@ARTICLE{TreeBASE2Ref30516, author = {Makoto Shirakawa and Megumi Tanaka}, title = {Two new deer truffle species, Elaphomyces marmoratus and Elaphomyces fuscus spp. nov., from a secondary forest in Japan}, year = {2020}, keywords = {Hypogeous, ITS rDNA, Pinus densiflora, Quercus serrata, Sequestrate fungi}, doi = {10.1016/j.myc.2020.07.002}, url = {http://doi.org/10.1016/j.myc.2020.07.002}, pmid = {}, journal = {Mycoscience}, volume = {61}, number = {6}, pages = {315?322}, abstract = {We describe two new species of deer truffle, Elaphomyces marmoratus and E. fuscus spp. nov., collected from a secondary forest dominated by Quercus serrata, in Tokyo, Japan. Both species have morphological similarities to E. muricatus and E. granulatus; however, the former has a gleba without a purplish color and the latter has reticulated ascospores more closely resembling E. asperulus. Molecular phylogenetic analyses also support the assignment of these specimens as new species. ITS rDNA homologies with known species were low (< 91% and < 97%). In addition, phylogenetic trees using neighbor-joining and maximum likelihood methods showed that the sequences of the two new species each formed a monophyletic group within section Elaphomyces with bootstrap support of 99%. Analyses of ectomycorrhizal roots collected concurrently with the ascomata revealed that E. marmoratus is associated with at least Pinus densiflora, while E. fuscus is associated with at least Q. serrata.} }

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