@ARTICLE{TreeBASE2Ref23177,
author = {Jeffrey J Shaw and Daniel J Spakowicz and Rahul S Dalal and Jared H Davis and Nina A Lehr and Brian F Dunican and Esteban A Orellana and Alexandra Narv?ez-Trujillo and Scott A Strobel},
title = {Biosynthesis and genomic analysis of medium-chain hydrocarbon production by the endophytic fungal isolate Nigrograna mackinonnii (E5202H)},
year = {2014},
keywords = {Endophyte, natural product, Volatile Organic Compound, polyene, medium-chain hydrocarbon, biofuel, polyketide synthase},
doi = {},
url = {},
pmid = {},
journal = {Applied Microbiology and Biotechnology},
volume = {},
number = {},
pages = {},
abstract = {An endophytic fungus was isolated that produces a series of volatile natural products, including terpenes and odd chain polyenes. Phylogenetic analysis of the isolate using five loci suggests that it is closely related to Nigrograna mackinnonii CBS 674.75. The main component of the polyene series was purified and identified as (3E,5E,7E)-nona-1,3,5,7-tetraene (NTE), a novel natural product. Non-oxygenated hydrocarbons of this chain length are uncommon and desirable as gasoline-surrogate biofuels. The biosynthetic pathway for NTE production was explored using metabolic labeling and GCMS. Two-carbon incorporation 13C acetate suggests that it is derived from a polyketide synthase (PKS) followed by decarboxylation. There are several known mechanisms for such decarboxylation, though none have been discovered in fungi. Towards identifying the PKS responsible for the production of NTE, the genome of N. mackinnonii E5202H (ATCC SD-6839) was sequenced and assembled. Of the 32 PKSs present in the genome, 17 are predicted to contain sufficient domains for the production of NTE. These results exemplify the capacity of endophytic fungi to produce novel natural products that may have many uses, such as biologically derived fuels and commodity chemicals.}
}
Trees for Study 15775

Citation title:
"Biosynthesis and genomic analysis of medium-chain hydrocarbon production by the endophytic fungal isolate Nigrograna mackinonnii (E5202H)".

Study name:
"Biosynthesis and genomic analysis of medium-chain hydrocarbon production by the endophytic fungal isolate Nigrograna mackinonnii (E5202H)".

This study is part of submission 15775
(Status: Published).
Trees
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Tr120334
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Fig 12. The first of 45 equally most parsimonious trees from the Blastacervulus/Thyrinula alignment |
Tr120334 |
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Tr120338
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Fig. 61. Consensus phylogram (50 % majority rule) from the Capnodiales alignment |
Tr120338 |
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Tr120341
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Fig. 89. The single most parsimonious tree obtained from the Pseudophloeospora alignment |
Tr120341 |
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Tr120343
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Fig. 101. Distance (NJ) tree obtained from the Sonderhenia alignment |
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Tr120335
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Fig 33. Distance (NJ) tree from the Disculoides alignment |
Tr120335 |
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Tr120336
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Fig 33. The first of four equally most parsimonious trees from the Disculoides alignment |
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Tr120340
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Fig. 81. The single most parsimonious trees obtained from the Phaeothyriolum alignment |
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Tr120339
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Fig. 66. The first of seven equally most parsimonious trees obtained from the Nowamyces alignment |
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Tr120344
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Fig. 101. The first of 11 equally most parsimonious trees obtained from the Sonderhenia alignment |
Tr120344 |
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Tr120342
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Fig. 95. The first of 384 equally most parsimonious trees obtained from the Quambalaria alignment |
Tr120342 |
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Tr120337
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Fig. 40. The single most parsimonious tree obtained from the Fusculina alignment |
Tr120337 |
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