@ARTICLE{TreeBASE2Ref18920,
author = {Reza Mostowfizadeh-Ghalamfarsa and Zia Banihashemi and Samad Jamali},
title = {Molecular Phylogeny of three desert truffles from Iran based on ribosomal genome},
year = {2010},
keywords = {Truffle, Ascomycota, Phylogenetics, ectomycorrhizal fungi, internal transcribed spacer of rDNA },
doi = {},
url = {http://},
pmid = {},
journal = {Rostaniha},
volume = {},
number = {},
pages = {},
abstract = {Desert truffles are hypogeous ascomycetes which can be found in semi-arid ranges of Middle East, specially Iran. So far, there are no study has been carried out on the phylogenetic relationships of these ectomycorrhizal symbionts in Iran. Therefore, samples of three desert truffle species collected from different geographical locations. Voucher specimens deposited in fungal collection of the Department of Plant Protection, Shiraz University, Shiraz, Iran. DNA was extracted from specimens using a Genomic DNA Purification kit. Regions of internal transcribed spacers 1, 2 and 5.8S genes of rDNA amplified using ITS4 and ITS1 universal primer set. Fragments of ca 600 bp recovered from PCR, purified, sequenced, edited and deposited in GenBank. The ITS rDNA sequences generated in this study compared to those of other related authentic taxa obtained from GenBank. The alignments of sequences were analysed by both distance-based and maximum likelihood methods. Among 12 specimens studied with ITS sequencing, seven isolates corresponded to Terfezia claveryi reported by other authors. Iranian T. claveryi specimens had an average identity of 99.4% (range 98.7?100%) among themselves, while all T. claveryi sequences analyzed had an average of 95.2% (range 87.2?100%). Four specimens corresponded to Trimania pinoyi, being a sister taxon of Trimania nivea, of which only one specimen could be studied. Iranian T. pinoyi specimens had an average of 99.9% identity (range 99.8-100%) among themselves, and 97.2% (range 93.1-100%) between all T. pinoyi sequences compared. All T. nivea sequences, including Iranian specimen, had an average of 96.6% identity (range 92.2-99.8%). Iranian truffles flora resembles those of Meditranian areas and South coast of Persian Gulf. Phylogenetics of other Iranian species is under investigation.}
}
Taxa for Study 10469
Citation title:
"Molecular Phylogeny of three desert truffles from Iran based on ribosomal genome".
Study name:
"Molecular Phylogeny of three desert truffles from Iran based on ribosomal genome".
This study is part of submission 10459
(Status: Published).
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