@ARTICLE{TreeBASE2Ref18682, author = {James Benjamin Beck and Michael D Windham and G. Yastkievych and Kathleen M. Pryer}, title = {A Diploids-First Approach to Species Delimitation and Interpreting Polyploid Evolution in the Fern Genus Astrolepis (Pteridaceae)}, year = {2010}, keywords = {}, doi = {10.1600/036364410791638388}, url = {}, pmid = {}, journal = {Systematic Botany}, volume = {35}, number = {2}, pages = {223--234}, abstract = {Polyploidy presents a challenge to those wishing to delimit the species within a group and reconstruct the phylogenetic relationships among these taxa. A clear understanding of the tree-like relationships among the diploid species can provide a framework upon which to reconstruct the reticulate events that gave rise to the polyploid lineages. In this study we apply this diploids-first strategy to the fern genus Astrolepis (Pteridaceae). Diploids are identified using the number of spores per sporangium and spore size. Analyses of plastid and low-copy nuclear sequence data provide well-supported estimates of phylogenetic relationships, including strong evidence for two morphologically distinctive diploid lineages not recognized in recent treatments. One of these corresponds to the type of Notholaena deltoidea, a species that has not been recognized in any modern treatment of Astrolepis. This species is resurrected here as the new combination Astrolepis deltoidea. The second novel lineage is that of a diploid initially hypothesized to exist by molecular and morphological characteristics of several established Astrolepis allopolyploids. This previously missing diploid species is described here as Astrolepis obscura.} }

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