@ARTICLE{TreeBASE2Ref26042, author = {Anabela Lopes and Alan John Lander Phillips and Artur Alves}, title = {Mating type genes as phylogenetic markers to delimit Neofusicoccum species}, year = {2016}, keywords = { Botryosphaeriaceae; Phylogeny; MAT genes; PCR-based assay; reproductive strategies;}, doi = {}, url = {http://}, pmid = {}, journal = {Fungal Biology}, volume = {}, number = {}, pages = {}, abstract = {In filamentous ascomycetes sexual reproduction is under the control of a single mating type (MAT) locus. Knowledge of MAT gene sequences has been used as a powerful tool to study evolutionary trends of mating systems, population genetics, and to establish phylogenetic relationships and species boundaries. The plant pathogenic genus Neofusicoccum includes species with a very wide geographical and host distribution, some of them with economic importance. The genus currently comprises 27 species that are difficult to identify based on morphological features alone. Thus, species differentiation is based on phylogenetic species recognition using multigene genealogies. In this study we exploited the potential of mating type genes sequences as phylogenetic markers for species delimitation in the genus Neofusicoccum. To achieve this, specific primers were designed to amplify and sequence MAT genes in several species. Phylogenetic analyses were performed on both MAT genes. For comparative purposes multigene genealogies were also done using sequences of the ribosomal internal transcribed spacer region and partial sequences of protein-coding genes, namely translation elongation factor 1-alpha and beta-tubulin, which are commonly used as phylogenetic markers in this genus. The results showed that phylogenies based on mating type genes could discriminate between the species analyzed and are in concordance with the results obtained with the more conventional multilocus phylogenetic analysis approach. Thus, MAT genes represent a powerful tool to delimit cryptic species in the genus Neofusicoccum.} }

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