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Citation for Study 25708

Citation title: "Fumonisin and Beauvericin Chemotypes and Genotypes of the Sister Species Fusarium subglutinans and Fusarium temperatum".

Study name: "Fumonisin and Beauvericin Chemotypes and Genotypes of the Sister Species Fusarium subglutinans and Fusarium temperatum".

This study is part of submission 25708 (Status: Published).

Citation

Fumero M.V., Villani A., Susca A., Haidukowski M., Cimmarusti M.T., Toomajian C., Leslie J., Chulze S., & Moretti A. 2020. Fumonisin and Beauvericin Chemotypes and Genotypes of the Sister Species Fusarium subglutinans and Fusarium temperatum. Applied and Environmental Microbiology, .

Authors

• Fumero M.V. (submitter) +54 9 358 5042363
• Villani A. +39 3401397112
• Susca A.
• Haidukowski M.
• Cimmarusti M.T.
• Toomajian C.
• Leslie J.
• Chulze S.
• Moretti A.

Abstract

Fusarium subglutinans and F. temperatum are common maize pathogens that produce mycotoxins and cause plant disease. The ability of these species to produce beauvericin and fumonisin mycotoxins is not settled, as reports of toxin production are not concordant. Our objective was to resolve the reported discrepancies by determining both chemotypes and genotypes for strains from both species. We analyzed 25 strains ? 13 of F. subglutinans and 12 of F. temperatum ? for toxin production by UPLC-MS. We used new genome sequences from two strains of F. subglutinans and one strain of F. temperatum, plus genomes of other Fusarium species, to determine the presence of functional gene clusters for the synthesis of these toxins. None of the strains of either species produced fumonisins. These strains also lack FUM biosynthetic genes, but retain homologs of some genes that flank the FUM cluster in F. verticillioides. None of the F. subglutinans strains produced beauvericin, although nine of 12 F. temperatum strains did. A complete beauvericin (BEA) gene cluster was present in all three new genome sequences. BEA1 was functional in F. temperatum, but was not functional in F. subglutinans due to a large insertion and multiple mutations that resulted in premature stop codons. The pattern of mutation accumulation for BEA1 suggests that its process of inactivation is relatively recent. Thus, neither F. subglutinans nor F. temperatum can produce fumonisins and F. subglutinans cannot produce beauvericin. Variation in the ability of strains of F. temperatum to produce beauvericin requires further study and could reflect the recent shared ancestry of these two species.

Keywords

Fusarium subglutinans, Fusarium temperatum, beauvericin, fumonisin, genomic cluster, toxigenic profiles

External links

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S25708
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref30412,
  	 author = {Maria Veronica Fumero and Alessandra  Villani and Antonia  Susca and Miriam  Haidukowski and M. T. Cimmarusti and Christopher  Toomajian and John F.  Leslie and Sofia  Chulze and Antonio  Moretti},
  	 title = {Fumonisin and Beauvericin Chemotypes and Genotypes of the Sister Species Fusarium subglutinans and Fusarium temperatum},
  	 year = {2020},
  	 keywords = {Fusarium subglutinans, Fusarium temperatum, beauvericin, fumonisin, genomic cluster, toxigenic profiles},
  	 doi = {},
  	 url = {http://},
  	 pmid = {},
  	 journal = {Applied and Environmental Microbiology},
  	 volume = {},
  	 number = {},
  	 pages = {},
  	 abstract = {Fusarium subglutinans and F. temperatum are common maize pathogens that produce mycotoxins and cause plant disease. The ability of these species to produce beauvericin and fumonisin mycotoxins is not settled, as reports of toxin production are not concordant. Our objective was to resolve the reported discrepancies by determining both chemotypes and genotypes for strains from both species. We analyzed 25 strains ? 13 of F. subglutinans and 12 of F. temperatum ? for toxin production by UPLC-MS. We used new genome sequences from two strains of F. subglutinans and one strain of F. temperatum, plus genomes of other Fusarium species, to determine the presence of functional gene clusters for the synthesis of these toxins. None of the strains of either species produced fumonisins. These strains also lack FUM biosynthetic genes, but retain homologs of some genes that flank the FUM cluster in F. verticillioides. None of the F. subglutinans strains produced beauvericin, although nine of 12 F. temperatum strains did. A complete beauvericin (BEA) gene cluster was present in all three new genome sequences. BEA1 was functional in F. temperatum, but was not functional in F. subglutinans due to a large insertion and multiple mutations that resulted in premature stop codons. The pattern of mutation accumulation for BEA1 suggests that its process of inactivation is relatively recent. Thus, neither F. subglutinans nor F. temperatum can produce fumonisins and F. subglutinans cannot produce beauvericin. Variation in the ability of strains of F. temperatum to produce beauvericin requires further study and could reflect the recent shared ancestry of these two species.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 30412
  AU  - Fumero,Maria Veronica
  AU  - Villani,Alessandra 
  AU  - Susca,Antonia 
  AU  - Haidukowski,Miriam 
  AU  - Cimmarusti,M. T.
  AU  - Toomajian,Christopher 
  AU  - Leslie,John F. 
  AU  - Chulze,Sofia 
  AU  - Moretti,Antonio 
  T1  - Fumonisin and Beauvericin Chemotypes and Genotypes of the Sister Species Fusarium subglutinans and Fusarium temperatum
  PY  - 2020
  KW  - Fusarium subglutinans
  KW  - Fusarium temperatum
  KW  - beauvericin
  KW  - fumonisin
  KW  - genomic cluster
  KW  - toxigenic profiles
  UR  - http://dx.doi.org/
  N2  - Fusarium subglutinans and F. temperatum are common maize pathogens that produce mycotoxins and cause plant disease. The ability of these species to produce beauvericin and fumonisin mycotoxins is not settled, as reports of toxin production are not concordant. Our objective was to resolve the reported discrepancies by determining both chemotypes and genotypes for strains from both species. We analyzed 25 strains ? 13 of F. subglutinans and 12 of F. temperatum ? for toxin production by UPLC-MS. We used new genome sequences from two strains of F. subglutinans and one strain of F. temperatum, plus genomes of other Fusarium species, to determine the presence of functional gene clusters for the synthesis of these toxins. None of the strains of either species produced fumonisins. These strains also lack FUM biosynthetic genes, but retain homologs of some genes that flank the FUM cluster in F. verticillioides. None of the F. subglutinans strains produced beauvericin, although nine of 12 F. temperatum strains did. A complete beauvericin (BEA) gene cluster was present in all three new genome sequences. BEA1 was functional in F. temperatum, but was not functional in F. subglutinans due to a large insertion and multiple mutations that resulted in premature stop codons. The pattern of mutation accumulation for BEA1 suggests that its process of inactivation is relatively recent. Thus, neither F. subglutinans nor F. temperatum can produce fumonisins and F. subglutinans cannot produce beauvericin. Variation in the ability of strains of F. temperatum to produce beauvericin requires further study and could reflect the recent shared ancestry of these two species.
  L3  - 
  JF  - Applied and Environmental Microbiology
  VL  - 
  IS  - 
  ER  -