@ARTICLE{TreeBASE2Ref26351, author = {Anabela Lopes and Carla Barradas and Alan John Lander Phillips and Artur Alves}, title = {Diversity and phylogeny of Neofusicoccum species occurring on forest and urban environments in Portugal}, year = {2016}, keywords = {Botryosphaeriaceae, pathogenic, endophytic, ornamentals, host-association }, doi = {}, url = {http://}, pmid = {}, journal = {Mycosphere}, volume = {}, number = {}, pages = {}, abstract = {The genus Neofusicoccum includes species with a very wide range of geographical and host distribution, including woody plants of agricultural, forestry and economic importance. Neofusicoccum species are typically endophytes but under stress conditions cause decline and dieback symptoms. This genus comprises 27 species that are difficult to identify based on morphological features alone. In recent years much effort is being devoted to redefine species limits using multi locus sequence data, which has resulted in the identification of several cryptic species. The aim of this study was to establish phylogenetic relationships within a collection of Neofusicoccum isolates obtained from several hosts in Portugal. A total of 351 isolates was characterised by BOX-PCR fingerprinting to evaluate their overall genetic diversity. Representatives of each group identified in this analysis were selected for sequence analysis of the ribosomal internal transcribed spacer region and partial sequences of protein-coding genes, namely translation elongation factor 1-alpha and beta-tubulin. Available sequences from almost all known and well-characterized Neofusicoccum species were retrieved from GenBank and included in the phylogenetic analyses along with the sequences from the isolates under study. Maximum likelihood phylogenetic analysis of the combined three loci clearly resolved all species. Most of the isolates belong to known species, namely N. australe, N. eucalyptorum, N. luteum and N. parvum and many new host-associations were identified. } }

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