@ARTICLE{TreeBASE2Ref26072, author = {Carlos Galan Boluda and David L. Hawksworth and Pradeep Kumar Divakar and Ana Crespo and Victor J. Rico}, title = {Microchemical and molecular investigations reveal Pseudephebe species as cryptic with an environmentally modified morphology.}, year = {2016}, keywords = {Alectorioid clade, barcoding, Bryoria, cryptic species, intron, Parmeliaceae, phenotypic convergence}, doi = {}, url = {http://}, pmid = {}, journal = {The Lichenologist}, volume = {}, number = {}, pages = {}, abstract = {The results of the first molecular phylogenetic study of Pseudephebe are presented, a three-locus phylogeny. The genus is confirmed as monophyletic within the Alectorioid clade of Parmeliaceae. Two major clades were recovered, which can be assigned to the two traditional taxa, P. minuscula and P. pubescens, with modifications of the species delimitations, especially the very variable P. minuscula. These species are cryptic and cannot be confidently distinguished morphologically due to phenotypic convergence, and the use of P. pubescens aggr. is recommended for non-molecularly analyzed samples. Contrary to previous studies, specimens of both species may have indistinct pseudocyphellae and also contain lichen substances; norstictic acid was detected in c. 60 % of specimens tested. An SSU 1516 Group I intron is usually present in P. minuscula but always absent in P. pubescens. The species-level nomenclature is summarized and sequenced reference specimens (RefSpec) for both Pseudephebe species are selected. Sequences from Bryoria mariensis established that this name was a synonym of P. minuscula.} }

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