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Citation for Study 25554

Citation title: "Differences in the Characteristics and Pathogenicity of Pyrenophora Species Associated with the Seeds of Italian Ryegrass".

Study name: "Differences in the Characteristics and Pathogenicity of Pyrenophora Species Associated with the Seeds of Italian Ryegrass".

This study is part of submission 25554 (Status: Published).

Citation

Xue L. 2023. Differences in the Characteristics and Pathogenicity of Pyrenophora Species Associated with the Seeds of Italian Ryegrass. Plant Disease, 107(3): 758-770.

Authors

• Xue L. (submitter) 17608247609

Abstract

Pyrenophora is a genus of pathogens that cause leaf damage and a common seedborne fungus of Italian ryegrass (Lolium multiflorum Lam.). To elucidate the main seedborne Pyrenophora species, 36 seed samples of Italian ryegrass were collected; in total, 113 strains, representing 12.3% of all isolated strains (921), were identified as Pyrenophora species using the identity of ITS sequences in NCBI and the similarity of morphological characteristics. P. dictyoides(97) was the most frequent species.By pure culture technique, 24 representative pure isolates were obtained for further study. Based on DNA analysis of multiple loci (ITS, LSU, GPDH,CHS-1, and RPB1) and morphological characters, eight Pyrenophora species were identified, P. avenicola, P. chaetomioides, P. dictyoides,P. lolii, P. nobleae, P. teres, P. triseptata, and P. tritici-repentis; among them, P. avenicola, P. tritici-repentis,and P. triseptata were newly reported on Italian ryegrass worldwide. Seed inoculation showed that P. dictyoides, P. lolii, and P. teres remarkably decreased the final germination percentages and germination indexes compared with control treatments (P # 0.05); and plant inoculation showed that P. dictyoides, P. lolii,and P. nobleae could cause typical brown spot in vivo with a higher infection rate (P # 0.05). In conclusion, pathogenicity tests showed that all Pyrenophora species could both inhibit seed germination and infect Italian ryegrass to different degrees; among them, P. dictyoides was the most important seedborne pathogen based on the combination of its isolation and infection rate, followed by P. lolii and P. nobleae. The data generated in this study are helpful for the accurate identification of Pyrenophora species and the development of seedborne disease management strategies.

Keywords

Lolium multiflorum, multiple loci, Pyrenophora species, seedborne

External links

• DOI: 10.1094/PDIS-08-22-1753-RE
• Other URL: http://doi.org/10.1094/PDIS-08-22-1753-RE

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S25554
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref30299,
  	 author = {Longhai  Xue},
  	 title = {Differences in the Characteristics and Pathogenicity of Pyrenophora Species Associated with the Seeds of Italian Ryegrass},
  	 year = {2023},
  	 keywords = {Lolium multiflorum, multiple loci, Pyrenophora species, seedborne},
  	 doi = {10.1094/PDIS-08-22-1753-RE},
  	 url = {http://doi.org/10.1094/PDIS-08-22-1753-RE},
  	 pmid = {},
  	 journal = {Plant Disease},
  	 volume = {107},
  	 number = {3},
  	 pages = {758--770},
  	 abstract = {Pyrenophora is a genus of pathogens that cause leaf damage and a common seedborne fungus of Italian ryegrass (Lolium multiflorum Lam.). To elucidate the main seedborne Pyrenophora species, 36 seed samples of Italian ryegrass were collected; in total, 113 strains, representing 12.3% of all isolated strains (921), were identified as Pyrenophora species using the identity of ITS sequences in NCBI and the similarity of morphological characteristics. P. dictyoides(97) was the most frequent species.By pure culture technique, 24 representative pure isolates were obtained for further study. Based on DNA analysis of multiple loci (ITS, LSU, GPDH,CHS-1, and RPB1) and morphological characters, eight Pyrenophora species were identified, P. avenicola, P. chaetomioides, P. dictyoides,P. lolii, P. nobleae, P. teres, P. triseptata, and P. tritici-repentis; among them, P. avenicola, P. tritici-repentis,and P. triseptata were newly reported on Italian ryegrass worldwide. Seed inoculation showed that P. dictyoides, P. lolii, and P. teres remarkably decreased the final germination percentages and germination indexes compared with control treatments (P # 0.05); and plant inoculation showed that P. dictyoides, P. lolii,and P. nobleae could cause typical brown spot in vivo with a higher infection rate (P # 0.05). In conclusion, pathogenicity tests showed that all Pyrenophora species could both inhibit seed germination and infect Italian ryegrass to different degrees; among them, P. dictyoides was the most important seedborne pathogen based on the combination of its isolation and infection rate, followed by P. lolii and P. nobleae. The data generated in this study are helpful for the accurate identification of Pyrenophora species and the development of seedborne disease management strategies.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 30299
  AU  - Xue,Longhai 
  T1  - Differences in the Characteristics and Pathogenicity of Pyrenophora Species Associated with the Seeds of Italian Ryegrass
  PY  - 2023
  KW  - Lolium multiflorum
  KW  - multiple loci
  KW  - Pyrenophora species
  KW  - seedborne
  UR  - http://doi.org/10.1094/PDIS-08-22-1753-RE
  N2  - Pyrenophora is a genus of pathogens that cause leaf damage and a common seedborne fungus of Italian ryegrass (Lolium multiflorum Lam.). To elucidate the main seedborne Pyrenophora species, 36 seed samples of Italian ryegrass were collected; in total, 113 strains, representing 12.3% of all isolated strains (921), were identified as Pyrenophora species using the identity of ITS sequences in NCBI and the similarity of morphological characteristics. P. dictyoides(97) was the most frequent species.By pure culture technique, 24 representative pure isolates were obtained for further study. Based on DNA analysis of multiple loci (ITS, LSU, GPDH,CHS-1, and RPB1) and morphological characters, eight Pyrenophora species were identified, P. avenicola, P. chaetomioides, P. dictyoides,P. lolii, P. nobleae, P. teres, P. triseptata, and P. tritici-repentis; among them, P. avenicola, P. tritici-repentis,and P. triseptata were newly reported on Italian ryegrass worldwide. Seed inoculation showed that P. dictyoides, P. lolii, and P. teres remarkably decreased the final germination percentages and germination indexes compared with control treatments (P # 0.05); and plant inoculation showed that P. dictyoides, P. lolii,and P. nobleae could cause typical brown spot in vivo with a higher infection rate (P # 0.05). In conclusion, pathogenicity tests showed that all Pyrenophora species could both inhibit seed germination and infect Italian ryegrass to different degrees; among them, P. dictyoides was the most important seedborne pathogen based on the combination of its isolation and infection rate, followed by P. lolii and P. nobleae. The data generated in this study are helpful for the accurate identification of Pyrenophora species and the development of seedborne disease management strategies.
  L3  - 10.1094/PDIS-08-22-1753-RE
  JF  - Plant Disease
  VL  - 107
  IS  - 3
  SP  - 758
  EP  - 770
  ER  -