@ARTICLE{TreeBASE2Ref16108, author = {K.-J. Kim and T. J. Mabry}, title = {Phylogenetic and evolutionary implications of nuclear ribosomal DNA variation in dwarf dandelions (Krigia, Lactuceae, Ateraceae).}, year = {1991}, keywords = {}, doi = {}, url = {}, pmid = {}, journal = {Plant Systematics and Evolution}, volume = {177}, number = {}, pages = {53--69}, abstract = {Restriction site and length variations of nrDNA were examined for 51 populations of seven species of Krigia. The nrDNA repeat ranged in size from 8.7 to 9.6 kilobase (kb). The transcribed region, including the two ITSs, was 5.35 kb long in all examined Krigia populations. In contrast, the size of the nontranscribed IGS varied from 3.35 to 4.25 kb. Eight different types of length-variations were identified among the 51 populations, including distinct nrDNA lengths in the tetraploid and diploid populations of both K biflora and K virginica. However, a few variations were detected among populations of the same species or within a cytotype. All populations of Krigia sect. Cymbia share a 600 bp insertion in IGS near the 18 S gene, and this feature suggests monophyly of the section. All Krigia spp. had a conjugated type of subrepeat composed of approximately 75 basepairs (bp) and 125 bp. Base modifications in the gene coding regions were highly conserved among species. Forty-five restriction sites from 15 enzymes were mapped, 24 of which were variable among populations. Only four of the variable sites occurred in the rRNA coding region while 20 variable sites were detected in the noncoding regions. Collectively, 25 enzymes generated about 66 restriction sites in each nrDNA; this amounts to about 4.3% of the nrDNA repeat. A total of 50 restriction sites was variable, 28 of which were phylogenetically informative. Phylogenetic analyses of site mutations indicated that two sections of Krigia, sect. Cymbia and sect. Krigia, are monophyletic. In addition, relationships among several species were congruent with other sources of data, such as cpDNA restriction site variation and morphology. Both length and restriction site variation supported an allopolyploid origin of the hexaploid K montana. The average sequence divergence value in Krigia nrDNA was 40 times greater than that of the chloroplast DNA. The rapid evolution of nrDNA sequences was primarily due to changes of the IGS sequences.} }

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