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Citation for Study 667

Citation title: "Reliable amplification of actin genes facilitates deep-level phylogeny.".

This study was previously identified under the legacy study ID S502 (Status: Published).

Citation

Voigt K., & Wstemeyer J. 2000. Reliable amplification of actin genes facilitates deep-level phylogeny. Microbiological Research, 155: 179-195.

Authors

• Voigt K.
• Wstemeyer J.

Abstract

The gene for actin as a highly conserved and functionally essential genetic element is developing into a major tool for phylogenetic analysis within a broad organismic range. We therefore propose a set of universally applicable primers that allow reliable amplification of actin genes. For primer construction the amino acid sequences of 57 actin genes comprising fungi, animals, plants and protists were analysed, aligned and used for the definition of six well-conserved regions which are suitable as priming sites in PCR amplification experiments. Ten primers were designed for specific in vitro amplification of actin gene fragments from a wide range of microorganisms. The corresponding gene fragments provide a strong basis to isolate nearly complete actin genes for further molecular characterization and for establishing phylogenies based on actin gene trees.

External links

• Other URL: http://www.ncbi.nlm.nih.gov/pubmed/11061186

About this resource

• Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S667
• Other versions: Nexus NeXML
• Show BibTeX reference

  		@ARTICLE{TreeBASE2Ref17998,
  	 author = {Kerstin  Voigt and J.  Wstemeyer},
  	 title = {Reliable amplification of actin genes facilitates deep-level phylogeny.},
  	 year = {2000},
  	 keywords = {},
  	 doi = {},
  	 url = {http://www.ncbi.nlm.nih.gov/pubmed/11061186},
  	 pmid = {},
  	 journal = {Microbiological Research},
  	 volume = {155},
  	 number = {},
  	 pages = {179--195},
  	 abstract = {The gene for actin as a highly conserved and functionally essential genetic element is developing into a major tool for phylogenetic analysis within a broad organismic range. We therefore propose a set of universally applicable primers that allow reliable amplification of actin genes. For primer construction the amino acid sequences of 57 actin genes comprising fungi, animals, plants and protists were analysed, aligned and used for the definition of six well-conserved regions which are suitable as priming sites in PCR amplification experiments. Ten primers were designed for specific in vitro amplification of actin gene fragments from a wide range of microorganisms. The corresponding gene fragments provide a strong basis to isolate nearly complete actin genes for further molecular characterization and for establishing phylogenies based on actin gene trees.}
  }
  		

• Show RIS reference

  		TY  - JOUR
  ID  - 17998
  AU  - Voigt,Kerstin 
  AU  - Wstemeyer,J. 
  T1  - Reliable amplification of actin genes facilitates deep-level phylogeny.
  PY  - 2000
  UR  - http://www.ncbi.nlm.nih.gov/pubmed/11061186
  N2  - The gene for actin as a highly conserved and functionally essential genetic element is developing into a major tool for phylogenetic analysis within a broad organismic range. We therefore propose a set of universally applicable primers that allow reliable amplification of actin genes. For primer construction the amino acid sequences of 57 actin genes comprising fungi, animals, plants and protists were analysed, aligned and used for the definition of six well-conserved regions which are suitable as priming sites in PCR amplification experiments. Ten primers were designed for specific in vitro amplification of actin gene fragments from a wide range of microorganisms. The corresponding gene fragments provide a strong basis to isolate nearly complete actin genes for further molecular characterization and for establishing phylogenies based on actin gene trees.
  L3  - 
  JF  - Microbiological Research
  VL  - 155
  IS  - 
  SP  - 179
  EP  - 195
  ER  -