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Citation for Study 650

Citation title: "The phylogenetics of mycotoxin and sclerotium production in Aspergillus flavus and Aspergillus oryzae.".

This study was previously identified under the legacy study ID S484 (Status: Published).

Citation

Geiser D., Dorner J., Horn B., & Taylor J. 2000. The phylogenetics of mycotoxin and sclerotium production in Aspergillus flavus and Aspergillus oryzae. Fungal Genetics and Biology, 31(3): 169-179.

Authors

• Geiser D.
• Dorner J.
• Horn B.
• Taylor J.

Abstract

Aspergillus flavus is a common filamentous fungus that produces aflatoxins and presents a major threat to agriculture and human health. Previous phylogenetic studies of A. flavus have shown that it consists of two subgroups, called groups I and II, and morphological studies indicated that it consists of two morphological groups based on sclerotium size, called S and L. The industrially important non-aflatoxin producing fungus A. oryzae is nested within group I. Three different gene regions, including part of a gene involved involved in aflatoxin biosynthesis (omt12), were sequenced in 33 S and L strains of A. flavus collected from various regions around the world, along with three isolates of A. oryzae, and two isolates of A. parasiticus that were used as outgroups. The production of B and G aflatoxins and cyclopiazonic acid was analyzed in the A. flavus isolates, and each isolate was identified as S or L based on sclerotium size. Phylogenetic analysis of all three genes confirmed the inference that group I and group II represent a deep divergence within A. flavus. Most group I strains produced B aflatoxins to some degree, and none produced G aflatoxins. Four out of six group II strains produced both B and G aflatoxins. All group II isolates were of the S sclerotium phenotype, whereas group I strains consisted of both S and L isolates. Based on the omt12 gene region, phylogenetic structure in sclerotium phenotype and aflatoxin production was evident within group I. Some non-aflatoxin producing isolates of group I had an omt12 allele that was identical to that found in isolates of A. oryzae.

Keywords

aflatoxin; Aspergillus section Flavi; cyclopiazonic acid; cryptic species; gene genealogies

External links

• DOI: 10.1006/fgbi.2000.1215

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S650
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref15524,
  	 author = {David M.  Geiser and Joe W.  Dorner and Bruce W.  Horn and John W.  Taylor},
  	 title = {The phylogenetics of mycotoxin and sclerotium production in Aspergillus flavus and Aspergillus oryzae.},
  	 year = {2000},
  	 keywords = {aflatoxin; Aspergillus section Flavi; cyclopiazonic acid; cryptic species; gene genealogies},
  	 doi = {10.1006/fgbi.2000.1215},
  	 url = {},
  	 pmid = {},
  	 journal = {Fungal Genetics and Biology},
  	 volume = {31},
  	 number = {3},
  	 pages = {169--179},
  	 abstract = {Aspergillus flavus is a common filamentous fungus that produces aflatoxins and presents a major threat to agriculture and human health. Previous phylogenetic studies of A. flavus have shown that it consists of two subgroups, called groups I and II, and morphological studies indicated that it consists of two morphological groups based on sclerotium size, called S and L. The industrially important non-aflatoxin producing fungus A. oryzae is nested within group I. Three different gene regions, including part of a gene involved involved in aflatoxin biosynthesis (omt12), were sequenced in 33 S and L strains of A. flavus collected from various regions around the world, along with three isolates of A. oryzae, and two isolates of A. parasiticus that were used as outgroups. The production of B and G aflatoxins and cyclopiazonic acid was analyzed in the A. flavus isolates, and each isolate was identified as S or L based on sclerotium size. Phylogenetic analysis of all three genes confirmed the inference that group I and group II represent a deep divergence within A. flavus. Most group I strains produced B aflatoxins to some degree, and none produced G aflatoxins. Four out of six group II strains produced both B and G aflatoxins. All group II isolates were of the S sclerotium phenotype, whereas group I strains consisted of both S and L isolates. Based on the omt12 gene region, phylogenetic structure in sclerotium phenotype and aflatoxin production was evident within group I. Some non-aflatoxin producing isolates of group I had an omt12 allele that was identical to that found in isolates of A. oryzae.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 15524
  AU  - Geiser,David M. 
  AU  - Dorner,Joe W. 
  AU  - Horn,Bruce W. 
  AU  - Taylor,John W. 
  T1  - The phylogenetics of mycotoxin and sclerotium production in Aspergillus flavus and Aspergillus oryzae.
  PY  - 2000
  KW  - aflatoxin; Aspergillus section Flavi; cyclopiazonic acid; cryptic species; gene genealogies
  UR  - http://dx.doi.org/10.1006/fgbi.2000.1215
  N2  - Aspergillus flavus is a common filamentous fungus that produces aflatoxins and presents a major threat to agriculture and human health. Previous phylogenetic studies of A. flavus have shown that it consists of two subgroups, called groups I and II, and morphological studies indicated that it consists of two morphological groups based on sclerotium size, called S and L. The industrially important non-aflatoxin producing fungus A. oryzae is nested within group I. Three different gene regions, including part of a gene involved involved in aflatoxin biosynthesis (omt12), were sequenced in 33 S and L strains of A. flavus collected from various regions around the world, along with three isolates of A. oryzae, and two isolates of A. parasiticus that were used as outgroups. The production of B and G aflatoxins and cyclopiazonic acid was analyzed in the A. flavus isolates, and each isolate was identified as S or L based on sclerotium size. Phylogenetic analysis of all three genes confirmed the inference that group I and group II represent a deep divergence within A. flavus. Most group I strains produced B aflatoxins to some degree, and none produced G aflatoxins. Four out of six group II strains produced both B and G aflatoxins. All group II isolates were of the S sclerotium phenotype, whereas group I strains consisted of both S and L isolates. Based on the omt12 gene region, phylogenetic structure in sclerotium phenotype and aflatoxin production was evident within group I. Some non-aflatoxin producing isolates of group I had an omt12 allele that was identical to that found in isolates of A. oryzae.
  L3  - 10.1006/fgbi.2000.1215
  JF  - Fungal Genetics and Biology
  VL  - 31
  IS  - 3
  SP  - 169
  EP  - 179
  ER  -