., Gorfer M., & Kiss L. 2019. Green Fluorescent Protein Transformation Sheds More Light on a Widespread Mycoparasitic Interaction. Phytopathology, 109(8): 1404-1416.

Authors

• Nemeth M.Z. (submitter) 00614877549
• Pintye A.
• Horv?th ?.N.
• V?gi P.
• Kov?cs G.
• Gorfer M.
• Kiss L.

Abstract

Powdery mildews, ubiquitous obligate biotrophic plant pathogens, are often attacked in the field by mycoparasitic fungi belonging to the genus Ampelomyces. Some Ampelomyces strains are commercialized biocontrol agents of crop pathogenic powdery mildews. Using Agrobacterium tumefaciens-mediated transformation (ATMT), we produced stable Ampelomyces transformants that constitutively expressed green fluorescent protein (GFP) to (i) improve the visualization of the mildew? Ampelomyces interaction and (ii) decipher the environmental fate of Ampelomyces fungi before and after acting as a mycoparasite. Detection of Ampelomyces structures, and especially hyphae, was greatly enhanced when diverse powdery mildew, leaf, and soil samples containing GFP transformants were examined with fluorescence microscopy compared with brightfield and differential interference contrast optics. We showed for the first time, to our knowledge, that Ampelomyces strains can persist up to 21 days on mildew-free host plant surfaces, where they can attack powdery mildew structures as soon as these appear after this period. As saprobes in decomposing, powdery mildew-infected leaves on the ground and also in autoclaved soil, Ampelomyces strains developed new hyphae but did not sporulate. These results indicate that Ampelomyces strains occupy a niche in the phyllosphere where they act primarily as mycoparasites of powdery mildews. Our work has established a framework for a molecular genetic toolbox for the genus Ampelomyces using ATMT.

Keywords

tritrophic interactions

External links

• DOI: 10.1094/PHYTO-01-19-0013-R
• PMID: 30900938
• Other URL: http://apsjournals.apsnet.org/doi/10.1094/PHYTO-01-19-0013-R

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S23290
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref29468,
  	 author = {Mark Z. Nemeth and Alexandra  Pintye and ?ron N. Horv?th and P?l  V?gi and G?bor M.  Kov?cs and Markus  Gorfer and Levente  Kiss},
  	 title = {Green Fluorescent Protein Transformation Sheds More Light on a Widespread Mycoparasitic Interaction},
  	 year = {2019},
  	 keywords = {tritrophic interactions},
  	 doi = {10.1094/PHYTO-01-19-0013-R},
  	 url = {http://apsjournals.apsnet.org/doi/10.1094/PHYTO-01-19-0013-R},
  	 pmid = {30900938},
  	 journal = {Phytopathology},
  	 volume = {109},
  	 number = {8},
  	 pages = {1404--1416},
  	 abstract = {Powdery mildews, ubiquitous obligate biotrophic plant pathogens, are often attacked in the field by mycoparasitic fungi belonging to the genus Ampelomyces. Some Ampelomyces strains are commercialized biocontrol agents of crop pathogenic powdery mildews. Using Agrobacterium tumefaciens-mediated transformation (ATMT), we produced stable Ampelomyces transformants that constitutively expressed green fluorescent protein (GFP) to (i) improve the visualization of the mildew? Ampelomyces interaction and (ii) decipher the environmental fate of Ampelomyces fungi before and after acting as a mycoparasite. Detection of Ampelomyces structures, and especially hyphae, was greatly enhanced when diverse powdery mildew, leaf, and soil samples containing GFP transformants were examined with fluorescence microscopy compared with brightfield and differential interference contrast optics. We showed for the first time, to our knowledge, that Ampelomyces strains can persist up to 21 days on mildew-free host plant surfaces, where they can attack powdery mildew structures as soon as these appear after this period. As saprobes in decomposing, powdery mildew-infected leaves on the ground and also in autoclaved soil, Ampelomyces strains developed new hyphae but did not sporulate. These results indicate that Ampelomyces strains occupy a niche in the phyllosphere where they act primarily as mycoparasites of powdery mildews. Our work has established a framework for a molecular genetic toolbox for the genus Ampelomyces using ATMT.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 29468
  AU  - Nemeth,Mark Z.
  AU  - Pintye,Alexandra 
  AU  - Horv?th,?ron N.
  AU  - V?gi,P?l 
  AU  - Kov?cs,G?bor M. 
  AU  - Gorfer,Markus 
  AU  - Kiss,Levente 
  T1  - Green Fluorescent Protein Transformation Sheds More Light on a Widespread Mycoparasitic Interaction
  PY  - 2019
  KW  - tritrophic interactions
  UR  - http://apsjournals.apsnet.org/doi/10.1094/PHYTO-01-19-0013-R
  N2  - Powdery mildews, ubiquitous obligate biotrophic plant pathogens, are often attacked in the field by mycoparasitic fungi belonging to the genus Ampelomyces. Some Ampelomyces strains are commercialized biocontrol agents of crop pathogenic powdery mildews. Using Agrobacterium tumefaciens-mediated transformation (ATMT), we produced stable Ampelomyces transformants that constitutively expressed green fluorescent protein (GFP) to (i) improve the visualization of the mildew? Ampelomyces interaction and (ii) decipher the environmental fate of Ampelomyces fungi before and after acting as a mycoparasite. Detection of Ampelomyces structures, and especially hyphae, was greatly enhanced when diverse powdery mildew, leaf, and soil samples containing GFP transformants were examined with fluorescence microscopy compared with brightfield and differential interference contrast optics. We showed for the first time, to our knowledge, that Ampelomyces strains can persist up to 21 days on mildew-free host plant surfaces, where they can attack powdery mildew structures as soon as these appear after this period. As saprobes in decomposing, powdery mildew-infected leaves on the ground and also in autoclaved soil, Ampelomyces strains developed new hyphae but did not sporulate. These results indicate that Ampelomyces strains occupy a niche in the phyllosphere where they act primarily as mycoparasites of powdery mildews. Our work has established a framework for a molecular genetic toolbox for the genus Ampelomyces using ATMT.
  L3  - 10.1094/PHYTO-01-19-0013-R
  JF  - Phytopathology
  VL  - 109
  IS  - 8
  SP  - 1404
  EP  - 1416
  ER  -