@ARTICLE{TreeBASE2Ref17662,
author = {Elwin L. Stewart and Zhaowei Liu and Pedro W. Crous and Les J. Szabo},
title = {Phylogenetic relationships among some cercosporoid anamorphs of Mycosphaerella based on rDNA sequence analysis.},
year = {1999},
keywords = {},
doi = {10.1017/S0953756299008680},
url = {},
pmid = {},
journal = {Mycological Research},
volume = {103},
number = {11},
pages = {1491--1499},
abstract = {Partial rDNA sequences were obtained from 26 isolates representing species of Cercospora, Passalora, Paracercospora, Pseudocercospora, Ramulispora, Pseudocercosporella, and Mycocentrospora. The combined internal transcribed spacers (ITS) including the 5.8S rRNA gene and 5' end of the 25S gene (primer pairs F63/R635) on rDNA were amplified using PCR and sequenced directly. The ITS regions including the 5.8S varied in length from 502 to 595 bp. The F63/R635 region varied from 509-519 bp among isolates sequenced. Reconstructed phylogenies inferred from both regions had highly similar topologies for the taxa examined. Phylogenetic analysis of the sequences resulted in four well-supported clades corresponding to Cercospora, Paracercospora/Pseudocercospora, Passalora and Ramulispora, with bootstrap values greater than 92% for each clade. Based on the results of the analysis, a new combination for Pseudocersporella aestiva is proposed in Ramulispora, and Paracercospora is reduced to synonymy with Pseudocercospora.}
}
Citation for Study 643
Citation title:
"Phylogenetic relationships among some cercosporoid anamorphs of Mycosphaerella based on rDNA sequence analysis.".
This study was previously identified under the legacy study ID S474
(Status: Published).
Citation
Stewart E., Liu Z., Crous P.W., & Szabo L. 1999. Phylogenetic relationships among some cercosporoid anamorphs of Mycosphaerella based on rDNA sequence analysis. Mycological Research, 103(11): 1491-1499.
Authors
-
Stewart E.
-
Liu Z.
-
Crous P.W.
-
Szabo L.
Abstract
Partial rDNA sequences were obtained from 26 isolates representing species of Cercospora, Passalora, Paracercospora, Pseudocercospora, Ramulispora, Pseudocercosporella, and Mycocentrospora. The combined internal transcribed spacers (ITS) including the 5.8S rRNA gene and 5' end of the 25S gene (primer pairs F63/R635) on rDNA were amplified using PCR and sequenced directly. The ITS regions including the 5.8S varied in length from 502 to 595 bp. The F63/R635 region varied from 509-519 bp among isolates sequenced. Reconstructed phylogenies inferred from both regions had highly similar topologies for the taxa examined. Phylogenetic analysis of the sequences resulted in four well-supported clades corresponding to Cercospora, Paracercospora/Pseudocercospora, Passalora and Ramulispora, with bootstrap values greater than 92% for each clade. Based on the results of the analysis, a new combination for Pseudocersporella aestiva is proposed in Ramulispora, and Paracercospora is reduced to synonymy with Pseudocercospora.
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About this resource
- Canonical resource URI:
http://purl.org/phylo/treebase/phylows/study/TB2:S643
- Other versions:
Nexus
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- Show BibTeX reference
@ARTICLE{TreeBASE2Ref17662,
author = {Elwin L. Stewart and Zhaowei Liu and Pedro W. Crous and Les J. Szabo},
title = {Phylogenetic relationships among some cercosporoid anamorphs of Mycosphaerella based on rDNA sequence analysis.},
year = {1999},
keywords = {},
doi = {10.1017/S0953756299008680},
url = {},
pmid = {},
journal = {Mycological Research},
volume = {103},
number = {11},
pages = {1491--1499},
abstract = {Partial rDNA sequences were obtained from 26 isolates representing species of Cercospora, Passalora, Paracercospora, Pseudocercospora, Ramulispora, Pseudocercosporella, and Mycocentrospora. The combined internal transcribed spacers (ITS) including the 5.8S rRNA gene and 5' end of the 25S gene (primer pairs F63/R635) on rDNA were amplified using PCR and sequenced directly. The ITS regions including the 5.8S varied in length from 502 to 595 bp. The F63/R635 region varied from 509-519 bp among isolates sequenced. Reconstructed phylogenies inferred from both regions had highly similar topologies for the taxa examined. Phylogenetic analysis of the sequences resulted in four well-supported clades corresponding to Cercospora, Paracercospora/Pseudocercospora, Passalora and Ramulispora, with bootstrap values greater than 92% for each clade. Based on the results of the analysis, a new combination for Pseudocersporella aestiva is proposed in Ramulispora, and Paracercospora is reduced to synonymy with Pseudocercospora.}
}
- Show RIS reference
TY - JOUR
ID - 17662
AU - Stewart,Elwin L.
AU - Liu,Zhaowei
AU - Crous,Pedro W.
AU - Szabo,Les J.
T1 - Phylogenetic relationships among some cercosporoid anamorphs of Mycosphaerella based on rDNA sequence analysis.
PY - 1999
UR - http://dx.doi.org/10.1017/S0953756299008680
N2 - Partial rDNA sequences were obtained from 26 isolates representing species of Cercospora, Passalora, Paracercospora, Pseudocercospora, Ramulispora, Pseudocercosporella, and Mycocentrospora. The combined internal transcribed spacers (ITS) including the 5.8S rRNA gene and 5' end of the 25S gene (primer pairs F63/R635) on rDNA were amplified using PCR and sequenced directly. The ITS regions including the 5.8S varied in length from 502 to 595 bp. The F63/R635 region varied from 509-519 bp among isolates sequenced. Reconstructed phylogenies inferred from both regions had highly similar topologies for the taxa examined. Phylogenetic analysis of the sequences resulted in four well-supported clades corresponding to Cercospora, Paracercospora/Pseudocercospora, Passalora and Ramulispora, with bootstrap values greater than 92% for each clade. Based on the results of the analysis, a new combination for Pseudocersporella aestiva is proposed in Ramulispora, and Paracercospora is reduced to synonymy with Pseudocercospora.
L3 - 10.1017/S0953756299008680
JF - Mycological Research
VL - 103
IS - 11
SP - 1491
EP - 1499
ER -
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