@ARTICLE{TreeBASE2Ref15936, author = {Mitsuro Hyakumachi and Achmadi Priyatmojo and Mayumi Kubota and Hirokazu Fukui}, title = {New Anastomosis Groups, AG-T and AG-U, of Binucleate Rhizoctonia spp. Causing Root and Stem Rot of Cut-Flower and Miniature Roses}, year = {2005}, keywords = {}, doi = {}, url = {}, pmid = {}, journal = {Phytopathology}, volume = {95}, number = {}, pages = {}, abstract = {Root and stem rot of cut-flower roses (Rosa spp.) was observed in commercial glasshouse-grown roses in 10 prefectures of Japan from 1998 through 2001. Binucleate-like Rhizoctonia were mainly isolated from the disease plants. In total, 670 isolates were divided into two types based on cultural appearance; 168 isolates of light brown to brown type and 502 isolates of whitish type. Hyphal anastomosis reaction using representative isolates from each type revealed that light brown to brown type belonged to anastomosis group G (AG-G), while whitish type (here after called AG-CUT) failed to anastomose with tester strains of binucleate Rhizoctonia AG-A through AG-S. Neither isolates of AG-G nor AG-CUT anastomosed with tester strains of a previously reported unknown AG of binucleate Rhizoctonia collected from miniature roses (AG-MIN). In pathogenicity tests, randomly selected isolates of the 3 groups caused root and stem rot on cut-flower and miniature roses. To differentiate AG-CUT and AG-MIN from known AGs of binucleate Rhizoctonia, RFLP and sequence analyses of rDNA-ITS region were conducted. Among the 8 restriction enzymes used, HaeIII produced DNA banding patterns for AG-CUT that differed from those of tester strains and AG-MIN. Additionally, restriction profiles of AG-MIN differed from those of all tester strains. AG-G isolates from cut-flower roses had the same RFLP pattern as the tester strains of AG-G. Based on the results of hyphal anastomosis and RFLP and sequence analysis of rDNA-ITS region, we propose AG-CUT be designated AG-T and AG-MIN be designated AG-U, two new AG of binucleate Rhizoctonia. The phylogenetic tree based on the sequence data of rDNA-ITS region showed that isolates of AG-MIN were in a distinct clade from other AGs while isolates of AG-CUT were in the same clade as those of AG-A. More detailed phylogenetic analysis besides rDNA-ITS region might be necessary for AG classification of binucleate Rhizoctonia.} }

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