@ARTICLE{TreeBASE2Ref16798, author = {Henrietta Myburg and Marieka Gryzenhout and Brenda D Wingfield and Michael G. Milgroom and Shigeru Kaneko and Michael J Wingfield}, title = {DNA sequence data and morphology define Cryphonectria species in Europe, China and Japan}, year = {2004}, keywords = {}, doi = {}, url = {}, pmid = {}, journal = {Canadian Journal of Botany}, volume = {}, number = {}, pages = {}, abstract = {Cryphonectria includes important tree pathogens as well as species believed to be saprophytes. Recent phylogenetic studies have concentrated on North American and southern hemisphere Cryphonectria spp., but little is known about Asian and European taxa. In this study we identify and differentiate between the species occurring in Europe, China and Japan using morphological and phylogenetic comparisons between the Cryphonectria species. Phylogenetic comparisons were based on sequence data from the ribosomal ITS operon and two regions in the b-tubulin gene. Unknown Japanese and Chinese isolates showing different cultural features to those of C. parasitica from Japan and the USA, grouped with isolates of C. nitschkei from Quercus spp. and Rhus javanica. Isolates of C. havanensis from Q. grosseserrata, Castanopsis cuspidata, Pyrus sinensis and Eucalyptus globulus also grouped in this phylogenetic clade. We propose that Cryphonectria nitschkei and the fungus that has been referred to as C. havanensis in Japan should be treated as a single taxon. Phylogenetic and morphological data also suggest that there are two species currently representing C. radicalis in Europe. One of these species is similar to the type specimen of C. radicalis, while the other species probably is new.} }

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