@ARTICLE{TreeBASE2Ref23303, author = {Paulo Oliveira and Ricardo Castilho and Lu?s Neves Morgado and Carlos Vila-Vi?osa and M?rio Rui Castro}, title = {Detection of an undescribed species among sporocarp collections of the edible Amanita ponderosa}, year = {2014}, keywords = {Amidella phylogeny, basidiospore, gurumelo, microscopy, molecular markers, silarca, undescribed species}, doi = {}, url = {http://}, pmid = {}, journal = {Mycologia}, volume = {}, number = {}, pages = {}, abstract = {Amanita ponderosa Mal. & Heim is a species that occurs around the Western Mediterranean, producing its edible fruitbodies between Winter and Spring. Traditionally consumed only in parts of Spain and Portugal, the gastronomic interest in this species has been spreading in recent years. As part of a wider research program to improve the scientific knowledge of this species and help in its conservation, the present study sought to uncover the intraspecific genetic diversity of the species, as represented by the nuclear ribosomal internal transcribed spacer sequences. The diversity was unusually low, but two specimens were from another species, closely related to Amanita curtipes Gilbert. Expanding the analysis to four other regions, the nuclear ribosomal large subunit sequences confirmed both results, while the remainder did not have enough resolution. The taxonomic identity of the two divergent specimens is discussed, based on basidiospore measurements, genetic distances and ecological data, and is named Amanita aff. curtipes to indicate that it is not the same species, to be described in a separate publication. Genetic markers for this undescribed species are proposed, based on the analysis of the sequence alignments for three genetic regions, to provide rapid means for its detection among Amanita ponderosa collections. The present study also discusses the phylogenetic reconstructions for Series Amidella (Gilbert) Neville & Poumarat from the point of view of identifying monophyletic clades and revising the names assigned to the sequences.} }

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