@ARTICLE{TreeBASE2Ref18673, author = {Taylor L Weiss and J S Johnston and Kazuhiro Fujisawa and Koremitsu Sumimoto and Shigeru Okada and Joe Chappell and Tim P Devarenne}, title = {Phylogenetic Placement, Genome Size, and GC-Content of the Liquid Hydrocarbon Producing Green Microalga Botryococcus Braunii var. Berkeley (Showa) (Chlorophyta)}, year = {2010}, keywords = {}, doi = {10.1111/j.1529-8817.2010.00820.x}, url = {}, pmid = {}, journal = {Journal of Phycology}, volume = {46}, number = {3}, pages = {534--540}, abstract = {We report the genome size, the GC content and perform a phylogenetic analysis on Botryococcus braunii, K?tzing, a green, colony forming, hydrocarbon-rich alga that is an attractive source for biopetroleum. While the chemistry of the hydrocarbons produced by the B race of B. braunii has been studied for many years, there is a deficiency of information concerning the molecular biology of this alga. Additionally, there has been some discrepancy as to the phylogenetic placement of the Berkeley (or Showa) strain of the B race. In order to clarify its classification, we isolated the Berkeley strain nuclear small subunit (18S) rRNA gene and beta-actin cDNA and used these sequences for phylogenetic analysis to determine that the Berkeley strain belongs to the Trebouxiophyceae class. This is in agreement with other B races of B. braunii, indicating the Berkeley strain is a true B race of B. braunii. In order to better understand molecular aspects of B. braunii, we obtained the Berkeley strain genome size as a first step in genome sequencing. Using flow cytometry, we determined the B. braunii, Berkeley genome size to be 166.2 +/- 2.2 Mb. We also estimated the GC-content of the Berkeley strain as 54.4 +/- 1.2% for expressed gene sequences.} }

• Go to search
• Citation
• Taxa
• Matrices
• Trees
• Analyses
• 

CiteULike

Delicious

Connotea