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Citation for Study 9976

About Citation title: "Examination of molecular markers and physiological traits reveals at least two constant genotypes in the Terfezia boudieri truffle population of the Negev desert".
About This study was previously identified under the legacy study ID S2312 (Status: Published).

Citation

Ferdman Y., & Kagan-zur V. 2009. Examination of molecular markers and physiological traits reveals at least two constant genotypes in the Terfezia boudieri truffle population of the Negev desert. Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology, null.

Authors

  • Ferdman Y.
  • Kagan-zur V.

Abstract

Phylogenetic analyses have corroborated the discovery of three internal transcribed spacer (ITS) types in Terfezia boudieri isolates in the course of an earlier study in our lab and have emphasized the divergence of type 2 from types 1 and 3. The present paper describes the application of molecular and physiological tools to investigate the possible existence of cryptic species within T. boudieri. The markers used include sequences taken from the 5' end of the ribosomal large subunit gene, a chitin synthase partial sequence, β-tubulin partial sequence and amplified fragment length polymorphism (AFLP)-based markers. The markers divided the isolates into three groups, each correlated to an ITS type. Over 100 fruit bodies, among which each ITS type was represented in at least 30 specimens, were tested with each of the markers. Three of the physiological traits examined: initial growth, mycelial proliferation and mycorrhiza formation, consistently showed responses paralleling ITS type; the results presented support the division of T. boudieri into three cryptic species.

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  • Canonical resource URI: http://purl.org/phylo/treebase/phylows/study/TB2:S9976
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