@ARTICLE{TreeBASE2Ref19153, author = {Rodney G. Roberts and Stephen T. Reymond and Joseph Frank Bischoff}, title = {Differential gene expression in Alternaria gaisen exposed to dark and light. }, year = {2011}, keywords = {Alternaria, small-spored, aegerolysin.}, doi = {10.1007/s11557-011-0752-3}, url = {http://}, pmid = {}, journal = {Mycological Progress}, volume = {}, number = {}, pages = {}, abstract = {Character states observed during sporulation have been the basis for segregation and description of many of the small-spored species of Alternaria. Phylogenetic analyses of ITS and housekeeping genes from small-spored Alternaria spp. do not support most of the currently defined morphological species or species-groups. However, these molecular data are incongruent with a range of other species-associated physiological and pathological characters. The conidiation response of Alternaria gaisen was characterized by forward and reverse selective subtractive hybridization of cDNA produced from cultures of A. gaisen grown either in total darkness or in total darkness followed by scarification and 24h exposure to light. Differential expression of 184 transcripts was confirmed by virtual Northern analysis. Transcripts or their translation products were identified by similarity to known sequences using BLAST. Sixty-seven cloned ESTs or their hypothetically translated proteins had either no significant similarity to any GenBank accession or were similar to shotgun sequences with no known function. Multiple transcripts with similarity to ORF-1 of the AM-toxin gene were obtained from the light library. L152 is a full reading frame EST in the light library whose ORF translation has similarity to the conserved domain aegerolysin (pfam06355), a family of hemolytic proteins associated with sporulation events in bacteria and fungi. A Coca?s extract of A. gaisen mycelium was hemolytic to sheep red blood cells in an agar plate test. A set of eleven ex-type or representative isolates including A. alternata, A. gaisen, A. yaliinficiens, A. arborescens, A. tenuissima and A. brassicicola were resolved by UPGMA analysis of partial genomic sequence (415 base pairs) of L152, but were not resolved by a similar analysis of ITS sequences. Based upon these preliminary results, the sequences of putative aegerolysin homologs appear to be variable and informative, and with additional analyses of a broader and more inclusive isolate set, may have useful levels of variability for phylogenetic studies.} }

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