@ARTICLE{TreeBASE2Ref21556, author = {Wei Li and Huaigu Chen}, title = {The heterogeneity of the rDNA-ITS sequence and its phylogeny in Rhizoctonia cerealis, the cause of sharp eyespot in wheat}, year = {2013}, keywords = {anastomosis group AG-D, binucleate Rhizoctonia, evolution, haplotype}, doi = {}, url = {http://}, pmid = {}, journal = {Mycoscience}, volume = {}, number = {}, pages = {}, abstract = {Though sequence heterogeneity of the ribosomal internal transcribed spacer region (ITS) has been reported in multinucleate Rhizoctonia solani isolates, it has very rarely been reported in binucleate Rhizoctonia spp. isolates and has never been described in R. cerealis, the pathogen of wheat sharp eye-spot in China. Our goals in the present study were to investigate: (i) the presence of heterogeneity in ITS sequences in R. cerealis; (ii) the effects to the molecular identification; (iii) the phylogenetic analysis and the evolution of the ITS regions in this fungus. In this study, PCR products of the ITS region of 15 R. cerealis isolates were purified and cloned. Sequencing of 10 clones for each isolate revealed more than one different ITS sequences within one isolate, which represents the first evidence of ITS sequence heterogeneity in R. cerealis. In the phylogenetic tree based on these ITS sequences, different sequences of one isolate did not cluster in one clade, but all of the sequences of the 15 isolates were clustered in the anastomosis subgroup AG-DI, suggesting that the heterogeneity of the ITS did not affect the molecular identification of its anastomosis group. Haplotype analyses indicated that there might be three evolutionary originations of R. cerealis, and the recombination event could be the cause of different ITS sequences in one genome.} }

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