@ARTICLE{TreeBASE2Ref19091, author = {Daisuke Takuma and Shimpei Wada and Ayako Sano and Kishio Hatai}, title = {Aphanomyces sinensis sp. nov., isolated from juvenile soft-shelled turtle, Pelodiscus sinensis in Japan.}, year = {2010}, keywords = {Artificial infection; Fungal infection; Histopathology; Identification; Oomycetes}, doi = {}, url = {}, pmid = {}, journal = {Mycoscience}, volume = {48}, number = {6}, pages = {}, abstract = {A species of Aphanomyces was isolated from juvenile soft-shelled turtle, Pelodiscus sinensis cultured in Japan. Typically, an infected turtle showed small whitish maculae on the carapace. Many hyphae were observed in the epidermis. White fluffy colonies from sub-cultures of the growth were isolated on glucose-yeast (GY) agar plates. These isolates consisted of delicate, slightly wavy and moderately branched hyphae. Zoosporangia were isodiametric, namely, it was slender and same diameter when compared with the vegetative hyphae. Oogonia were abundant and approximately 18 to 25 μm in diameter with smooth surface; most were spherical or sub-spherical and only rarely pyriform. Individual oospores usually contained a single oogonium, were spherical and 15 to 22 μm in diameter. The internal structure of oospores was dominantly centric to sub-centric. Often, a clear nuclear spot was observed in the center of oospore. Antheridial branches occurred infrequently; when present the branches were predominantly diclinous. Ooginia with double or triple antheridia are predominated. The optimal growth temperature for isolates was 25?30 ?C and optimum pH was 6?9. Experimental infection tests with isolates produced small whitish macula on the carapace, and soft-shelled turtle artificially infected with the zoospores showed high mortality, especially in the high dose group. Phylogenic analysis based on the internal transcribed spacer 1 and 2 including 5.8S rRNA gene region (ITS rDNA) indicates that these isolates are as yet unidentified species of Aphanomyces. } }

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